Bacillus phage Ray17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386K728|A0A386K728_9CAUD Tape measure protein OS=Bacillus phage Ray17 OX=2315627 GN=Ray17_58 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 9.88FYY4 pKa = 10.45EE5 pKa = 4.57IRR7 pKa = 11.84DD8 pKa = 3.65PYY10 pKa = 9.99YY11 pKa = 11.33ALIKK15 pKa = 10.91AEE17 pKa = 4.57DD18 pKa = 3.51KK19 pKa = 11.56AEE21 pKa = 4.01AEE23 pKa = 4.08KK24 pKa = 11.06VYY26 pKa = 11.27NEE28 pKa = 3.83MVAYY32 pKa = 7.08TTGEE36 pKa = 3.94DD37 pKa = 3.68EE38 pKa = 5.67IEE40 pKa = 4.6DD41 pKa = 3.96FQEE44 pKa = 4.74DD45 pKa = 4.16EE46 pKa = 4.18IKK48 pKa = 10.65EE49 pKa = 4.11VSRR52 pKa = 11.84DD53 pKa = 3.38YY54 pKa = 11.78ALVIFSQIQDD64 pKa = 3.34HH65 pKa = 7.36DD66 pKa = 4.51GTCAGYY72 pKa = 10.87DD73 pKa = 3.75FIKK76 pKa = 11.01GMFNDD81 pKa = 4.46PDD83 pKa = 3.57IKK85 pKa = 10.38LLKK88 pKa = 9.61MDD90 pKa = 4.59GSLLL94 pKa = 3.84

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386K9G5|A0A386K9G5_9CAUD Uncharacterized protein OS=Bacillus phage Ray17 OX=2315627 GN=Ray17_5 PE=4 SV=1
MM1 pKa = 7.29TKK3 pKa = 10.39VITVINFCDD12 pKa = 3.11SRR14 pKa = 11.84YY15 pKa = 8.84VQKK18 pKa = 10.66KK19 pKa = 7.23RR20 pKa = 11.84QKK22 pKa = 8.78KK23 pKa = 7.65RR24 pKa = 11.84QRR26 pKa = 11.84KK27 pKa = 8.14RR28 pKa = 11.84QIKK31 pKa = 9.86DD32 pKa = 2.84AANPLLPRR40 pKa = 11.84DD41 pKa = 3.54SGFWEE46 pKa = 4.25DD47 pKa = 4.88TKK49 pKa = 9.51TTDD52 pKa = 4.81KK53 pKa = 10.3NTQKK57 pKa = 9.14EE58 pKa = 4.47TQKK61 pKa = 10.57RR62 pKa = 11.84QRR64 pKa = 11.84NDD66 pKa = 2.75TKK68 pKa = 9.91QTMRR72 pKa = 11.84TMINKK77 pKa = 9.25

Molecular weight: 9.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13521

45

1084

182.7

20.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.506

0.784 ± 0.139

6.516 ± 0.423

7.958 ± 0.581

3.927 ± 0.167

7.122 ± 0.345

1.59 ± 0.15

6.679 ± 0.277

8.809 ± 0.381

7.1 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.143 ± 0.227

5.17 ± 0.201

3.032 ± 0.201

3.336 ± 0.179

4.896 ± 0.286

5.68 ± 0.341

5.747 ± 0.31

6.427 ± 0.293

1.464 ± 0.162

3.654 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski