Candidatus Marinimicrobia bacterium MT.SAG.2

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Marinimicrobia

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8HYJ3|A0A4Y8HYJ3_9BACT 50S ribosomal protein L32 OS=Candidatus Marinimicrobia bacterium MT.SAG.2 OX=2552983 GN=rpmF PE=3 SV=1
MM1 pKa = 7.61WLFSIIDD8 pKa = 4.07LNGMAEE14 pKa = 4.39VVVTATDD21 pKa = 3.67DD22 pKa = 3.66SSASVSDD29 pKa = 3.78SFNVTILPVGDD40 pKa = 3.82DD41 pKa = 3.55PSRR44 pKa = 11.84FSLSFPLGDD53 pKa = 3.88TLNTLLPNFSWHH65 pKa = 6.82PAVDD69 pKa = 3.94PDD71 pKa = 3.61NGDD74 pKa = 3.41VIEE77 pKa = 4.05YY78 pKa = 9.81QISISTFEE86 pKa = 4.32SMEE89 pKa = 4.09SPIYY93 pKa = 10.18TEE95 pKa = 4.14SLSDD99 pKa = 3.38TFHH102 pKa = 7.29IITVPLTDD110 pKa = 3.51DD111 pKa = 3.37VKK113 pKa = 11.36YY114 pKa = 10.61YY115 pKa = 10.34WSLSANTTKK124 pKa = 10.45VNPKK128 pKa = 9.99LDD130 pKa = 3.83YY131 pKa = 9.2QTQIEE136 pKa = 4.78PIFTNNCTSRR146 pKa = 11.84GCHH149 pKa = 5.3VSGNTT154 pKa = 3.44

Molecular weight: 17.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8I6G2|A0A4Y8I6G2_9BACT NADH-quinone oxidoreductase subunit J OS=Candidatus Marinimicrobia bacterium MT.SAG.2 OX=2552983 GN=E3V36_03600 PE=3 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.02KK4 pKa = 10.25SWIAKK9 pKa = 7.53QKK11 pKa = 8.76KK12 pKa = 4.13TQKK15 pKa = 10.16FAVRR19 pKa = 11.84EE20 pKa = 3.92YY21 pKa = 11.02NRR23 pKa = 11.84CQRR26 pKa = 11.84CGRR29 pKa = 11.84ARR31 pKa = 11.84SYY33 pKa = 10.81YY34 pKa = 10.18RR35 pKa = 11.84RR36 pKa = 11.84FGLCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.87LALKK50 pKa = 10.94GEE52 pKa = 4.37IPGITKK58 pKa = 10.6ASWW61 pKa = 2.73

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1565

0

1565

497638

25

2012

318.0

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.649 ± 0.063

0.624 ± 0.02

5.804 ± 0.058

7.225 ± 0.071

4.616 ± 0.05

7.312 ± 0.057

1.683 ± 0.027

8.18 ± 0.05

6.616 ± 0.068

9.751 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.028

4.681 ± 0.047

3.672 ± 0.038

2.489 ± 0.028

4.664 ± 0.047

7.472 ± 0.047

5.168 ± 0.05

6.366 ± 0.052

1.091 ± 0.023

3.469 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski