Shewanella sp. P1-14-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; unclassified Shewanella

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q0ITN9|A0A0Q0ITN9_9GAMM Peptidyl-prolyl cis-trans isomerase OS=Shewanella sp. P1-14-1 OX=1723761 GN=fkpA_1 PE=3 SV=1
MM1 pKa = 7.27KK2 pKa = 10.25RR3 pKa = 11.84IYY5 pKa = 10.9SLALCFSLIGLTACSDD21 pKa = 3.99DD22 pKa = 5.39DD23 pKa = 5.89DD24 pKa = 4.37DD25 pKa = 5.42TITAADD31 pKa = 3.65SEE33 pKa = 5.06APVVTYY39 pKa = 11.24ADD41 pKa = 2.94IRR43 pKa = 11.84VIHH46 pKa = 6.79AGSDD50 pKa = 3.13APMVNVTADD59 pKa = 3.4GATLLSDD66 pKa = 3.22VDD68 pKa = 3.97YY69 pKa = 11.69AMSSGLLEE77 pKa = 4.33VATNTYY83 pKa = 10.39AIAVDD88 pKa = 3.55AQLADD93 pKa = 3.67GTTLTVLEE101 pKa = 4.86AEE103 pKa = 4.51LAVAEE108 pKa = 4.28DD109 pKa = 3.69MEE111 pKa = 4.62YY112 pKa = 9.77TAVALGKK119 pKa = 10.73VSDD122 pKa = 3.87EE123 pKa = 3.99TLMLKK128 pKa = 10.61LIANEE133 pKa = 4.15EE134 pKa = 4.09SDD136 pKa = 3.24ITADD140 pKa = 3.42NARR143 pKa = 11.84VQVLHH148 pKa = 6.2ATPSVGLVDD157 pKa = 4.16IYY159 pKa = 10.48VTEE162 pKa = 4.17PMADD166 pKa = 3.04ITMMDD171 pKa = 3.56PTLSANYY178 pKa = 8.89MDD180 pKa = 5.67NSDD183 pKa = 3.72QLEE186 pKa = 4.58VPTSDD191 pKa = 3.29YY192 pKa = 10.83QIRR195 pKa = 11.84ITPAGDD201 pKa = 3.02KK202 pKa = 10.38TVVFDD207 pKa = 4.2SGTVNLAAGMDD218 pKa = 3.95YY219 pKa = 10.56FISAIPNEE227 pKa = 4.19WSGDD231 pKa = 3.78SPVALLVALPEE242 pKa = 4.39GQVLLNDD249 pKa = 3.85VNSGSDD255 pKa = 2.97IRR257 pKa = 11.84VVHH260 pKa = 6.57AVADD264 pKa = 4.18APAVDD269 pKa = 3.83VFLDD273 pKa = 4.37GSTTPAIDD281 pKa = 3.27MLSFGNFAGYY291 pKa = 10.96VNIPEE296 pKa = 4.37GMHH299 pKa = 5.52TVTVAADD306 pKa = 3.46ADD308 pKa = 3.84NSVEE312 pKa = 4.29VIKK315 pKa = 10.93DD316 pKa = 3.35AAVDD320 pKa = 3.9LMQGASYY327 pKa = 11.02SVLAVGSLADD337 pKa = 3.92NDD339 pKa = 3.36ISPWAFAEE347 pKa = 4.08HH348 pKa = 5.85TRR350 pKa = 11.84RR351 pKa = 11.84IATEE355 pKa = 3.21ARR357 pKa = 11.84LNVIHH362 pKa = 7.15ASYY365 pKa = 10.87SAGNVDD371 pKa = 4.67VYY373 pKa = 9.95LTPTADD379 pKa = 4.32ISDD382 pKa = 4.72ADD384 pKa = 3.9PALTDD389 pKa = 3.6VPFKK393 pKa = 10.67AASGSLSVAPGDD405 pKa = 3.69YY406 pKa = 9.28TVSVTVTGTKK416 pKa = 9.01TVAIGPLAVSLAGNGLYY433 pKa = 10.42SVAAVDD439 pKa = 4.54AEE441 pKa = 4.38NDD443 pKa = 3.16TSMFSVILMDD453 pKa = 4.87DD454 pKa = 4.49FVAEE458 pKa = 4.21EE459 pKa = 3.94

Molecular weight: 47.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q0G5K1|A0A0Q0G5K1_9GAMM Uroporphyrinogen III methylase OS=Shewanella sp. P1-14-1 OX=1723761 GN=cysG_2 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.3VIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.35GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4159

0

4159

1398972

29

4187

336.4

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.732 ± 0.038

1.006 ± 0.015

5.77 ± 0.041

5.975 ± 0.037

4.157 ± 0.023

6.652 ± 0.042

2.225 ± 0.02

6.58 ± 0.029

5.206 ± 0.031

10.116 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.021

4.645 ± 0.032

3.801 ± 0.022

4.876 ± 0.036

3.999 ± 0.032

6.902 ± 0.035

5.604 ± 0.033

6.828 ± 0.03

1.198 ± 0.016

3.102 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski