Kosmotoga sp.

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Kosmotogales; Kosmotogaceae; Kosmotoga; unclassified Kosmotoga

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D0TAZ2|A0A5D0TAZ2_9BACT B12-binding domain-containing radical SAM protein OS=Kosmotoga sp. OX=1955248 GN=FXF54_00930 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.35KK3 pKa = 10.46LLILSTIIVAVMALISCTATMNILPKK29 pKa = 10.5SILIDD34 pKa = 4.04DD35 pKa = 3.9SHH37 pKa = 7.71NNQFGYY43 pKa = 10.55VDD45 pKa = 3.89EE46 pKa = 5.28NFFATMINTLEE57 pKa = 4.27GEE59 pKa = 4.29NYY61 pKa = 9.93QVDD64 pKa = 4.21FTSDD68 pKa = 3.02VGFSPDD74 pKa = 3.35QYY76 pKa = 11.3DD77 pKa = 3.38ILIIPTPVSLYY88 pKa = 11.02SNTEE92 pKa = 3.46INQVKK97 pKa = 10.62SFVQNGNKK105 pKa = 9.77IIVLGEE111 pKa = 3.82FYY113 pKa = 10.66SYY115 pKa = 11.51YY116 pKa = 10.96DD117 pKa = 3.57NSEE120 pKa = 3.94LNYY123 pKa = 10.22ILNQVGADD131 pKa = 3.49MQFDD135 pKa = 3.57NSLITDD141 pKa = 3.89SVNNYY146 pKa = 9.91DD147 pKa = 6.13NNDD150 pKa = 3.17EE151 pKa = 4.1WPEE154 pKa = 3.64ISLFTPHH161 pKa = 7.14PTTDD165 pKa = 3.31SLNSIVLFAASSLNVFGDD183 pKa = 4.15AIKK186 pKa = 10.32IAEE189 pKa = 4.22SSSINLVEE197 pKa = 4.48TFDD200 pKa = 3.94SSTFNEE206 pKa = 4.41SGLKK210 pKa = 9.97AGLMEE215 pKa = 4.35NQDD218 pKa = 3.78YY219 pKa = 11.15DD220 pKa = 4.05SQTTAYY226 pKa = 10.13VSVAAVDD233 pKa = 3.95KK234 pKa = 10.78IGNGKK239 pKa = 8.83VVAVTDD245 pKa = 3.73VNLFSDD251 pKa = 4.4DD252 pKa = 3.41VDD254 pKa = 4.66DD255 pKa = 5.95FFPSLDD261 pKa = 3.47SMLNVADD268 pKa = 4.62NKK270 pKa = 11.03DD271 pKa = 3.53FLINIVNWW279 pKa = 3.5

Molecular weight: 30.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D0SFV1|A0A5D0SFV1_9BACT 30S ribosomal protein S18 OS=Kosmotoga sp. OX=1955248 GN=rpsR PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.18QPSRR9 pKa = 11.84SKK11 pKa = 10.76RR12 pKa = 11.84KK13 pKa = 8.13RR14 pKa = 11.84THH16 pKa = 6.16GFLARR21 pKa = 11.84KK22 pKa = 6.9KK23 pKa = 7.78TKK25 pKa = 9.6AGKK28 pKa = 10.12RR29 pKa = 11.84ILANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 10.0KK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84IAAA44 pKa = 3.93

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2971

0

2971

932970

13

3461

314.0

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.535 ± 0.04

0.792 ± 0.016

5.443 ± 0.038

7.796 ± 0.049

5.097 ± 0.04

6.471 ± 0.033

1.57 ± 0.017

8.699 ± 0.034

8.177 ± 0.064

9.521 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.02

5.358 ± 0.033

3.521 ± 0.026

2.227 ± 0.018

4.103 ± 0.032

6.525 ± 0.036

5.087 ± 0.038

6.566 ± 0.031

1.021 ± 0.018

4.059 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski