Rhodospira trueperi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Rhodospira

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6XJA0|A0A1G6XJA0_9PROT UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase OS=Rhodospira trueperi OX=69960 GN=SAMN05421720_101526 PE=4 SV=1
MM1 pKa = 7.34SVLAKK6 pKa = 9.84TLTRR10 pKa = 11.84GVAAGALALGLGISTAAIADD30 pKa = 3.73DD31 pKa = 4.52LEE33 pKa = 4.6PGKK36 pKa = 10.68QYY38 pKa = 11.43DD39 pKa = 3.71LGRR42 pKa = 11.84AATAEE47 pKa = 4.55EE48 pKa = 4.04IAGWDD53 pKa = 3.54IDD55 pKa = 4.09VMPDD59 pKa = 3.03GTGAPVGQGTPIEE72 pKa = 4.66GEE74 pKa = 4.31DD75 pKa = 4.33IYY77 pKa = 10.97LTQCASCHH85 pKa = 5.83GEE87 pKa = 3.82FGEE90 pKa = 5.16GMDD93 pKa = 4.5RR94 pKa = 11.84YY95 pKa = 9.36PVLFGGEE102 pKa = 4.09DD103 pKa = 3.35TLASHH108 pKa = 6.8NPVKK112 pKa = 10.22TPGSYY117 pKa = 8.65WPYY120 pKa = 10.85ASTLFDD126 pKa = 4.37YY127 pKa = 10.52IHH129 pKa = 7.09RR130 pKa = 11.84AMPFGQAQTLTNDD143 pKa = 2.94QVYY146 pKa = 10.9ALTAFLLYY154 pKa = 10.4INFVLDD160 pKa = 3.59DD161 pKa = 4.19DD162 pKa = 5.45AFVLSNEE169 pKa = 4.31NIGEE173 pKa = 3.96IEE175 pKa = 4.07MPNRR179 pKa = 11.84DD180 pKa = 3.45GFIPDD185 pKa = 4.9DD186 pKa = 4.45RR187 pKa = 11.84PDD189 pKa = 3.73AQPEE193 pKa = 4.44TVCMSDD199 pKa = 3.45CDD201 pKa = 3.66VGTEE205 pKa = 4.29VIGRR209 pKa = 11.84AAIIDD214 pKa = 3.86VTPDD218 pKa = 2.96GDD220 pKa = 4.01GQPEE224 pKa = 4.88GISLEE229 pKa = 4.15

Molecular weight: 24.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7EL61|A0A1G7EL61_9PROT Uncharacterized protein OS=Rhodospira trueperi OX=69960 GN=SAMN05421720_109118 PE=4 SV=1
MM1 pKa = 7.25SRR3 pKa = 11.84RR4 pKa = 11.84AAVYY8 pKa = 10.28LRR10 pKa = 11.84VSTTGQTTEE19 pKa = 3.79NQRR22 pKa = 11.84LEE24 pKa = 4.38LEE26 pKa = 4.34AVAARR31 pKa = 11.84CGWSIIGVYY40 pKa = 9.86EE41 pKa = 3.97DD42 pKa = 4.58AGISGAKK49 pKa = 10.13GRR51 pKa = 11.84DD52 pKa = 3.17KK53 pKa = 10.98RR54 pKa = 11.84PEE56 pKa = 3.67FDD58 pKa = 3.54RR59 pKa = 11.84LCRR62 pKa = 11.84DD63 pKa = 3.08AARR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84FDD70 pKa = 4.43IVMAWSVDD78 pKa = 3.2RR79 pKa = 11.84LGRR82 pKa = 11.84SLQDD86 pKa = 3.11LVAFLGDD93 pKa = 3.21LHH95 pKa = 8.42ASGTHH100 pKa = 6.95LYY102 pKa = 10.3LHH104 pKa = 5.79QQGIDD109 pKa = 3.13TTTPSGKK116 pKa = 10.61AMFQMLGVFAEE127 pKa = 4.92FEE129 pKa = 3.75RR130 pKa = 11.84AMIRR134 pKa = 11.84EE135 pKa = 4.1RR136 pKa = 11.84VQAGLARR143 pKa = 11.84ARR145 pKa = 11.84AQGKK149 pKa = 9.25RR150 pKa = 11.84LGRR153 pKa = 11.84PRR155 pKa = 11.84TPSTTEE161 pKa = 3.63LAIKK165 pKa = 10.4ADD167 pKa = 3.71LRR169 pKa = 11.84AGSMGIRR176 pKa = 11.84KK177 pKa = 8.8IAAKK181 pKa = 9.93HH182 pKa = 4.66GVGVSVVQRR191 pKa = 11.84LRR193 pKa = 11.84AGMDD197 pKa = 3.18SDD199 pKa = 4.47VVV201 pKa = 3.44

Molecular weight: 22.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3690

0

3690

1220506

24

3194

330.8

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.807 ± 0.064

0.877 ± 0.012

6.485 ± 0.034

5.729 ± 0.038

3.248 ± 0.027

8.841 ± 0.042

2.214 ± 0.02

4.268 ± 0.031

2.326 ± 0.031

10.406 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.018

2.176 ± 0.021

5.783 ± 0.039

2.696 ± 0.023

7.954 ± 0.046

4.771 ± 0.029

5.665 ± 0.033

7.935 ± 0.034

1.386 ± 0.017

1.931 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski