Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) (Ra/LV/RHDV/GH/1989/GE) (RHDV-FRG)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Caliciviridae; Lagovirus; Rabbit hemorrhagic disease virus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27410-2|POLG-2_RHDVF Isoform of P27410 Isoform Subgenomic capsid protein VP60 of Genome polyprotein OS=Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) OX=314536 GN=ORF1 PE=1 SV=1
MM1 pKa = 7.39EE2 pKa = 5.14GKK4 pKa = 10.19ARR6 pKa = 11.84AAPQGEE12 pKa = 4.33AAGTATTASVPGTTTDD28 pKa = 3.0GMDD31 pKa = 3.74PGVVATTSVITAEE44 pKa = 4.19NSSASIATAGIGGPPQQVDD63 pKa = 3.54QQEE66 pKa = 4.05TWRR69 pKa = 11.84TNFYY73 pKa = 10.86YY74 pKa = 10.89NDD76 pKa = 3.15VFTWSVADD84 pKa = 3.75APGSILYY91 pKa = 7.84TVQHH95 pKa = 6.35SPQNNPFTAVLSQMYY110 pKa = 9.86AGWAGGMQFRR120 pKa = 11.84FIVAGSGVFGGRR132 pKa = 11.84LVRR135 pKa = 11.84AVIPPGIEE143 pKa = 3.36IGPGLEE149 pKa = 3.93VRR151 pKa = 11.84QFPHH155 pKa = 6.28VVIDD159 pKa = 3.75ARR161 pKa = 11.84SLEE164 pKa = 4.3PVTITMPDD172 pKa = 3.5LRR174 pKa = 11.84PNMYY178 pKa = 10.15HH179 pKa = 6.3PTGDD183 pKa = 3.64PGLVPTLVLSVYY195 pKa = 10.89NNLINPFGGSTSAIQVTVEE214 pKa = 4.06TRR216 pKa = 11.84PSEE219 pKa = 4.15DD220 pKa = 3.43FEE222 pKa = 4.67FVMIRR227 pKa = 11.84APSSKK232 pKa = 9.04TVDD235 pKa = 3.84SISPAGLLTTPVLTGVGNDD254 pKa = 3.6NRR256 pKa = 11.84WNGQIVGLQPVPGGFSTCNRR276 pKa = 11.84HH277 pKa = 4.62WNLNGSTYY285 pKa = 10.49GWSSPRR291 pKa = 11.84FGDD294 pKa = 2.95IDD296 pKa = 3.54HH297 pKa = 7.02RR298 pKa = 11.84RR299 pKa = 11.84GSASYY304 pKa = 10.46SGSNATNVLQFWYY317 pKa = 10.18ANAGSAIDD325 pKa = 3.98NPISQVAPDD334 pKa = 4.89GFPDD338 pKa = 3.45MSFVPFNGPGIPAAGWVGFGAIWNSNSGAPNVTTVQAYY376 pKa = 9.18EE377 pKa = 4.06LGFATGAPGNLQPTTNTSGAQTVAKK402 pKa = 9.9SIYY405 pKa = 10.6AVVTGTAQNPAGLFVMASGIISTPNASAITYY436 pKa = 7.48TPQPDD441 pKa = 3.88RR442 pKa = 11.84IVTTPGTPAAAPVGKK457 pKa = 8.48NTPIMFASVVRR468 pKa = 11.84RR469 pKa = 11.84TGDD472 pKa = 3.2VNATAGSANGTQYY485 pKa = 10.31GTGSQPLPVTIGLSLNNYY503 pKa = 9.5SSALMPGQFFVWQLTFASGFMEE525 pKa = 4.67IGLSVDD531 pKa = 3.57GYY533 pKa = 10.65FYY535 pKa = 11.2AGTGASTTLIDD546 pKa = 3.95LTEE549 pKa = 5.34LIDD552 pKa = 3.77VRR554 pKa = 11.84PVGPRR559 pKa = 11.84PSKK562 pKa = 9.82STLVFNLGGTANGFSYY578 pKa = 11.12VV579 pKa = 3.31

Molecular weight: 60.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P27410-2|POLG-2_RHDVF Isoform of P27410 Isoform Subgenomic capsid protein VP60 of Genome polyprotein OS=Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) OX=314536 GN=ORF1 PE=1 SV=1
MM1 pKa = 7.64AFLMSEE7 pKa = 4.62FIGLGLAGAGVLSNALLRR25 pKa = 11.84RR26 pKa = 11.84QEE28 pKa = 4.08LQLQKK33 pKa = 10.83QAMEE37 pKa = 4.22NGLVLKK43 pKa = 10.49ADD45 pKa = 3.38QLGRR49 pKa = 11.84LGFNPNEE56 pKa = 4.18VKK58 pKa = 10.77NVIVGNSFSSNVRR71 pKa = 11.84LSNMHH76 pKa = 6.52NDD78 pKa = 3.1ASVVNAYY85 pKa = 9.78NVYY88 pKa = 10.11NPASNGIRR96 pKa = 11.84KK97 pKa = 9.52KK98 pKa = 10.48IKK100 pKa = 10.21SLNNSVKK107 pKa = 10.06IYY109 pKa = 8.66NTTGEE114 pKa = 4.24SSVV117 pKa = 3.3

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

3040

117

2344

1013.3

110.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.961 ± 0.662

1.842 ± 0.893

5.164 ± 1.155

3.52 ± 0.484

4.375 ± 0.222

8.75 ± 1.241

1.678 ± 0.423

5.066 ± 0.18

4.375 ± 1.331

7.763 ± 1.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.234

5.362 ± 2.041

5.822 ± 1.205

3.651 ± 0.277

4.276 ± 0.326

6.974 ± 1.106

7.895 ± 1.447

8.52 ± 0.227

1.546 ± 0.353

2.993 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski