Proteome-pI 2.0 - Proteome Isoelectric Point Database

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium kluyveri

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A5N0E4|A5N0E4_CLOK5 Uncharacterized protein OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) OX=431943 GN=CKL_2578 PE=4 SV=1

MM1 pKa = 7.4LADD4 pKa = 3.4ITGDD8 pKa = 3.45GTPEE12 pKa = 4.37IISTALSGGSSGNEE26 pKa = 3.84SCDD29 pKa = 3.29VEE31 pKa = 4.65TMHH34 pKa = 6.89EE35 pKa = 4.14SSLTKK40 pKa = 10.08VDD42 pKa = 3.8NYY44 pKa = 11.51NEE46 pKa = 4.27GEE48 pKa = 4.2RR49 pKa = 11.84PGLDD53 pKa = 4.21DD54 pKa = 4.8EE55 pKa = 6.61AISQDD60 pKa = 3.21NFSFQLNGYY69 pKa = 7.41DD70 pKa = 3.66TFNMYY75 pKa = 10.62SKK77 pKa = 10.96NFDD80 pKa = 3.44KK81 pKa = 11.09SCSVDD86 pKa = 3.45LTGDD90 pKa = 3.86LSMQNAKK97 pKa = 10.47DD98 pKa = 3.12WGTEE102 pKa = 3.61VQAWMGHH109 pKa = 4.93DD110 pKa = 4.82PIYY113 pKa = 10.42TLYY116 pKa = 11.5NMDD119 pKa = 3.28NSGVYY124 pKa = 9.54GLKK127 pKa = 10.29VYY129 pKa = 10.75EE130 pKa = 4.58DD131 pKa = 3.96VSGSCHH137 pKa = 6.97ADD139 pKa = 3.04SLGG142 pKa = 3.3

Molecular weight: 15.44 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A5N4A4|A5N4A4_CLOK5 Uncharacterized protein OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) OX=431943 GN=CKL_0058 PE=4 SV=1

MM1 pKa = 7.45WMTYY5 pKa = 7.15QPKK8 pKa = 9.57KK9 pKa = 8.72KK10 pKa = 9.51QRR12 pKa = 11.84KK13 pKa = 8.04RR14 pKa = 11.84EE15 pKa = 3.69HH16 pKa = 6.25GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TLSGRR28 pKa = 11.84NVIKK32 pKa = 10.55RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.17GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.58 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3828	0	3828	1110703	30	3072	290.2	32.78

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.566 ± 0.055	1.325 ± 0.015	5.349 ± 0.034	7.005 ± 0.043	4.332 ± 0.03	6.352 ± 0.041	1.45 ± 0.017	9.988 ± 0.053	9.003 ± 0.046	8.836 ± 0.039

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.685 ± 0.016	6.219 ± 0.036	2.885 ± 0.022	2.617 ± 0.021	3.577 ± 0.03	6.629 ± 0.032	4.914 ± 0.041	6.394 ± 0.035	0.726 ± 0.015	4.146 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski