Lachnotalea glycerini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnotalea

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371JI86|A0A371JI86_9FIRM Uncharacterized protein OS=Lachnotalea glycerini OX=1763509 GN=CG710_005060 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.23FKK4 pKa = 11.19NFLAVSLAFTMVAGTLSGCSSEE26 pKa = 4.4TGGEE30 pKa = 4.15VASAAVTNQGKK41 pKa = 7.35TMEE44 pKa = 4.38YY45 pKa = 9.92EE46 pKa = 4.17SKK48 pKa = 10.97LFGDD52 pKa = 5.06DD53 pKa = 4.61PITVNIIVDD62 pKa = 3.58EE63 pKa = 4.58TEE65 pKa = 3.88WQNMIDD71 pKa = 3.64NMSDD75 pKa = 3.1EE76 pKa = 4.95EE77 pKa = 4.52YY78 pKa = 10.37ISVNMEE84 pKa = 3.76VNNEE88 pKa = 4.06LFSDD92 pKa = 3.79VAIRR96 pKa = 11.84AKK98 pKa = 10.7GNSSLRR104 pKa = 11.84SVSEE108 pKa = 4.04SDD110 pKa = 2.88SDD112 pKa = 3.69RR113 pKa = 11.84YY114 pKa = 10.05SFKK117 pKa = 11.37VKK119 pKa = 10.16FDD121 pKa = 3.99KK122 pKa = 10.81YY123 pKa = 10.88QKK125 pKa = 10.45NQTCYY130 pKa = 11.05GLDD133 pKa = 3.25KK134 pKa = 11.09LVLNNVISDD143 pKa = 3.7NTYY146 pKa = 8.64MKK148 pKa = 11.2DD149 pKa = 3.26MIAFDD154 pKa = 3.41MMNYY158 pKa = 9.36IGAYY162 pKa = 9.08SSLYY166 pKa = 8.45NYY168 pKa = 10.89AVISVNGEE176 pKa = 3.56YY177 pKa = 9.6WGLYY181 pKa = 8.46IALEE185 pKa = 4.91GYY187 pKa = 10.37DD188 pKa = 4.05SSFCDD193 pKa = 3.32RR194 pKa = 11.84VFGNDD199 pKa = 4.21DD200 pKa = 3.48GNLYY204 pKa = 8.79NVKK207 pKa = 9.29MVNNGGGGPGGPDD220 pKa = 3.11QSNVDD225 pKa = 3.37GQAADD230 pKa = 3.39VSGTADD236 pKa = 3.3TQTTEE241 pKa = 4.06LTTGSDD247 pKa = 3.38TQTTDD252 pKa = 2.49SAQAQDD258 pKa = 3.7TTNEE262 pKa = 4.04NQAEE266 pKa = 4.17PPEE269 pKa = 4.18MQGGNEE275 pKa = 4.17PPSDD279 pKa = 3.84LTSALPDD286 pKa = 3.68GFDD289 pKa = 3.12IDD291 pKa = 5.73DD292 pKa = 5.24YY293 pKa = 9.6ITKK296 pKa = 10.23SAEE299 pKa = 3.89EE300 pKa = 4.4GFSLSSYY307 pKa = 10.79LEE309 pKa = 3.96EE310 pKa = 6.13LGLTEE315 pKa = 5.53ADD317 pKa = 4.27FSIPSNMDD325 pKa = 3.31LEE327 pKa = 5.78DD328 pKa = 3.77MLDD331 pKa = 3.27MVVAIKK337 pKa = 10.93NGDD340 pKa = 3.5FSDD343 pKa = 3.63EE344 pKa = 4.32SMGGGKK350 pKa = 10.38GGGGMGGSSTGGALLYY366 pKa = 10.35TDD368 pKa = 5.8DD369 pKa = 5.36EE370 pKa = 4.58ISSYY374 pKa = 11.62SSIFEE379 pKa = 3.89NTSFNHH385 pKa = 4.87TTEE388 pKa = 5.29DD389 pKa = 3.38DD390 pKa = 3.44QKK392 pKa = 11.43RR393 pKa = 11.84VIEE396 pKa = 4.79AIKK399 pKa = 10.54ALNAGEE405 pKa = 5.19NIDD408 pKa = 3.52TYY410 pKa = 11.49FNVDD414 pKa = 3.43EE415 pKa = 4.31ILRR418 pKa = 11.84YY419 pKa = 9.22LAAHH423 pKa = 5.97TVLCNFDD430 pKa = 4.3SYY432 pKa = 11.34SSSMAQNYY440 pKa = 9.04YY441 pKa = 9.99IYY443 pKa = 10.65EE444 pKa = 4.33EE445 pKa = 4.4DD446 pKa = 3.6SKK448 pKa = 11.48ISILPWDD455 pKa = 3.77YY456 pKa = 11.57NLAFGGFQSEE466 pKa = 4.73SASQCVNWAIDD477 pKa = 3.83TPVLGVDD484 pKa = 3.22MADD487 pKa = 3.51RR488 pKa = 11.84PLINQLLSNEE498 pKa = 4.49EE499 pKa = 3.99YY500 pKa = 10.3LATYY504 pKa = 10.14HH505 pKa = 7.33EE506 pKa = 4.69YY507 pKa = 10.66LQQLVDD513 pKa = 4.01GYY515 pKa = 9.14MANFSEE521 pKa = 5.04HH522 pKa = 5.87VDD524 pKa = 3.58NLVSKK529 pKa = 10.32IDD531 pKa = 4.07SYY533 pKa = 11.95VQTDD537 pKa = 3.49PTAFCTYY544 pKa = 10.31EE545 pKa = 4.01EE546 pKa = 4.5YY547 pKa = 9.31QTAVSTLKK555 pKa = 10.91SFMEE559 pKa = 4.37LRR561 pKa = 11.84MQSIQGQLDD570 pKa = 3.44GTIPSTTQGQEE581 pKa = 3.71QDD583 pKa = 3.05SSTLIDD589 pKa = 3.83ASAITLSDD597 pKa = 3.3MGSQGGGKK605 pKa = 8.04DD606 pKa = 3.35QKK608 pKa = 11.39GDD610 pKa = 3.39MPGRR614 pKa = 11.84PGQNDD619 pKa = 3.57ANTSTEE625 pKa = 4.06TNADD629 pKa = 3.33GTGDD633 pKa = 3.76TEE635 pKa = 4.57TTSDD639 pKa = 3.83TEE641 pKa = 4.52TTSDD645 pKa = 3.71TEE647 pKa = 4.55TTSDD651 pKa = 3.27TGTTSDD657 pKa = 3.67TGTTSAQQ664 pKa = 3.13

Molecular weight: 71.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255I8D4|A0A255I8D4_9FIRM Zinc metalloprotease OS=Lachnotalea glycerini OX=1763509 GN=rseP PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4293

0

4293

1421011

25

3375

331.0

37.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.425 ± 0.039

1.392 ± 0.014

5.386 ± 0.029

7.257 ± 0.046

4.272 ± 0.029

6.322 ± 0.033

1.566 ± 0.016

9.124 ± 0.045

7.675 ± 0.032

8.979 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.019

5.624 ± 0.037

2.76 ± 0.02

3.276 ± 0.019

3.369 ± 0.025

6.415 ± 0.034

5.502 ± 0.041

6.38 ± 0.026

0.86 ± 0.013

4.473 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski