Citrus tristeza virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q66239|Q66239_9CLOS 65-kDa protein OS=Citrus tristeza virus OX=12162 PE=3 SV=1
EEE2 pKa = 4.64PPLLTRR8 pKa = 11.84VTYYY12 pKa = 8.25NRR14 pKa = 11.84LAFGVVRR21 pKa = 11.84SQAIPPRR28 pKa = 11.84KKK30 pKa = 9.64SLQEEE35 pKa = 3.78LLSYYY40 pKa = 10.31EE41 pKa = 3.78RR42 pKa = 11.84NYYY45 pKa = 10.99FIKKK49 pKa = 9.63EEE51 pKa = 3.96FVGPSEEE58 pKa = 4.47GRR60 pKa = 11.84AMAAAVIEEE69 pKa = 4.57CFKKK73 pKa = 9.52EEE75 pKa = 3.66EE76 pKa = 4.01AKKK79 pKa = 9.57RR80 pKa = 11.84CDDD83 pKa = 3.35ISLTEEE89 pKa = 3.86NILKKK94 pKa = 9.53LDDD97 pKa = 3.18KK98 pKa = 10.2TPCQIKKK105 pKa = 10.09VHHH108 pKa = 6.66EEE110 pKa = 4.0KKK112 pKa = 10.82PFSVEEE118 pKa = 3.92EE119 pKa = 3.95ISNFKKK125 pKa = 11.12MVKKK129 pKa = 9.87DDD131 pKa = 3.9KKK133 pKa = 11.12KKK135 pKa = 10.73DDD137 pKa = 3.89DD138 pKa = 4.36SLSKKK143 pKa = 10.9HH144 pKa = 5.88AAQNIMFHHH153 pKa = 6.55KK154 pKa = 10.42KK155 pKa = 9.79INAIFSPCFDDD166 pKa = 3.38EE167 pKa = 5.07KKK169 pKa = 11.24RR170 pKa = 11.84VLSSLNDDD178 pKa = 3.4IVFFTEEE185 pKa = 3.75TNAGLAEEE193 pKa = 4.85IRR195 pKa = 11.84RR196 pKa = 11.84IIGDDD201 pKa = 3.93DD202 pKa = 3.51DD203 pKa = 4.03LFVGEEE209 pKa = 4.09DDD211 pKa = 4.37SKKK214 pKa = 10.7DDD216 pKa = 3.28KK217 pKa = 11.26QDDD220 pKa = 3.57FIKKK224 pKa = 10.06EE225 pKa = 4.2YY226 pKa = 10.86EE227 pKa = 3.91TLYYY231 pKa = 10.94EEE233 pKa = 5.02GFDDD237 pKa = 4.74EEE239 pKa = 5.82LDDD242 pKa = 3.32WMEEE246 pKa = 3.84EEE248 pKa = 4.0YY249 pKa = 10.18ARR251 pKa = 11.84ATTLDDD257 pKa = 3.59QLSFSVDDD265 pKa = 3.07QRR267 pKa = 11.84RR268 pKa = 11.84SGGSNTWIGNSLVTLGILSLYYY290 pKa = 10.24YY291 pKa = 10.4DD292 pKa = 3.44SKKK295 pKa = 11.09DDD297 pKa = 4.45LLVSGDDD304 pKa = 3.82DD305 pKa = 3.53LIYYY309 pKa = 10.53SEEE312 pKa = 4.14KK313 pKa = 9.81SNFSSEEE320 pKa = 3.85CLEEE324 pKa = 4.16GFEEE328 pKa = 4.44KKK330 pKa = 10.18MSPSVPYYY338 pKa = 9.38CSKKK342 pKa = 10.45VVQTGNKKK350 pKa = 7.69CFVPDDD356 pKa = 4.2YYY358 pKa = 11.44KK359 pKa = 11.07LVKKK363 pKa = 10.68GAPQNKKK370 pKa = 7.99TDDD373 pKa = 3.46EEE375 pKa = 4.41FEEE378 pKa = 5.94FTSFKKK384 pKa = 11.42DD385 pKa = 3.33TQDDD389 pKa = 3.45GDDD392 pKa = 3.61VVLEEE397 pKa = 4.06KK398 pKa = 11.11KKK400 pKa = 11.18LVEEE404 pKa = 4.6KKK406 pKa = 10.59YY407 pKa = 10.86FASGTTMPALCAIHHH422 pKa = 6.44VRR424 pKa = 11.84SNFLSFEEE432 pKa = 3.97LFPFIRR438 pKa = 11.84GWYYY442 pKa = 9.18VDDD445 pKa = 3.87LKKK448 pKa = 10.74RR449 pKa = 11.84QLRR452 pKa = 11.84KKK454 pKa = 8.6TNLICEEE461 pKa = 3.93VVYYY465 pKa = 9.94DD466 pKa = 3.34RR467 pKa = 11.84VSYYY471 pKa = 10.3SYYY474 pKa = 10.65DDD476 pKa = 3.66PFTKKK481 pKa = 10.16DDD483 pKa = 3.8NDDD486 pKa = 4.29DD487 pKa = 3.97VDDD490 pKa = 3.88DD491 pKa = 5.13GQAGEEE497 pKa = 4.31ATG

Molecular weight: 56.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q89624|Q89624_9CLOS 6-kDa protein OS=Citrus tristeza virus OX=12162 GN=p6 PE=4 SV=1
MM1 pKa = 7.96DD2 pKa = 4.83CVIQGFLTFLVGIAVFCAFAGLIIIVITIYY32 pKa = 10.51RR33 pKa = 11.84CTIKK37 pKa = 10.21PVRR40 pKa = 11.84SASPYY45 pKa = 7.69GTHH48 pKa = 6.02ATVV51 pKa = 3.06

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6023

51

3124

547.5

61.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.093 ± 0.313

2.324 ± 0.192

5.96 ± 0.305

4.964 ± 0.283

5.396 ± 0.399

5.678 ± 0.257

1.992 ± 0.247

4.383 ± 0.449

4.914 ± 0.343

9.796 ± 0.547

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.37

3.852 ± 0.401

3.852 ± 0.216

1.76 ± 0.165

7.388 ± 0.852

10.327 ± 0.627

5.429 ± 0.475

9.514 ± 0.538

0.664 ± 0.112

3.752 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski