Bacteroides fluxus YIT 12057

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides fluxus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F3PQ80|F3PQ80_9BACE OmpR/PhoB-type domain-containing protein OS=Bacteroides fluxus YIT 12057 OX=763034 GN=HMPREF9446_00872 PE=4 SV=1
MM1 pKa = 7.96KK2 pKa = 9.31ITTEE6 pKa = 3.66MARR9 pKa = 11.84YY10 pKa = 7.8IWSIIAANPSLSMSWGVDD28 pKa = 2.69TDD30 pKa = 4.32SFVCSDD36 pKa = 4.12DD37 pKa = 3.84ALEE40 pKa = 3.87FHH42 pKa = 6.81IQGFLHH48 pKa = 6.65IGNVRR53 pKa = 11.84VTYY56 pKa = 10.44IEE58 pKa = 4.4GVDD61 pKa = 3.7LFQVSLYY68 pKa = 10.63DD69 pKa = 3.64EE70 pKa = 4.95EE71 pKa = 5.2GNEE74 pKa = 4.24VKK76 pKa = 10.63RR77 pKa = 11.84IDD79 pKa = 5.6DD80 pKa = 3.88VYY82 pKa = 11.25LDD84 pKa = 3.97NLAEE88 pKa = 4.45VIDD91 pKa = 4.15QNVEE95 pKa = 3.7NYY97 pKa = 10.21LVEE100 pKa = 5.04DD101 pKa = 3.93YY102 pKa = 10.55NAKK105 pKa = 9.24VMNYY109 pKa = 9.84ILGLL113 pKa = 3.77

Molecular weight:
12.93 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F3PRE3|F3PRE3_9BACE Replicative DNA helicase OS=Bacteroides fluxus YIT 12057 OX=763034 GN=HMPREF9446_01293 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.56GNSHH6 pKa = 6.64SNARR10 pKa = 11.84PKK12 pKa = 10.32RR13 pKa = 11.84ANKK16 pKa = 9.25TKK18 pKa = 8.67TTGKK22 pKa = 10.37RR23 pKa = 11.84IGKK26 pKa = 9.06SKK28 pKa = 10.31PSKK31 pKa = 10.93ANNQLNRR38 pKa = 11.84RR39 pKa = 11.84VKK41 pKa = 10.41

Molecular weight:
4.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3920

0

3920

1321397

35

2040

337.1

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.267 ± 0.04

1.302 ± 0.015

5.416 ± 0.027

6.6 ± 0.036

4.584 ± 0.026

6.956 ± 0.035

1.913 ± 0.019

6.706 ± 0.038

6.55 ± 0.037

9.212 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.018

4.964 ± 0.037

3.772 ± 0.022

3.407 ± 0.019

4.844 ± 0.031

5.995 ± 0.034

5.479 ± 0.028

6.523 ± 0.03

1.263 ± 0.015

4.432 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski