Klebsiella phage SJM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lastavirus; unclassified Lastavirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0X3K6|A0A6H0X3K6_9CAUD Uncharacterized protein OS=Klebsiella phage SJM3 OX=2723759 GN=24147SJM3_00028 PE=4 SV=1
MM1 pKa = 6.89ITIPVTGYY9 pKa = 11.08AIVLACSINAGTPDD23 pKa = 5.28CIEE26 pKa = 3.97QLATGYY32 pKa = 10.03VWANAQRR39 pKa = 11.84CQEE42 pKa = 4.24EE43 pKa = 4.58LDD45 pKa = 3.59HH46 pKa = 7.01AKK48 pKa = 10.22IPGAQCEE55 pKa = 4.48SLSGITVSDD64 pKa = 4.05EE65 pKa = 4.22GTGPALARR73 pKa = 11.84MIDD76 pKa = 3.63VEE78 pKa = 4.68GMM80 pKa = 3.32

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0X3Q2|A0A6H0X3Q2_9CAUD Transcriptional regulator OS=Klebsiella phage SJM3 OX=2723759 GN=24147SJM3_00050 PE=4 SV=1
MM1 pKa = 7.83PKK3 pKa = 10.06LPSVFPIIHH12 pKa = 6.87DD13 pKa = 3.93HH14 pKa = 5.66GTDD17 pKa = 2.93AKK19 pKa = 10.75GAEE22 pKa = 4.25RR23 pKa = 11.84HH24 pKa = 6.11CLSLVKK30 pKa = 10.66GGWSAKK36 pKa = 8.95LVRR39 pKa = 11.84PAGRR43 pKa = 11.84GWRR46 pKa = 11.84VVISGYY52 pKa = 10.37RR53 pKa = 11.84GG54 pKa = 2.97

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

19345

46

4433

254.5

28.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.607 ± 0.376

0.889 ± 0.235

6.043 ± 0.188

7.092 ± 0.371

3.158 ± 0.182

7.651 ± 0.256

1.716 ± 0.163

5.025 ± 0.222

5.428 ± 0.338

7.382 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.143

4.042 ± 0.217

4.797 ± 0.166

5.056 ± 0.374

6.477 ± 0.325

5.774 ± 0.237

5.609 ± 0.194

5.857 ± 0.252

1.747 ± 0.207

2.967 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski