Enterococcus faecalis (strain ATCC 700802 / V583)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus faecalis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O07109|MURG_ENTFA UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=murG PE=3 SV=2
MM1 pKa = 7.55EE2 pKa = 6.27IIYY5 pKa = 9.69PPLVEE10 pKa = 5.26EE11 pKa = 4.13GLKK14 pKa = 10.76YY15 pKa = 10.8YY16 pKa = 11.02LEE18 pKa = 4.4TTQQSLDD25 pKa = 2.9KK26 pKa = 9.55STFYY30 pKa = 11.11RR31 pKa = 11.84SMVEE35 pKa = 3.61RR36 pKa = 11.84GIITEE41 pKa = 4.3TGLPTQQAIEE51 pKa = 4.06NGLVKK56 pKa = 10.22DD57 pKa = 4.01YY58 pKa = 11.76YY59 pKa = 10.57EE60 pKa = 4.47DD61 pKa = 3.39QGLSFDD67 pKa = 4.33DD68 pKa = 4.14FLRR71 pKa = 11.84IYY73 pKa = 10.13PIFEE77 pKa = 4.38EE78 pKa = 4.29YY79 pKa = 10.61DD80 pKa = 3.48EE81 pKa = 5.71EE82 pKa = 5.1LFQCIDD88 pKa = 4.33GYY90 pKa = 10.24WEE92 pKa = 4.23IPVDD96 pKa = 3.74MKK98 pKa = 11.43EE99 pKa = 4.04NLASQLEE106 pKa = 4.48SGEE109 pKa = 4.19LTFEE113 pKa = 4.87DD114 pKa = 3.78AQQIQAYY121 pKa = 10.47LEE123 pKa = 4.19DD124 pKa = 3.79RR125 pKa = 4.25

Molecular weight: 14.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q82YW0|Q82YW0_ENTFA [Citrate [pro-3S]-lyase] ligase OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=citC PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.33GRR39 pKa = 11.84KK40 pKa = 9.06VISAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3240

0

3240

953506

22

3173

294.3

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.354 ± 0.055

0.663 ± 0.014

4.933 ± 0.04

7.462 ± 0.055

4.598 ± 0.04

6.499 ± 0.045

1.704 ± 0.019

7.237 ± 0.049

7.077 ± 0.045

9.811 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.026

4.704 ± 0.038

3.567 ± 0.032

4.524 ± 0.043

3.74 ± 0.032

5.765 ± 0.038

6.238 ± 0.053

6.917 ± 0.039

0.947 ± 0.017

3.665 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski