Cyclovirus PK5222

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Human associated cyclovirus 3

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4N3P1|D4N3P1_9CIRC Capsid protein OS=Cyclovirus PK5222 OX=742917 GN=cap PE=4 SV=1
MM1 pKa = 7.79ANRR4 pKa = 11.84TVRR7 pKa = 11.84RR8 pKa = 11.84FCFTWNNYY16 pKa = 9.07PDD18 pKa = 3.55TAYY21 pKa = 9.71EE22 pKa = 3.9KK23 pKa = 11.16CEE25 pKa = 3.72TFIQKK30 pKa = 9.39FCKK33 pKa = 9.87YY34 pKa = 9.77GIVGEE39 pKa = 4.32EE40 pKa = 3.99LAPTTGTPHH49 pKa = 6.72LQGFCNLRR57 pKa = 11.84KK58 pKa = 8.07PTRR61 pKa = 11.84FSTIKK66 pKa = 10.1KK67 pKa = 9.39HH68 pKa = 6.26LDD70 pKa = 3.16NSIHH74 pKa = 6.21IEE76 pKa = 3.91KK77 pKa = 10.91ANGSDD82 pKa = 3.6EE83 pKa = 4.27QNQAYY88 pKa = 9.81CSKK91 pKa = 9.6TGIFFEE97 pKa = 4.77EE98 pKa = 5.04GSPTKK103 pKa = 10.28QGQRR107 pKa = 11.84NDD109 pKa = 3.5LQLLVDD115 pKa = 4.78DD116 pKa = 5.49VIGHH120 pKa = 6.92PDD122 pKa = 3.38IKK124 pKa = 10.25TKK126 pKa = 10.64DD127 pKa = 3.14IAAKK131 pKa = 10.19YY132 pKa = 8.33PSTFVRR138 pKa = 11.84YY139 pKa = 8.67FRR141 pKa = 11.84GIKK144 pKa = 8.52EE145 pKa = 4.27LQRR148 pKa = 11.84VVHH151 pKa = 6.27PVAEE155 pKa = 4.28RR156 pKa = 11.84MFKK159 pKa = 10.23TEE161 pKa = 3.5VYY163 pKa = 9.89YY164 pKa = 10.94YY165 pKa = 9.29WGPPGTGKK173 pKa = 10.0SRR175 pKa = 11.84RR176 pKa = 11.84ALEE179 pKa = 3.95EE180 pKa = 3.76AQATGNGSIYY190 pKa = 10.21YY191 pKa = 9.83KK192 pKa = 10.55PRR194 pKa = 11.84GLWWDD199 pKa = 3.85GYY201 pKa = 7.45EE202 pKa = 4.15QQDD205 pKa = 4.01SVIIDD210 pKa = 4.35DD211 pKa = 4.43FYY213 pKa = 11.87GWIKK217 pKa = 10.75YY218 pKa = 10.28DD219 pKa = 3.96EE220 pKa = 4.46LLKK223 pKa = 10.08ITDD226 pKa = 4.0RR227 pKa = 11.84YY228 pKa = 8.81PYY230 pKa = 10.05KK231 pKa = 10.76VQIKK235 pKa = 10.43GGFEE239 pKa = 3.86EE240 pKa = 4.76FTSKK244 pKa = 10.84KK245 pKa = 10.28IFITSNVDD253 pKa = 2.68TDD255 pKa = 3.58EE256 pKa = 4.68LYY258 pKa = 10.98KK259 pKa = 11.1FNGYY263 pKa = 7.66NTAALEE269 pKa = 3.92RR270 pKa = 11.84RR271 pKa = 11.84LTVKK275 pKa = 10.48EE276 pKa = 4.03YY277 pKa = 10.32MSS279 pKa = 3.71

Molecular weight: 32.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4N3P1|D4N3P1_9CIRC Capsid protein OS=Cyclovirus PK5222 OX=742917 GN=cap PE=4 SV=1
MM1 pKa = 7.7ALRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.67RR7 pKa = 11.84RR8 pKa = 11.84VRR10 pKa = 11.84RR11 pKa = 11.84PLRR14 pKa = 11.84RR15 pKa = 11.84YY16 pKa = 8.56RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 8.86FFRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.74SRR29 pKa = 11.84VGKK32 pKa = 10.37SDD34 pKa = 4.48LLVKK38 pKa = 9.39LTKK41 pKa = 9.73IDD43 pKa = 3.69TVNVDD48 pKa = 2.77ISKK51 pKa = 10.52INVWSLSYY59 pKa = 10.93LPTDD63 pKa = 4.0FSEE66 pKa = 4.34YY67 pKa = 11.15NEE69 pKa = 3.92LKK71 pKa = 10.9KK72 pKa = 10.83NFEE75 pKa = 4.11YY76 pKa = 10.77CVFLKK81 pKa = 10.51EE82 pKa = 4.14RR83 pKa = 11.84VTIYY87 pKa = 10.62PMQNIANNSTSQVPAYY103 pKa = 10.93LMAPWHH109 pKa = 6.44RR110 pKa = 11.84GGPASATFNTYY121 pKa = 8.54LTIDD125 pKa = 3.33RR126 pKa = 11.84AKK128 pKa = 10.18IFRR131 pKa = 11.84GTQVGSQTYY140 pKa = 8.66VPSVLIEE147 pKa = 4.09GLSDD151 pKa = 3.47SGGGTSSPDD160 pKa = 3.16MIQFRR165 pKa = 11.84PKK167 pKa = 10.41VYY169 pKa = 10.16ISNSQTRR176 pKa = 11.84AIRR179 pKa = 11.84IYY181 pKa = 10.5NGIIAFQGNGEE192 pKa = 4.17MTGTARR198 pKa = 11.84YY199 pKa = 8.6NVKK202 pKa = 10.08HH203 pKa = 6.07DD204 pKa = 3.18VWVVFKK210 pKa = 10.83KK211 pKa = 10.75QNTLASLL218 pKa = 3.83

Molecular weight: 25.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

497

218

279

248.5

28.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.829 ± 0.132

1.207 ± 0.455

4.829 ± 0.704

5.231 ± 1.505

5.231 ± 0.391

6.841 ± 0.533

1.408 ± 0.298

6.439 ± 0.01

7.646 ± 0.743

6.036 ± 0.513

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.61 ± 0.415

5.03 ± 0.567

4.225 ± 0.059

4.024 ± 0.215

8.048 ± 1.798

6.036 ± 1.348

7.445 ± 0.342

6.036 ± 1.07

1.61 ± 0.142

6.237 ± 0.445
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski