Eubacterium sp. CAG:180

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1742 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5MLY5|R5MLY5_9FIRM HPr kinase/phosphorylase OS=Eubacterium sp. CAG:180 OX=1262882 GN=hprK PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.16KK3 pKa = 9.99YY4 pKa = 10.46VCPCGYY10 pKa = 10.56VYY12 pKa = 10.77DD13 pKa = 4.46PAVGDD18 pKa = 4.12PDD20 pKa = 4.31NGVAPGTAWEE30 pKa = 4.87DD31 pKa = 3.67VPEE34 pKa = 4.42DD35 pKa = 3.74WVCPVCGLGKK45 pKa = 10.36DD46 pKa = 3.52SFTEE50 pKa = 3.76EE51 pKa = 4.22DD52 pKa = 3.19

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5MJD1|R5MJD1_9FIRM D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein OS=Eubacterium sp. CAG:180 OX=1262882 GN=BN519_01179 PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 9.98EE3 pKa = 3.77LAIKK7 pKa = 9.37MIRR10 pKa = 11.84FYY12 pKa = 10.49QRR14 pKa = 11.84KK15 pKa = 8.73ISPLFPPRR23 pKa = 11.84CRR25 pKa = 11.84YY26 pKa = 10.07YY27 pKa = 9.12PTCSQYY33 pKa = 11.64AVTAIEE39 pKa = 3.89RR40 pKa = 11.84FGIIRR45 pKa = 11.84GGLLAVWRR53 pKa = 11.84LLRR56 pKa = 11.84CNPLFPGGLDD66 pKa = 3.22PVPEE70 pKa = 4.14KK71 pKa = 10.77RR72 pKa = 11.84NKK74 pKa = 10.2KK75 pKa = 10.15KK76 pKa = 10.91GG77 pKa = 3.37

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1742

0

1742

551212

29

1757

316.4

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.813 ± 0.055

1.698 ± 0.026

6.174 ± 0.048

6.673 ± 0.055

4.326 ± 0.038

7.196 ± 0.053

1.537 ± 0.023

7.276 ± 0.046

7.693 ± 0.047

8.606 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.026

4.68 ± 0.037

3.415 ± 0.033

2.142 ± 0.028

4.061 ± 0.04

6.643 ± 0.05

5.535 ± 0.043

7.13 ± 0.044

0.727 ± 0.021

3.969 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski