Sterolibacterium denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sterolibacteriaceae; Sterolibacterium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A656Z7R7|A0A656Z7R7_9PROT Uncharacterized protein OS=Sterolibacterium denitrificans OX=157592 GN=ACY05_00550 PE=4 SV=1
MM1 pKa = 8.07SLMITDD7 pKa = 3.76EE8 pKa = 4.96CINCDD13 pKa = 3.27VCEE16 pKa = 4.27PEE18 pKa = 4.83CPNNAISQGEE28 pKa = 4.52EE29 pKa = 3.71IYY31 pKa = 10.84EE32 pKa = 3.96IDD34 pKa = 3.64PNKK37 pKa = 9.63CTEE40 pKa = 4.34CVGHH44 pKa = 7.03FDD46 pKa = 3.71TPQCRR51 pKa = 11.84EE52 pKa = 4.06VCPVDD57 pKa = 5.77CIPNDD62 pKa = 3.75PKK64 pKa = 10.88HH65 pKa = 6.79VEE67 pKa = 3.99NQEE70 pKa = 3.74QLMDD74 pKa = 3.83KK75 pKa = 10.25FLKK78 pKa = 9.54LTADD82 pKa = 3.59KK83 pKa = 11.28

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A656Z8Z9|A0A656Z8Z9_9PROT Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Sterolibacterium denitrificans OX=157592 GN=purC PE=3 SV=1
MM1 pKa = 7.91GNFTWLDD8 pKa = 3.71LLILAVILFSSLLGVLRR25 pKa = 11.84GMLSEE30 pKa = 4.25VLALLAWVAGFIAANAWAGTAAGLLDD56 pKa = 3.66GMLRR60 pKa = 11.84VPGDD64 pKa = 3.14PSLRR68 pKa = 11.84HH69 pKa = 4.93IVGFVTVFALVLISFAIARR88 pKa = 11.84RR89 pKa = 11.84LLASLLHH96 pKa = 6.19AVGLGPLDD104 pKa = 3.79RR105 pKa = 11.84LLGAVFGIARR115 pKa = 11.84GVLVVWTGVLICGLTEE131 pKa = 5.29LPRR134 pKa = 11.84QPWWHH139 pKa = 6.22EE140 pKa = 3.74AQLIPPLEE148 pKa = 4.15TAVIASKK155 pKa = 10.0PWLPLALAKK164 pKa = 10.28KK165 pKa = 9.57IRR167 pKa = 11.84YY168 pKa = 9.04RR169 pKa = 11.84SAAAAAA175 pKa = 4.03

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

780

0

780

265175

57

2103

340.0

37.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.565 ± 0.142

0.976 ± 0.032

5.511 ± 0.089

5.898 ± 0.062

3.384 ± 0.059

8.141 ± 0.083

2.271 ± 0.044

5.116 ± 0.063

3.337 ± 0.066

10.964 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.045

2.924 ± 0.06

4.94 ± 0.06

4.149 ± 0.066

6.969 ± 0.081

5.188 ± 0.065

4.922 ± 0.073

6.683 ± 0.08

1.277 ± 0.033

2.349 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski