Polaribacter phage P12002L

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Incheonvrus; Polaribacter virus P12002L

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7IJR2|A0A0F7IJR2_9CAUD Terminase small subunit OS=Polaribacter phage P12002L OX=1647386 GN=P12002L_0001 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITRR5 pKa = 11.84EE6 pKa = 3.78LNLTNFEE13 pKa = 4.2FWSGAKK19 pKa = 7.53QHH21 pKa = 6.14YY22 pKa = 7.47FTFSEE27 pKa = 4.15LQEE30 pKa = 3.98IQNQLEE36 pKa = 4.13EE37 pKa = 4.82LYY39 pKa = 9.92PDD41 pKa = 3.86GMSEE45 pKa = 4.22TKK47 pKa = 10.49INDD50 pKa = 3.68LFWFEE55 pKa = 5.02EE56 pKa = 4.34EE57 pKa = 4.72FLCEE61 pKa = 4.46CICLDD66 pKa = 3.78FEE68 pKa = 4.95EE69 pKa = 4.64YY70 pKa = 10.24QNRR73 pKa = 3.65

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7IN68|A0A0F7IN68_9CAUD Uncharacterized protein OS=Polaribacter phage P12002L OX=1647386 GN=P12002L_0061 PE=4 SV=1
MM1 pKa = 7.79HH2 pKa = 7.64EE3 pKa = 4.65LLKK6 pKa = 10.76LYY8 pKa = 9.25QNRR11 pKa = 11.84VKK13 pKa = 10.46PVQFLLNKK21 pKa = 5.59TTNRR25 pKa = 11.84EE26 pKa = 3.39RR27 pKa = 11.84RR28 pKa = 11.84IEE30 pKa = 3.96LNEE33 pKa = 3.78RR34 pKa = 11.84LQEE37 pKa = 3.86IARR40 pKa = 11.84AIYY43 pKa = 9.32EE44 pKa = 3.98IKK46 pKa = 10.62KK47 pKa = 10.62RR48 pKa = 11.84MM49 pKa = 3.45

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14569

39

1124

177.7

20.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.386 ± 0.395

0.94 ± 0.151

6.013 ± 0.226

7.64 ± 0.323

4.407 ± 0.241

5.525 ± 0.416

1.311 ± 0.176

7.893 ± 0.257

11.133 ± 0.659

8.82 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.151

7.365 ± 0.279

2.231 ± 0.193

3.151 ± 0.185

3.734 ± 0.19

6.431 ± 0.329

5.731 ± 0.369

5.635 ± 0.225

1.098 ± 0.098

4.475 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski