Limimonas halophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhodovibrionaceae; Limimonas

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7SQA1|A0A1G7SQA1_9PROT Helicase conserved C-terminal domain-containing protein OS=Limimonas halophila OX=1082479 GN=SAMN05216241_107107 PE=4 SV=1
MM1 pKa = 7.24TMKK4 pKa = 10.52KK5 pKa = 10.09SLYY8 pKa = 9.58ATTALLGVATVANGAHH24 pKa = 6.58AEE26 pKa = 4.53GINLDD31 pKa = 3.33VGGFFNFYY39 pKa = 10.54QSVGDD44 pKa = 3.5PHH46 pKa = 8.45GGFGVDD52 pKa = 3.94DD53 pKa = 3.84TQTFQDD59 pKa = 3.9AEE61 pKa = 4.13ISFSGSYY68 pKa = 9.34TLDD71 pKa = 3.14NGIEE75 pKa = 4.07AGVSITTEE83 pKa = 3.48IANPNSDD90 pKa = 3.35FQQGVEE96 pKa = 3.73ADD98 pKa = 3.55GVYY101 pKa = 10.99GYY103 pKa = 9.8MKK105 pKa = 10.59GSFGTVQLGSQNSAAYY121 pKa = 8.53AQSWGAGPLWSYY133 pKa = 9.46TLVPINTGWQSYY145 pKa = 7.11FTGGTSANAGNFYY158 pKa = 10.99NVGGSTHH165 pKa = 7.21LDD167 pKa = 3.54FANDD171 pKa = 3.58AQRR174 pKa = 11.84IAYY177 pKa = 6.54YY178 pKa = 8.13TPRR181 pKa = 11.84VAGFKK186 pKa = 10.41FGASWAPEE194 pKa = 3.58IQGNNTQQFGFQDD207 pKa = 3.7SDD209 pKa = 3.57TTASDD214 pKa = 3.67GLSASVSWSQDD225 pKa = 3.0FQQVGLSAYY234 pKa = 10.19AGVNTAQAADD244 pKa = 3.68GSGNEE249 pKa = 4.22DD250 pKa = 3.7PEE252 pKa = 5.66HH253 pKa = 7.16YY254 pKa = 9.39MAGVRR259 pKa = 11.84ANFAGFGVGYY269 pKa = 10.82AFAEE273 pKa = 4.33ADD275 pKa = 3.61NPDD278 pKa = 3.95AVSAAQGDD286 pKa = 4.19SPDD289 pKa = 4.02DD290 pKa = 3.8GRR292 pKa = 11.84AHH294 pKa = 6.65GFDD297 pKa = 3.54VVYY300 pKa = 9.71STGPFTFQAEE310 pKa = 4.2ALISRR315 pKa = 11.84TDD317 pKa = 3.62GNSATPDD324 pKa = 3.59ADD326 pKa = 3.3QEE328 pKa = 4.74TYY330 pKa = 10.62KK331 pKa = 11.16GAVGYY336 pKa = 10.51NLGPGINLSGGVIFDD351 pKa = 3.73NQEE354 pKa = 3.49NNAGVDD360 pKa = 3.42SDD362 pKa = 4.28AVSGVIGTTMFFF374 pKa = 3.68

Molecular weight: 38.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7L6K9|A0A1G7L6K9_9PROT Uncharacterized protein OS=Limimonas halophila OX=1082479 GN=SAMN05216241_101140 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.21RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 4.98ANRR9 pKa = 11.84TKK11 pKa = 10.77AHH13 pKa = 6.47RR14 pKa = 11.84LSARR18 pKa = 11.84GHH20 pKa = 6.29RR21 pKa = 11.84KK22 pKa = 6.89QTPPKK27 pKa = 9.8QKK29 pKa = 10.31GQIFFTGGKK38 pKa = 9.6CRR40 pKa = 11.84FF41 pKa = 3.66

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2820

0

2820

886467

28

1626

314.4

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.287 ± 0.082

0.796 ± 0.015

6.261 ± 0.043

6.208 ± 0.04

3.08 ± 0.028

8.931 ± 0.048

2.351 ± 0.024

3.964 ± 0.027

2.49 ± 0.033

9.718 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.02

2.407 ± 0.031

5.328 ± 0.035

3.393 ± 0.031

8.064 ± 0.057

4.538 ± 0.034

5.727 ± 0.038

7.891 ± 0.038

1.252 ± 0.019

2.067 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski