Polaribacter phage P12002S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Incheonvrus; Polaribacter virus P12002S

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7IN77|A0A0F7IN77_9CAUD Uncharacterized protein OS=Polaribacter phage P12002S OX=1647387 GN=P12002S_0004 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITRR5 pKa = 11.84EE6 pKa = 3.78LNLTNFEE13 pKa = 4.2FWSGAKK19 pKa = 7.53QHH21 pKa = 6.14YY22 pKa = 7.47FTFSEE27 pKa = 4.15LQEE30 pKa = 3.98IQNQLEE36 pKa = 4.13EE37 pKa = 4.82LYY39 pKa = 9.92PDD41 pKa = 3.86GMSEE45 pKa = 4.22TKK47 pKa = 10.49INDD50 pKa = 3.68LFWFEE55 pKa = 5.02EE56 pKa = 4.34EE57 pKa = 4.72FLCEE61 pKa = 4.46CICLDD66 pKa = 3.78FEE68 pKa = 4.95EE69 pKa = 4.64YY70 pKa = 10.24QNRR73 pKa = 3.65

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7DD21|A0A0F7DD21_9CAUD Uncharacterized protein OS=Polaribacter phage P12002S OX=1647387 GN=P12002S_0010 PE=4 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.02KK4 pKa = 10.96TLIKK8 pKa = 10.73YY9 pKa = 10.19KK10 pKa = 10.46ILLKK14 pKa = 10.55EE15 pKa = 3.91LGYY18 pKa = 9.35TGKK21 pKa = 10.58DD22 pKa = 3.31MANMLGIKK30 pKa = 9.41YY31 pKa = 10.1SSYY34 pKa = 10.96RR35 pKa = 11.84SMTRR39 pKa = 11.84KK40 pKa = 8.31TNRR43 pKa = 11.84TLPKK47 pKa = 7.87WVKK50 pKa = 9.82IFVIVHH56 pKa = 6.51EE57 pKa = 4.09ILEE60 pKa = 4.41RR61 pKa = 11.84KK62 pKa = 8.96HH63 pKa = 6.33RR64 pKa = 11.84KK65 pKa = 9.47QIDD68 pKa = 3.35KK69 pKa = 11.33YY70 pKa = 10.05EE71 pKa = 4.14NKK73 pKa = 9.99TNN75 pKa = 3.53

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

14839

37

1273

172.5

19.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.502 ± 0.425

1.004 ± 0.139

6.132 ± 0.214

7.743 ± 0.255

4.428 ± 0.185

5.425 ± 0.332

1.26 ± 0.151

7.837 ± 0.218

11.025 ± 0.655

8.754 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.13

7.204 ± 0.328

2.217 ± 0.186

3.147 ± 0.17

3.639 ± 0.185

6.234 ± 0.309

6.213 ± 0.34

5.593 ± 0.205

1.085 ± 0.093

4.475 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski