Micromonospora pattaloongensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3HWF0|A0A1H3HWF0_9ACTN RNA polymerase sigma subunit ECF family OS=Micromonospora pattaloongensis OX=405436 GN=SAMN05444365_101995 PE=3 SV=1
MM1 pKa = 7.53SSYY4 pKa = 10.78GPPGAPGQGQPDD16 pKa = 3.99EE17 pKa = 5.4PYY19 pKa = 10.12GQPPQPPAPPPPGEE33 pKa = 4.29YY34 pKa = 9.59PPPPAYY40 pKa = 9.17TPPPAPGAPAGSPPPAGAFPPPPPGAFPPPPPGGFPPPPQGGFPAPDD87 pKa = 3.42YY88 pKa = 7.82PAPSSGAPAWGQPPAPGMPVPPPASSKK115 pKa = 10.45RR116 pKa = 11.84GLKK119 pKa = 9.75IGLIIGAVVLLLLCLCGGLFVYY141 pKa = 10.42AVANYY146 pKa = 9.96EE147 pKa = 4.0DD148 pKa = 4.51DD149 pKa = 4.41AADD152 pKa = 3.5SVAAPTASASPSEE165 pKa = 4.51SPSVSPSPSEE175 pKa = 5.67DD176 pKa = 3.18DD177 pKa = 3.68TADD180 pKa = 4.18DD181 pKa = 4.78DD182 pKa = 5.08LVKK185 pKa = 10.74GDD187 pKa = 4.01CVVNEE192 pKa = 4.45GTDD195 pKa = 3.11SDD197 pKa = 4.24AEE199 pKa = 4.13LRR201 pKa = 11.84KK202 pKa = 10.08VPCGPDD208 pKa = 2.93TFEE211 pKa = 5.01ILAKK215 pKa = 10.31IPFTTDD221 pKa = 2.26SKK223 pKa = 10.09RR224 pKa = 11.84CEE226 pKa = 4.03NQFMGSPEE234 pKa = 4.09TDD236 pKa = 2.96TTFTSDD242 pKa = 4.29GPGSLNDD249 pKa = 4.05FVLCMKK255 pKa = 10.31EE256 pKa = 3.65RR257 pKa = 3.84

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3GYR4|A0A1H3GYR4_9ACTN Polyphosphate:nucleotide phosphotransferase PPK2 family OS=Micromonospora pattaloongensis OX=405436 GN=SAMN05444365_101613 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4874

0

4874

1584710

26

2602

325.1

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.376 ± 0.063

0.752 ± 0.01

5.885 ± 0.027

5.13 ± 0.035

2.67 ± 0.022

9.327 ± 0.031

2.068 ± 0.015

3.332 ± 0.022

1.724 ± 0.025

10.296 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.012

1.84 ± 0.024

6.41 ± 0.04

2.722 ± 0.02

8.547 ± 0.04

4.635 ± 0.03

6.043 ± 0.033

8.966 ± 0.034

1.519 ± 0.016

2.086 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski