Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; Nitrosomonas europaea

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q82Y80|Q82Y80_NITEU Possible 16S pseudouridylate synthase OS=Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) OX=228410 GN=NE0005 PE=4 SV=1
MM1 pKa = 7.41TYY3 pKa = 10.69VVTEE7 pKa = 3.93SCIKK11 pKa = 10.42CKK13 pKa = 10.15YY14 pKa = 7.55TDD16 pKa = 4.08CVDD19 pKa = 3.61VCPVDD24 pKa = 4.18CFRR27 pKa = 11.84EE28 pKa = 4.9GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.64EE39 pKa = 6.0CIDD42 pKa = 3.74CTLCVAEE49 pKa = 5.08CPVEE53 pKa = 4.68AIYY56 pKa = 11.14AEE58 pKa = 4.52DD59 pKa = 5.46DD60 pKa = 3.64VPEE63 pKa = 4.42DD64 pKa = 3.29QRR66 pKa = 11.84QFIALNAEE74 pKa = 4.39LSKK77 pKa = 10.46IWDD80 pKa = 4.35PIIEE84 pKa = 4.73KK85 pKa = 10.42KK86 pKa = 10.48DD87 pKa = 3.57ALPDD91 pKa = 3.79ADD93 pKa = 3.54EE94 pKa = 4.35WASVTDD100 pKa = 4.47KK101 pKa = 11.13LDD103 pKa = 3.33KK104 pKa = 11.14LEE106 pKa = 4.03RR107 pKa = 5.16

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q82X99|RNPA_NITEU Ribonuclease P protein component OS=Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) OX=228410 GN=rnpA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.69QPSVISRR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 6.19GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84AKK41 pKa = 10.75LSVV44 pKa = 3.1

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2375

0

2375

777646

42

3064

327.4

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.28 ± 0.053

0.959 ± 0.018

5.354 ± 0.043

6.004 ± 0.048

3.928 ± 0.036

7.205 ± 0.04

2.494 ± 0.023

6.417 ± 0.042

4.059 ± 0.044

10.706 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.025

3.56 ± 0.036

4.593 ± 0.03

4.307 ± 0.034

6.364 ± 0.043

6.013 ± 0.033

5.461 ± 0.058

6.714 ± 0.047

1.323 ± 0.024

2.813 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski