Leucobacter sp. OH2974_COT-288

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1VPY5|A0A3P1VPY5_9MICO DUF3710 domain-containing protein OS=Leucobacter sp. OH2974_COT-288 OX=2491050 GN=EII31_01655 PE=4 SV=1
MM1 pKa = 7.14ATDD4 pKa = 3.43YY5 pKa = 10.26DD6 pKa = 3.9APRR9 pKa = 11.84KK10 pKa = 9.66SVEE13 pKa = 4.4DD14 pKa = 3.42EE15 pKa = 4.3AEE17 pKa = 4.24SIEE20 pKa = 3.98ALKK23 pKa = 11.02DD24 pKa = 3.37RR25 pKa = 11.84GPEE28 pKa = 3.74SGAAQMDD35 pKa = 4.06AEE37 pKa = 4.78DD38 pKa = 4.25ADD40 pKa = 3.98NPGFEE45 pKa = 4.77LAGADD50 pKa = 3.83LSNVEE55 pKa = 4.39LDD57 pKa = 3.93VVVLPQQEE65 pKa = 4.9DD66 pKa = 4.09EE67 pKa = 4.68FTCTNCFLVRR77 pKa = 11.84HH78 pKa = 6.22HH79 pKa = 6.36SQYY82 pKa = 11.49DD83 pKa = 3.41HH84 pKa = 7.07EE85 pKa = 6.94GDD87 pKa = 3.95LGPVCAEE94 pKa = 4.17CALL97 pKa = 3.96

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1VPQ8|A0A3P1VPQ8_9MICO Glycosyltransferase OS=Leucobacter sp. OH2974_COT-288 OX=2491050 GN=EII31_01560 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.68GRR40 pKa = 11.84SKK42 pKa = 10.58ISAA45 pKa = 3.55

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1426

0

1426

481477

32

2155

337.6

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.443 ± 0.094

0.664 ± 0.016

4.822 ± 0.045

5.997 ± 0.064

3.374 ± 0.045

7.843 ± 0.055

2.028 ± 0.028

4.864 ± 0.042

3.474 ± 0.05

10.577 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.832 ± 0.025

2.865 ± 0.038

4.738 ± 0.04

4.453 ± 0.063

5.654 ± 0.065

5.314 ± 0.038

6.354 ± 0.055

8.227 ± 0.058

1.22 ± 0.024

2.26 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski