Olavius algarvensis spirochete endosymbiont

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; unclassified Spirochaetales

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2385 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P5VUF2|A0A3P5VUF2_9SPIR Uncharacterized protein OS=Olavius algarvensis spirochete endosymbiont OX=260710 GN=S1OALGB6SA_435 PE=4 SV=1
NN1 pKa = 6.77YY2 pKa = 10.6SVIEE6 pKa = 3.95ALNWYY11 pKa = 10.02AYY13 pKa = 10.36VSNNPVRR20 pKa = 11.84YY21 pKa = 9.5VDD23 pKa = 3.43PTGMFNTSEE32 pKa = 4.08IDD34 pKa = 3.04EE35 pKa = 5.49DD36 pKa = 4.35YY37 pKa = 10.73PKK39 pKa = 10.75SAEE42 pKa = 4.47FINNLQPSSDD52 pKa = 4.06DD53 pKa = 3.17YY54 pKa = 12.0DD55 pKa = 3.94AFEE58 pKa = 5.17EE59 pKa = 4.27YY60 pKa = 10.85GQASKK65 pKa = 11.52NDD67 pKa = 3.03VDD69 pKa = 4.27RR70 pKa = 11.84AFTEE74 pKa = 4.5GNPPNVIAEE83 pKa = 4.31SLGGDD88 pKa = 3.13NGAFQPDD95 pKa = 4.28SNSEE99 pKa = 4.04DD100 pKa = 3.28IIINKK105 pKa = 9.55DD106 pKa = 3.22LLEE109 pKa = 4.27AFEE112 pKa = 5.63DD113 pKa = 4.76GVSGSDD119 pKa = 3.79LLLEE123 pKa = 4.19STIKK127 pKa = 10.82HH128 pKa = 5.03EE129 pKa = 4.27TTHH132 pKa = 6.68YY133 pKa = 10.89FDD135 pKa = 5.3DD136 pKa = 4.66QDD138 pKa = 5.02GEE140 pKa = 4.5DD141 pKa = 3.85YY142 pKa = 10.9PGEE145 pKa = 4.05EE146 pKa = 3.94GQAFEE151 pKa = 4.12EE152 pKa = 4.22QVYY155 pKa = 10.76GEE157 pKa = 5.47DD158 pKa = 4.64IDD160 pKa = 4.14TLEE163 pKa = 3.9QAEE166 pKa = 4.7KK167 pKa = 10.15YY168 pKa = 10.5LEE170 pKa = 4.35RR171 pKa = 11.84EE172 pKa = 4.08

Molecular weight: 19.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P5VHT0|A0A3P5VHT0_9SPIR Pyrophosphate-dependent fructose 6-phosphate-1-kinase (Fragment) OS=Olavius algarvensis spirochete endosymbiont OX=260710 GN=S1OALGB6SA_1032 PE=3 SV=1
MM1 pKa = 7.42NLFQSTHH8 pKa = 5.08PRR10 pKa = 11.84GVRR13 pKa = 11.84RR14 pKa = 11.84SSGGSIGPLLWFQSTHH30 pKa = 5.32PRR32 pKa = 11.84GVRR35 pKa = 11.84QQRR38 pKa = 11.84NGLKK42 pKa = 10.03INLAGVSIHH51 pKa = 6.59APARR55 pKa = 11.84GATAWPAGQKK65 pKa = 9.62LASPEE70 pKa = 4.05FQSTHH75 pKa = 5.02PRR77 pKa = 11.84GVRR80 pKa = 11.84RR81 pKa = 11.84ARR83 pKa = 11.84PVAGG87 pKa = 3.63

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2385

0

2385

644439

20

1962

270.2

30.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.78 ± 0.053

0.898 ± 0.017

5.412 ± 0.038

6.919 ± 0.057

4.297 ± 0.036

7.469 ± 0.05

1.795 ± 0.023

7.35 ± 0.048

5.363 ± 0.047

10.198 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.023

4.209 ± 0.03

4.021 ± 0.031

2.79 ± 0.027

6.298 ± 0.04

7.197 ± 0.044

4.864 ± 0.033

6.435 ± 0.044

1.27 ± 0.023

3.07 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski