Bacillus phage 0305phi8-36

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7KV26|A7KV26_9CAUD MazG nucleotide pyrophosphohydrolase OS=Bacillus phage 0305phi8-36 OX=458639 GN=ORF016 PE=4 SV=1
MM1 pKa = 8.28QMEE4 pKa = 4.26QYY6 pKa = 10.58IPGGDD11 pKa = 3.3STHH14 pKa = 6.82IVEE17 pKa = 5.16LTFQMGVAKK26 pKa = 10.28AKK28 pKa = 10.16VVARR32 pKa = 11.84MGGNSRR38 pKa = 11.84GGDD41 pKa = 3.12ILSCYY46 pKa = 9.54TDD48 pKa = 4.17LFSGLNVRR56 pKa = 11.84PCDD59 pKa = 3.82GEE61 pKa = 3.78NDD63 pKa = 3.74KK64 pKa = 11.64YY65 pKa = 10.71CTFEE69 pKa = 4.01EE70 pKa = 4.47EE71 pKa = 4.83EE72 pKa = 4.48YY73 pKa = 10.87EE74 pKa = 3.89QDD76 pKa = 3.09SYY78 pKa = 12.25EE79 pKa = 3.96EE80 pKa = 3.77VEE82 pKa = 4.44YY83 pKa = 10.68IRR85 pKa = 11.84FYY87 pKa = 10.16TGPYY91 pKa = 9.13YY92 pKa = 10.19EE93 pKa = 4.67EE94 pKa = 5.63HH95 pKa = 7.32DD96 pKa = 4.33LDD98 pKa = 3.78TAEE101 pKa = 5.34DD102 pKa = 4.01YY103 pKa = 11.42LIGVQIIGHH112 pKa = 5.78EE113 pKa = 4.16PEE115 pKa = 5.95DD116 pKa = 5.44DD117 pKa = 3.93DD118 pKa = 7.23DD119 pKa = 4.05EE120 pKa = 4.6

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7KV62|A7KV62_9CAUD HTH type II transcriptional regulator OS=Bacillus phage 0305phi8-36 OX=458639 GN=ORF053 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84SIGTGVVGFNPAHH16 pKa = 6.06SAAYY20 pKa = 9.95SYY22 pKa = 11.75LNIPKK27 pKa = 10.14GVVVEE32 pKa = 4.18MFGATGGRR40 pKa = 11.84GKK42 pKa = 9.39MLAVDD47 pKa = 3.96PSDD50 pKa = 3.49VHH52 pKa = 6.67LHH54 pKa = 5.62GWQEE58 pKa = 3.95AMVLAEE64 pKa = 4.05QRR66 pKa = 11.84QIDD69 pKa = 3.7AMNFKK74 pKa = 10.49NRR76 pKa = 11.84AQRR79 pKa = 11.84RR80 pKa = 11.84KK81 pKa = 8.29EE82 pKa = 3.63ARR84 pKa = 11.84EE85 pKa = 3.78NRR87 pKa = 11.84GKK89 pKa = 10.57RR90 pKa = 11.84AINHH94 pKa = 5.62RR95 pKa = 3.94

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

239

0

239

67400

39

2537

282.0

32.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.464 ± 0.143

0.688 ± 0.057

6.911 ± 0.121

7.834 ± 0.206

3.792 ± 0.08

6.493 ± 0.19

2.076 ± 0.067

6.255 ± 0.117

7.644 ± 0.149

7.398 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.197 ± 0.093

4.868 ± 0.116

3.755 ± 0.096

3.997 ± 0.113

5.016 ± 0.126

5.407 ± 0.111

6.001 ± 0.154

6.785 ± 0.161

1.15 ± 0.056

4.269 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski