Lates calcarifer birnavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Blosnavirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8PKK9|A0A4P8PKK9_9VIRU Structural polyprotein OS=Lates calcarifer birnavirus OX=2583366 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 4.76MTTKK6 pKa = 9.68STVVPYY12 pKa = 10.54LKK14 pKa = 10.46SLLMPDD20 pKa = 4.22TGPASIPDD28 pKa = 3.97DD29 pKa = 4.27NIEE32 pKa = 3.83RR33 pKa = 11.84HH34 pKa = 5.44TIKK37 pKa = 10.64SEE39 pKa = 3.53PTTYY43 pKa = 11.15NLTVGEE49 pKa = 4.47SGSGIIVLYY58 pKa = 9.76PNSPNSPLGAHH69 pKa = 6.33YY70 pKa = 10.55KK71 pKa = 9.79RR72 pKa = 11.84DD73 pKa = 3.51KK74 pKa = 11.16NNPSKK79 pKa = 11.14LIFDD83 pKa = 4.34RR84 pKa = 11.84AITTSQDD91 pKa = 2.72LKK93 pKa = 10.41KK94 pKa = 10.47AYY96 pKa = 10.52NFGRR100 pKa = 11.84LVSRR104 pKa = 11.84VLSAKK109 pKa = 10.49SSTLPSGVYY118 pKa = 10.09SLNGTLSAVTYY129 pKa = 9.99IGSLSEE135 pKa = 5.66IGDD138 pKa = 3.83LDD140 pKa = 3.96YY141 pKa = 11.9NKK143 pKa = 10.17ILSTTANHH151 pKa = 6.34NDD153 pKa = 3.07KK154 pKa = 10.94VGNVLARR161 pKa = 11.84EE162 pKa = 4.39GVTVLALPTGFDD174 pKa = 3.56LQFTRR179 pKa = 11.84MGDD182 pKa = 3.57DD183 pKa = 3.6SPSSEE188 pKa = 4.04GAATVDD194 pKa = 3.91PSSQPRR200 pKa = 11.84VYY202 pKa = 10.41SAQAEE207 pKa = 4.36TSQTITTGTSKK218 pKa = 11.09NLIAHH223 pKa = 6.55NLDD226 pKa = 4.42AITPVTVQTHH236 pKa = 5.08VSLTTTSPTSVEE248 pKa = 3.53ISLIGLDD255 pKa = 3.41EE256 pKa = 4.83SKK258 pKa = 11.08VVTHH262 pKa = 6.18NAIISGTTGTLTQDD276 pKa = 2.81VTAIFTQADD285 pKa = 3.27IKK287 pKa = 10.96QPIVAMTVKK296 pKa = 9.05VTAAADD302 pKa = 3.16ITAAQIQTRR311 pKa = 11.84TTVHH315 pKa = 6.36GGDD318 pKa = 3.47APGVLRR324 pKa = 11.84PVTIVAYY331 pKa = 7.37EE332 pKa = 3.98TLAEE336 pKa = 3.99NSILTLAGVSNYY348 pKa = 9.39EE349 pKa = 4.47LIPNPEE355 pKa = 3.79LSKK358 pKa = 11.29NIVATYY364 pKa = 10.52GKK366 pKa = 10.5LNPQEE371 pKa = 3.9MLYY374 pKa = 9.81TKK376 pKa = 10.49VVLSHH381 pKa = 7.03RR382 pKa = 11.84DD383 pKa = 3.5EE384 pKa = 5.57IGLKK388 pKa = 9.78SVWATDD394 pKa = 3.24QYY396 pKa = 11.8KK397 pKa = 10.83DD398 pKa = 3.8FRR400 pKa = 11.84AYY402 pKa = 9.6FAEE405 pKa = 4.18VADD408 pKa = 3.77ISKK411 pKa = 9.81PLQIAGAFGWGDD423 pKa = 3.1VLGFLRR429 pKa = 11.84RR430 pKa = 11.84WVFPGINAVLPVAAPLTNFIGEE452 pKa = 5.09KK453 pKa = 9.55IQQAYY458 pKa = 9.04PEE460 pKa = 4.62AASGHH465 pKa = 6.03PRR467 pKa = 11.84AASGRR472 pKa = 11.84PRR474 pKa = 11.84AAGTFRR480 pKa = 11.84PFRR483 pKa = 11.84PMACDD488 pKa = 3.34EE489 pKa = 4.28VDD491 pKa = 3.44HH492 pKa = 6.69KK493 pKa = 11.22FNSPALVLPGDD504 pKa = 3.76WSFLTPEE511 pKa = 4.46GTSKK515 pKa = 10.71EE516 pKa = 4.02VLPNGTLVVGSTGEE530 pKa = 3.77EE531 pKa = 4.11GAIKK535 pKa = 9.26ITDD538 pKa = 3.39SVTIVSSGAWLRR550 pKa = 11.84KK551 pKa = 9.35LLPCLNPTPLASDD564 pKa = 3.95RR565 pKa = 11.84PYY567 pKa = 10.49IPPHH571 pKa = 4.64MRR573 pKa = 11.84GRR575 pKa = 11.84PEE577 pKa = 4.56SPTCTGAPTKK587 pKa = 9.76FTTHH591 pKa = 6.74RR592 pKa = 11.84GALFPTVAFGEE603 pKa = 4.81GTSVAQTYY611 pKa = 9.47VALPGDD617 pKa = 4.46YY618 pKa = 10.01RR619 pKa = 11.84HH620 pKa = 6.75LMAPNLRR627 pKa = 11.84NEE629 pKa = 4.18WAPTQDD635 pKa = 2.95GFKK638 pKa = 10.38VYY640 pKa = 10.55GMTHH644 pKa = 5.49HH645 pKa = 7.49TIHH648 pKa = 7.71DD649 pKa = 3.64NGKK652 pKa = 9.23PSRR655 pKa = 11.84KK656 pKa = 9.78LNIQVATGGNITILPVDD673 pKa = 3.62KK674 pKa = 11.09VQWDD678 pKa = 3.73KK679 pKa = 11.65EE680 pKa = 4.4DD681 pKa = 4.18PGLATVTTSWNGFPAVVGQSGSLALALASNLDD713 pKa = 3.92YY714 pKa = 10.82FPQAVFTGCLNNGQVQPVAFGCMKK738 pKa = 10.54AQAAHH743 pKa = 6.83ALGLKK748 pKa = 10.27LCGFTTGRR756 pKa = 11.84DD757 pKa = 3.3EE758 pKa = 6.86DD759 pKa = 3.85IPLYY763 pKa = 9.57TAEE766 pKa = 4.73EE767 pKa = 4.25ALQQASTYY775 pKa = 9.89RR776 pKa = 11.84AEE778 pKa = 4.49LPYY781 pKa = 9.76STRR784 pKa = 11.84DD785 pKa = 3.09SLYY788 pKa = 10.95NGFFYY793 pKa = 10.9CMAADD798 pKa = 3.82TSLDD802 pKa = 3.73EE803 pKa = 4.55EE804 pKa = 4.69LDD806 pKa = 4.52EE807 pKa = 4.3IFKK810 pKa = 10.09WADD813 pKa = 3.26EE814 pKa = 4.14NLGSSEE820 pKa = 4.23EE821 pKa = 4.09QVDD824 pKa = 4.57TYY826 pKa = 11.99ASFPKK831 pKa = 10.57SKK833 pKa = 10.91DD834 pKa = 3.13VTTTDD839 pKa = 3.15ATDD842 pKa = 3.08QALALMEE849 pKa = 4.35EE850 pKa = 4.56AAEE853 pKa = 4.75SDD855 pKa = 3.58PQLAKK860 pKa = 10.63VLNIMWWVEE869 pKa = 3.9SCGLIDD875 pKa = 6.11HH876 pKa = 7.2LYY878 pKa = 10.85DD879 pKa = 2.9WTKK882 pKa = 10.18IDD884 pKa = 3.46KK885 pKa = 10.64GGARR889 pKa = 11.84MFHH892 pKa = 7.19MIRR895 pKa = 11.84NAPTPGSKK903 pKa = 9.06SQRR906 pKa = 11.84RR907 pKa = 11.84KK908 pKa = 9.43YY909 pKa = 10.24GKK911 pKa = 9.44EE912 pKa = 3.19AAGYY916 pKa = 10.0EE917 pKa = 4.19DD918 pKa = 3.52VQEE921 pKa = 4.22AVLEE925 pKa = 4.28RR926 pKa = 11.84EE927 pKa = 4.41LQAKK931 pKa = 8.78LLKK934 pKa = 10.3AQRR937 pKa = 11.84LSSAAILNGSPFATPDD953 pKa = 3.27WIARR957 pKa = 11.84NDD959 pKa = 3.54YY960 pKa = 10.53RR961 pKa = 11.84GPNQAQNRR969 pKa = 11.84YY970 pKa = 7.83FQATGEE976 pKa = 4.08EE977 pKa = 4.74PPVKK981 pKa = 8.7MTEE984 pKa = 4.03FLQPGTPSPRR994 pKa = 11.84GAPNIEE1000 pKa = 4.17TIASNIYY1007 pKa = 9.81GLPHH1011 pKa = 6.09QAPAPPEE1018 pKa = 3.62FVEE1021 pKa = 4.72LVKK1024 pKa = 10.53EE1025 pKa = 4.57VYY1027 pKa = 10.45AEE1029 pKa = 3.9NGGRR1033 pKa = 11.84GPNQGQVAKK1042 pKa = 10.82LRR1044 pKa = 11.84MQATLMKK1051 pKa = 9.86GSSPGEE1057 pKa = 3.55TSAAPKK1063 pKa = 9.6PKK1065 pKa = 10.08KK1066 pKa = 10.33KK1067 pKa = 9.42LTTPPKK1073 pKa = 8.34PTSARR1078 pKa = 11.84LGRR1081 pKa = 11.84FMNFGGGLII1090 pKa = 4.05

Molecular weight: 117.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8PKK9|A0A4P8PKK9_9VIRU Structural polyprotein OS=Lates calcarifer birnavirus OX=2583366 PE=4 SV=1
MM1 pKa = 7.72SDD3 pKa = 3.68IFNTAQGRR11 pKa = 11.84SKK13 pKa = 10.57ILASLKK19 pKa = 9.84LQNVTEE25 pKa = 4.19NTQDD29 pKa = 2.85WLLPPRR35 pKa = 11.84WDD37 pKa = 3.93PPADD41 pKa = 3.52TIRR44 pKa = 11.84NSKK47 pKa = 9.14EE48 pKa = 3.18AAEE51 pKa = 3.98ALKK54 pKa = 10.98AGGYY58 pKa = 10.47RR59 pKa = 11.84MLKK62 pKa = 8.99PRR64 pKa = 11.84SIPEE68 pKa = 3.85HH69 pKa = 6.0QPIPTAAALPSLAVLVEE86 pKa = 3.67MDD88 pKa = 4.61AIKK91 pKa = 10.87DD92 pKa = 3.85EE93 pKa = 4.39IEE95 pKa = 4.3LPGGDD100 pKa = 3.66TEE102 pKa = 4.37YY103 pKa = 11.13LPRR106 pKa = 11.84YY107 pKa = 9.38YY108 pKa = 10.05PMHH111 pKa = 6.6KK112 pKa = 9.64PEE114 pKa = 5.14HH115 pKa = 5.95GKK117 pKa = 7.93QTEE120 pKa = 3.88FGMYY124 pKa = 10.15DD125 pKa = 3.82LPLLKK130 pKa = 10.58QMTFQLINGKK140 pKa = 8.45EE141 pKa = 3.94NPAEE145 pKa = 4.01EE146 pKa = 4.55GATFKK151 pKa = 10.74QFRR154 pKa = 11.84DD155 pKa = 3.93TILEE159 pKa = 4.09CQYY162 pKa = 11.72GSGTNAGQIARR173 pKa = 11.84LLAMRR178 pKa = 11.84GVAVGRR184 pKa = 11.84NPNKK188 pKa = 9.74TLAQQGLTLEE198 pKa = 4.16QMAVLLEE205 pKa = 3.94QTLPIGQPGDD215 pKa = 5.06DD216 pKa = 3.62EE217 pKa = 4.89TGWPALTTTLSGLLNPDD234 pKa = 3.27TNEE237 pKa = 4.47DD238 pKa = 3.9YY239 pKa = 11.46LPDD242 pKa = 3.66VTKK245 pKa = 10.58KK246 pKa = 10.58SSAGLPYY253 pKa = 9.88IGKK256 pKa = 8.09TKK258 pKa = 10.61GDD260 pKa = 3.55TMLEE264 pKa = 3.81ALAIGDD270 pKa = 3.69TFLRR274 pKa = 11.84EE275 pKa = 4.02LSAVLSSTTPDD286 pKa = 2.94QKK288 pKa = 11.64DD289 pKa = 3.19RR290 pKa = 11.84FNSLLQDD297 pKa = 3.76FWFLSCGLLFPKK309 pKa = 10.48GEE311 pKa = 4.17RR312 pKa = 11.84YY313 pKa = 10.28DD314 pKa = 3.68RR315 pKa = 11.84DD316 pKa = 2.94AWLTKK321 pKa = 9.28TRR323 pKa = 11.84NIWSAPFPTHH333 pKa = 6.38FLISAISWPIMKK345 pKa = 9.95QSKK348 pKa = 10.66NNTLNMDD355 pKa = 3.79TPSLYY360 pKa = 10.86GFNPFNGGLDD370 pKa = 3.44SIMRR374 pKa = 11.84RR375 pKa = 11.84VEE377 pKa = 3.9KK378 pKa = 11.14GEE380 pKa = 4.08DD381 pKa = 3.07LHH383 pKa = 8.69LIYY386 pKa = 10.68ADD388 pKa = 4.17NIYY391 pKa = 10.4ILQDD395 pKa = 3.95RR396 pKa = 11.84IWFSIDD402 pKa = 3.38LEE404 pKa = 4.37KK405 pKa = 11.61GEE407 pKa = 4.64ANATKK412 pKa = 10.48SHH414 pKa = 6.04AQAIAYY420 pKa = 8.57YY421 pKa = 9.74LLTRR425 pKa = 11.84GWVQDD430 pKa = 3.95DD431 pKa = 4.04GSPAFNATWATLAMQIAPALVVDD454 pKa = 4.67SSCLFMNLQLKK465 pKa = 9.32TYY467 pKa = 9.37GQGSGNPWTFLINHH481 pKa = 7.29ALSTIVVNAWIQAGKK496 pKa = 9.05PRR498 pKa = 11.84PDD500 pKa = 3.42TPQFMALEE508 pKa = 4.33KK509 pKa = 8.71TTGVNFKK516 pKa = 10.14IEE518 pKa = 4.06RR519 pKa = 11.84TIPEE523 pKa = 4.44VPTVALKK530 pKa = 10.81AKK532 pKa = 9.81EE533 pKa = 3.99SSPLVGYY540 pKa = 10.57LGDD543 pKa = 3.8GTNRR547 pKa = 11.84PPEE550 pKa = 4.68KK551 pKa = 10.11EE552 pKa = 4.22APTVDD557 pKa = 4.75LDD559 pKa = 4.14LLGWSATYY567 pKa = 10.73SRR569 pKa = 11.84LLEE572 pKa = 4.04SWVPVLDD579 pKa = 3.8KK580 pKa = 11.35EE581 pKa = 4.99RR582 pKa = 11.84MLKK585 pKa = 9.76SAAYY589 pKa = 9.02PKK591 pKa = 10.63GLEE594 pKa = 4.0NKK596 pKa = 8.45EE597 pKa = 4.41LKK599 pKa = 9.6NQPGAEE605 pKa = 3.85LAYY608 pKa = 9.55TIVRR612 pKa = 11.84NEE614 pKa = 4.08ALRR617 pKa = 11.84MVGGWAYY624 pKa = 10.01PLLDD628 pKa = 3.94RR629 pKa = 11.84SLKK632 pKa = 11.06AMVSAKK638 pKa = 10.32RR639 pKa = 11.84NALTVKK645 pKa = 10.14GIPIEE650 pKa = 4.32SMMGGWQKK658 pKa = 7.99MTEE661 pKa = 3.83FSEE664 pKa = 4.31AFEE667 pKa = 5.43GIDD670 pKa = 3.49ASLEE674 pKa = 4.01VTPEE678 pKa = 3.67FLANMNKK685 pKa = 9.21PRR687 pKa = 11.84GRR689 pKa = 11.84KK690 pKa = 8.19QPHH693 pKa = 4.86VNKK696 pKa = 10.26LALDD700 pKa = 4.12IKK702 pKa = 11.08NMQQASTALTSGAFRR717 pKa = 11.84NPNKK721 pKa = 9.94MAGLKK726 pKa = 9.83LNAMAKK732 pKa = 9.31SKK734 pKa = 10.82LMTTLQAFKK743 pKa = 9.96EE744 pKa = 4.23AEE746 pKa = 3.93AAADD750 pKa = 4.14QSGTDD755 pKa = 3.31DD756 pKa = 3.2WGEE759 pKa = 3.84ASEE762 pKa = 4.67TLDD765 pKa = 3.18TMLRR769 pKa = 11.84ASRR772 pKa = 11.84IYY774 pKa = 9.89QAEE777 pKa = 4.16AEE779 pKa = 4.26ASLKK783 pKa = 10.69DD784 pKa = 3.57VSEE787 pKa = 4.61ALDD790 pKa = 3.66SLSAAAANIKK800 pKa = 7.26TQQEE804 pKa = 4.27KK805 pKa = 8.98DD806 pKa = 2.94TDD808 pKa = 4.24TISNPVVGYY817 pKa = 8.84HH818 pKa = 6.0VPAQRR823 pKa = 11.84SLGVLSSVTGVGPAPVDD840 pKa = 3.54GRR842 pKa = 11.84SKK844 pKa = 10.58NARR847 pKa = 11.84KK848 pKa = 7.96MAKK851 pKa = 9.46RR852 pKa = 11.84RR853 pKa = 11.84SRR855 pKa = 11.84KK856 pKa = 9.35

Molecular weight: 94.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1946

856

1090

973.0

105.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.866 ± 0.11

0.565 ± 0.131

5.19 ± 0.183

5.498 ± 0.28

3.083 ± 0.099

7.451 ± 0.34

1.49 ± 0.267

4.625 ± 0.113

5.653 ± 0.541

9.609 ± 0.835

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.49

4.522 ± 0.163

6.886 ± 0.351

4.06 ± 0.16

4.265 ± 0.177

6.835 ± 0.249

8.171 ± 0.707

5.344 ± 0.764

1.542 ± 0.27

2.878 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski