Beihai picorna-like virus 120

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHP5|A0A1L3KHP5_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 120 OX=1922549 PE=4 SV=1
MM1 pKa = 6.96STRR4 pKa = 11.84AAPATASEE12 pKa = 4.78GVTDD16 pKa = 3.73QHH18 pKa = 6.64PSEE21 pKa = 4.64SGYY24 pKa = 10.7VPNAMGNPAYY34 pKa = 9.4PAIPTRR40 pKa = 11.84TDD42 pKa = 3.56EE43 pKa = 3.85IPEE46 pKa = 4.07RR47 pKa = 11.84VKK49 pKa = 10.82ALNMLDD55 pKa = 3.62PYY57 pKa = 10.68FYY59 pKa = 11.18SQFVLLNSFSWSNQDD74 pKa = 3.5QKK76 pKa = 11.98NKK78 pKa = 9.98ILWSIEE84 pKa = 3.72LKK86 pKa = 10.18PEE88 pKa = 3.86NMHH91 pKa = 7.24ANLAYY96 pKa = 8.44ITAMYY101 pKa = 8.83NAWVGDD107 pKa = 3.83FEE109 pKa = 5.35FMLKK113 pKa = 9.89IAGTGFHH120 pKa = 6.75GGALGVAWIPPNLNVEE136 pKa = 4.4DD137 pKa = 4.48LSGAEE142 pKa = 4.05LTIFDD147 pKa = 4.95YY148 pKa = 11.51LLLDD152 pKa = 4.98PKK154 pKa = 10.52MQDD157 pKa = 3.05LKK159 pKa = 11.4GFAGKK164 pKa = 9.6DD165 pKa = 3.26VKK167 pKa = 11.16NVDD170 pKa = 4.31FHH172 pKa = 6.63WKK174 pKa = 10.19DD175 pKa = 3.33NEE177 pKa = 3.83AAAQFNSDD185 pKa = 3.58FAKK188 pKa = 10.06VASKK192 pKa = 10.9GGYY195 pKa = 9.17LVMFVVLPLITSTTGNNAINLAIFNRR221 pKa = 11.84VAPTFRR227 pKa = 11.84VGQMIPIKK235 pKa = 9.79YY236 pKa = 8.99VKK238 pKa = 9.9PSPALFHH245 pKa = 7.68DD246 pKa = 4.57PFLDD250 pKa = 4.15FSMGVNLRR258 pKa = 11.84NYY260 pKa = 10.08INFGFFKK267 pKa = 10.23QIRR270 pKa = 11.84IEE272 pKa = 4.05PVTIMTTDD280 pKa = 3.09QFRR283 pKa = 11.84DD284 pKa = 3.35LNLSFKK290 pKa = 11.27NMTIVDD296 pKa = 4.28RR297 pKa = 11.84SATSIRR303 pKa = 11.84QVTDD307 pKa = 2.85NGDD310 pKa = 2.9ISFTYY315 pKa = 10.01VRR317 pKa = 11.84SNGVPNPEE325 pKa = 4.88KK326 pKa = 9.81IAKK329 pKa = 10.04GITASQNLVAVEE341 pKa = 4.31WKK343 pKa = 10.69SSANDD348 pKa = 3.51YY349 pKa = 10.35EE350 pKa = 4.8VEE352 pKa = 4.24VANVAGGRR360 pKa = 11.84IGQVHH365 pKa = 7.03DD366 pKa = 3.9AVITYY371 pKa = 8.92GALYY375 pKa = 10.41QSDD378 pKa = 4.27ASWFPNFTNSGGFLTITNGLDD399 pKa = 3.05EE400 pKa = 4.9SLVTFCGQSASNAGFTLPGTVVEE423 pKa = 4.12MLQRR427 pKa = 11.84GMFKK431 pKa = 10.63GIPDD435 pKa = 4.25GQDD438 pKa = 2.72VVYY441 pKa = 10.42DD442 pKa = 3.61IVDD445 pKa = 3.39IPTDD449 pKa = 3.39LVTRR453 pKa = 11.84QIRR456 pKa = 11.84LTEE459 pKa = 3.75IDD461 pKa = 3.36GSVFFTTNKK470 pKa = 8.5TDD472 pKa = 3.3QPIILEE478 pKa = 4.04ASKK481 pKa = 11.19SKK483 pKa = 10.59FVFSEE488 pKa = 4.65FIGRR492 pKa = 11.84EE493 pKa = 4.04TPLTAPAAEE502 pKa = 4.27MKK504 pKa = 10.48RR505 pKa = 11.84NLEE508 pKa = 3.73IVLQRR513 pKa = 11.84DD514 pKa = 3.2RR515 pKa = 11.84LAKK518 pKa = 9.99IEE520 pKa = 3.94QAMAEE525 pKa = 4.25SQMM528 pKa = 3.85

Molecular weight: 58.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHP1|A0A1L3KHP1_9VIRU Calici_coat domain-containing protein OS=Beihai picorna-like virus 120 OX=1922549 PE=4 SV=1
MM1 pKa = 7.32AAVAADD7 pKa = 3.86AAAGEE12 pKa = 4.3ASAGAEE18 pKa = 4.19GGSSGISQGASNIAGGLMKK37 pKa = 10.57GAFGLIGQRR46 pKa = 11.84VGADD50 pKa = 3.15LQLRR54 pKa = 11.84NFKK57 pKa = 11.18AEE59 pKa = 3.81GDD61 pKa = 3.82YY62 pKa = 11.0LQNSFQSAGLPGVLAFANGGSSLPIMNQRR91 pKa = 11.84IGQTGLNTLQINSFTPYY108 pKa = 10.16TGTLAQNMFHH118 pKa = 6.97VGKK121 pKa = 10.33LWW123 pKa = 3.28

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3001

123

2178

750.3

84.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.864 ± 1.444

1.766 ± 0.681

6.165 ± 0.564

6.265 ± 1.163

4.798 ± 0.572

6.331 ± 1.611

2.299 ± 0.575

5.398 ± 0.706

6.564 ± 1.014

8.031 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.099

4.432 ± 0.86

4.765 ± 0.313

4.532 ± 0.334

4.732 ± 0.835

6.165 ± 1.84

5.831 ± 0.338

7.164 ± 0.478

1.433 ± 0.093

3.332 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski