Scardovia wiggsiae F0424

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Scardovia; Scardovia wiggsiae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J0DGC0|J0DGC0_9BIFI Aspartate--tRNA(Asp/Asn) ligase OS=Scardovia wiggsiae F0424 OX=857290 GN=aspS PE=3 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.55YY5 pKa = 7.65DD6 pKa = 3.95TPRR9 pKa = 11.84SKK11 pKa = 11.35DD12 pKa = 3.23EE13 pKa = 4.61DD14 pKa = 4.08EE15 pKa = 4.88EE16 pKa = 4.51SLEE19 pKa = 4.08ALSNSTRR26 pKa = 11.84QDD28 pKa = 3.14QASDD32 pKa = 3.86LDD34 pKa = 4.09DD35 pKa = 5.16DD36 pKa = 4.69EE37 pKa = 5.23NAIAEE42 pKa = 4.35DD43 pKa = 4.23YY44 pKa = 9.11EE45 pKa = 5.05LPGADD50 pKa = 4.92LSNEE54 pKa = 4.01DD55 pKa = 3.61SSVTVIPQQGDD66 pKa = 3.42EE67 pKa = 4.67FVCSEE72 pKa = 4.41CFLVYY77 pKa = 10.37NRR79 pKa = 11.84RR80 pKa = 11.84LLDD83 pKa = 3.58HH84 pKa = 6.75YY85 pKa = 11.38EE86 pKa = 3.99NGKK89 pKa = 8.41PVCKK93 pKa = 10.46DD94 pKa = 3.18CAAA97 pKa = 4.2

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J0X0T7|J0X0T7_9BIFI Probable transcriptional regulatory protein HMPREF9156_00203 OS=Scardovia wiggsiae F0424 OX=857290 GN=HMPREF9156_00203 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.62HH16 pKa = 6.11GFRR19 pKa = 11.84QRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84TGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1242

0

1242

455528

29

3236

366.8

40.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.422 ± 0.085

0.863 ± 0.024

6.286 ± 0.061

5.504 ± 0.071

3.452 ± 0.045

8.234 ± 0.064

2.156 ± 0.031

6.035 ± 0.062

4.233 ± 0.061

8.334 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.03

3.416 ± 0.041

4.592 ± 0.037

3.672 ± 0.046

6.101 ± 0.056

7.31 ± 0.07

5.685 ± 0.05

7.072 ± 0.053

1.249 ± 0.027

2.992 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski