Pelargonium zonate spot virus (isolate Tomato/Italy/1982) (PZSV) (Pelargonium zonate spot virus (isolate Tomato))

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Anulavirus; Pelargonium zonate spot virus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9DUT3|1A_PZSVT Replication protein 1a OS=Pelargonium zonate spot virus (isolate Tomato/Italy/1982) OX=650488 GN=ORF1a PE=3 SV=1
MM1 pKa = 7.82AAFTFEE7 pKa = 4.28NFLSGAYY14 pKa = 8.81TGLPIDD20 pKa = 3.93KK21 pKa = 10.11FRR23 pKa = 11.84ALGLNTEE30 pKa = 5.06DD31 pKa = 4.17YY32 pKa = 11.27DD33 pKa = 3.88EE34 pKa = 4.7QRR36 pKa = 11.84WEE38 pKa = 4.05MLVKK42 pKa = 10.6CVDD45 pKa = 3.4SGLMQFSCSRR55 pKa = 11.84DD56 pKa = 3.31EE57 pKa = 4.96ALVLLWNEE65 pKa = 3.97EE66 pKa = 4.25EE67 pKa = 4.89LPKK70 pKa = 10.92DD71 pKa = 4.08DD72 pKa = 5.47DD73 pKa = 4.96DD74 pKa = 4.04EE75 pKa = 4.76VPYY78 pKa = 10.13EE79 pKa = 4.42VPCWTPDD86 pKa = 2.94TDD88 pKa = 3.41ATVIDD93 pKa = 5.79DD94 pKa = 3.71VSEE97 pKa = 3.82WLAEE101 pKa = 4.1KK102 pKa = 10.21TSVRR106 pKa = 11.84DD107 pKa = 3.64TVVVCSDD114 pKa = 3.28YY115 pKa = 11.55DD116 pKa = 3.74AVSEE120 pKa = 4.47TPVEE124 pKa = 4.22VLSVEE129 pKa = 4.14LEE131 pKa = 3.97EE132 pKa = 6.24DD133 pKa = 3.78SEE135 pKa = 4.55EE136 pKa = 3.97DD137 pKa = 3.64TIADD141 pKa = 3.34VCLDD145 pKa = 3.28ARR147 pKa = 11.84RR148 pKa = 11.84KK149 pKa = 9.8SFRR152 pKa = 11.84DD153 pKa = 3.1YY154 pKa = 9.61FTIVEE159 pKa = 4.22EE160 pKa = 4.36EE161 pKa = 4.18FVEE164 pKa = 4.07EE165 pKa = 5.24DD166 pKa = 3.92PLISLNDD173 pKa = 3.66GNVCPVRR180 pKa = 11.84THH182 pKa = 6.17EE183 pKa = 4.33VTSLEE188 pKa = 4.33KK189 pKa = 10.34PVMLDD194 pKa = 2.65VGRR197 pKa = 11.84RR198 pKa = 11.84CDD200 pKa = 3.51RR201 pKa = 11.84VNLEE205 pKa = 4.18SLQGAINMNLPSHH218 pKa = 7.03AYY220 pKa = 10.24FDD222 pKa = 4.69DD223 pKa = 3.38TWHH226 pKa = 6.17QYY228 pKa = 8.96FVEE231 pKa = 4.81GSKK234 pKa = 11.0LDD236 pKa = 3.47VDD238 pKa = 4.86FDD240 pKa = 4.62NIRR243 pKa = 11.84LRR245 pKa = 11.84QSEE248 pKa = 4.72VFCDD252 pKa = 3.68RR253 pKa = 11.84DD254 pKa = 3.14LDD256 pKa = 4.15RR257 pKa = 11.84YY258 pKa = 8.31YY259 pKa = 10.73QPEE262 pKa = 4.46LFAGASSRR270 pKa = 11.84RR271 pKa = 11.84IGTQKK276 pKa = 10.21EE277 pKa = 3.7ALVAIRR283 pKa = 11.84KK284 pKa = 9.45RR285 pKa = 11.84NADD288 pKa = 3.54VPEE291 pKa = 4.47LADD294 pKa = 3.59SVDD297 pKa = 3.52VEE299 pKa = 4.29RR300 pKa = 11.84LSEE303 pKa = 4.23SVAKK307 pKa = 10.37KK308 pKa = 9.94FLSSYY313 pKa = 10.69VCDD316 pKa = 4.51LKK318 pKa = 11.16PVVGVMEE325 pKa = 4.54KK326 pKa = 9.75MRR328 pKa = 11.84AYY330 pKa = 8.88HH331 pKa = 6.01QKK333 pKa = 9.6WGDD336 pKa = 3.54KK337 pKa = 9.49IDD339 pKa = 3.6PMFLLKK345 pKa = 10.27EE346 pKa = 4.29HH347 pKa = 6.39NLQRR351 pKa = 11.84YY352 pKa = 5.82EE353 pKa = 4.23HH354 pKa = 6.57MIKK357 pKa = 9.59TDD359 pKa = 3.37VKK361 pKa = 8.52PTVAHH366 pKa = 5.81SMHH369 pKa = 6.59VEE371 pKa = 3.75RR372 pKa = 11.84AIPATITFHH381 pKa = 6.56GKK383 pKa = 9.69SICAGFSPSFTALFDD398 pKa = 3.86EE399 pKa = 5.18FQKK402 pKa = 11.26SLDD405 pKa = 3.69EE406 pKa = 4.08RR407 pKa = 11.84VVIPSGPISTIEE419 pKa = 3.64MDD421 pKa = 3.65FDD423 pKa = 3.66IRR425 pKa = 11.84NKK427 pKa = 10.67YY428 pKa = 8.51YY429 pKa = 11.19LEE431 pKa = 4.25VDD433 pKa = 3.8LSKK436 pKa = 10.71FDD438 pKa = 3.62KK439 pKa = 11.14SQGLLHH445 pKa = 7.4LEE447 pKa = 4.19FQRR450 pKa = 11.84KK451 pKa = 7.97ILCKK455 pKa = 9.97IGLPAHH461 pKa = 7.0LANWWCDD468 pKa = 3.57FHH470 pKa = 8.0YY471 pKa = 10.75KK472 pKa = 10.53SFISDD477 pKa = 3.2PRR479 pKa = 11.84AKK481 pKa = 10.65VSFNCSFQRR490 pKa = 11.84RR491 pKa = 11.84TGDD494 pKa = 2.73AFTFFGNTLVTMAMFSFCYY513 pKa = 9.21DD514 pKa = 2.85TRR516 pKa = 11.84QFEE519 pKa = 4.46KK520 pKa = 10.52MLFAGDD526 pKa = 3.88DD527 pKa = 3.62SLAISSSPIVGCSDD541 pKa = 3.52YY542 pKa = 10.89FVSLFNMEE550 pKa = 4.67AKK552 pKa = 10.38IMDD555 pKa = 4.49PGVPYY560 pKa = 10.25ICSKK564 pKa = 10.76FLVSDD569 pKa = 3.32EE570 pKa = 4.49LGRR573 pKa = 11.84CFSAPDD579 pKa = 4.72PIRR582 pKa = 11.84EE583 pKa = 3.99FQRR586 pKa = 11.84LGKK589 pKa = 10.39KK590 pKa = 9.87KK591 pKa = 10.21ISADD595 pKa = 3.24NDD597 pKa = 3.52DD598 pKa = 5.32ALFEE602 pKa = 4.29QYY604 pKa = 11.04VGFKK608 pKa = 10.97DD609 pKa = 4.07RR610 pKa = 11.84MSHH613 pKa = 4.76MRR615 pKa = 11.84NFSEE619 pKa = 5.65YY620 pKa = 10.26EE621 pKa = 3.76IQQLKK626 pKa = 10.47IFFNLKK632 pKa = 8.64YY633 pKa = 9.04KK634 pKa = 10.69QSGEE638 pKa = 4.18VIEE641 pKa = 5.83DD642 pKa = 3.47YY643 pKa = 10.7MGACMFYY650 pKa = 10.97SDD652 pKa = 4.0NFKK655 pKa = 10.96NFKK658 pKa = 9.39TLFTKK663 pKa = 9.93TCAPLVAALNKK674 pKa = 9.72RR675 pKa = 11.84VKK677 pKa = 10.4DD678 pKa = 3.54KK679 pKa = 10.85PFRR682 pKa = 11.84LPPSLL687 pKa = 4.07

Molecular weight: 79.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9DUT1|MVP_PZSVT Movement protein OS=Pelargonium zonate spot virus (isolate Tomato/Italy/1982) OX=650488 GN=ORF3a PE=3 SV=1
MM1 pKa = 7.5PPKK4 pKa = 10.21RR5 pKa = 11.84QNTEE9 pKa = 3.34TRR11 pKa = 11.84KK12 pKa = 9.75ARR14 pKa = 11.84QNRR17 pKa = 11.84ARR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84QQALAKK28 pKa = 9.98LARR31 pKa = 11.84EE32 pKa = 4.16FSGLSMSVEE41 pKa = 4.35RR42 pKa = 11.84SPSTSWADD50 pKa = 3.09ITEE53 pKa = 4.32SEE55 pKa = 4.55SRR57 pKa = 11.84LKK59 pKa = 10.78LIPGFTATEE68 pKa = 4.28VTFDD72 pKa = 3.61PSLTFGTHH80 pKa = 4.88TGFATAEE87 pKa = 4.02RR88 pKa = 11.84SLTVPDD94 pKa = 4.96ALLEE98 pKa = 4.41SPNLRR103 pKa = 11.84LNRR106 pKa = 11.84VAVVVLLDD114 pKa = 3.55PTVPEE119 pKa = 4.13AHH121 pKa = 7.02KK122 pKa = 9.92FWCALGDD129 pKa = 3.35RR130 pKa = 11.84WVAPSVGSFPSNAVRR145 pKa = 11.84ITGRR149 pKa = 11.84EE150 pKa = 4.01GKK152 pKa = 10.22GHH154 pKa = 6.9VIYY157 pKa = 9.91HH158 pKa = 5.32YY159 pKa = 9.94PGKK162 pKa = 8.57TVEE165 pKa = 4.57HH166 pKa = 6.32LAKK169 pKa = 10.6LRR171 pKa = 11.84VYY173 pKa = 10.77LFATDD178 pKa = 3.67YY179 pKa = 11.46AIVGNNSPVATVKK192 pKa = 10.54IFVEE196 pKa = 4.34HH197 pKa = 6.35EE198 pKa = 4.22KK199 pKa = 10.33IGQAEE204 pKa = 4.55YY205 pKa = 10.66IPLL208 pKa = 4.2

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2166

208

962

541.5

61.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.233 ± 0.569

2.493 ± 0.345

6.464 ± 1.149

6.648 ± 0.383

5.448 ± 0.696

5.91 ± 0.833

2.401 ± 0.254

4.294 ± 0.203

6.371 ± 0.377

8.68 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.43

3.555 ± 0.174

4.294 ± 0.624

2.77 ± 0.165

5.91 ± 0.372

8.495 ± 0.49

5.171 ± 0.535

7.756 ± 0.146

1.293 ± 0.1

2.678 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski