Balneola sp.

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Balneola; unclassified Balneola

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8G177|A0A3M8G177_9BACT Four helix bundle protein OS=Balneola sp. OX=2024824 GN=ED557_09380 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.28KK3 pKa = 10.57LILIPVLIIIAFISCKK19 pKa = 10.32DD20 pKa = 3.53NPAGISGEE28 pKa = 4.11YY29 pKa = 10.85GEE31 pKa = 4.45VNSWIRR37 pKa = 11.84TQMDD41 pKa = 4.61FYY43 pKa = 11.02YY44 pKa = 10.54FWDD47 pKa = 3.53QYY49 pKa = 11.78VPDD52 pKa = 4.21EE53 pKa = 4.68ADD55 pKa = 3.19GTVAPEE61 pKa = 3.74VFYY64 pKa = 11.13EE65 pKa = 5.0SIQEE69 pKa = 4.05PNDD72 pKa = 3.44RR73 pKa = 11.84FSFISDD79 pKa = 3.45DD80 pKa = 3.87AQALLDD86 pKa = 4.42DD87 pKa = 4.85LSGNSVSAGYY97 pKa = 10.24SPAFGRR103 pKa = 11.84ISSTDD108 pKa = 3.03EE109 pKa = 3.59VFIVVEE115 pKa = 4.86FVYY118 pKa = 10.32PGTPAADD125 pKa = 3.33GGMEE129 pKa = 4.28RR130 pKa = 11.84GDD132 pKa = 4.14IILGINGIRR141 pKa = 11.84LDD143 pKa = 3.74TLNYY147 pKa = 10.28LDD149 pKa = 4.9LFYY152 pKa = 10.92TEE154 pKa = 4.95GMSTFTMGEE163 pKa = 3.95ANFSEE168 pKa = 4.53EE169 pKa = 3.94EE170 pKa = 3.52QRR172 pKa = 11.84YY173 pKa = 9.18ILTEE177 pKa = 3.76TGEE180 pKa = 4.44TITADD185 pKa = 3.15VGQIEE190 pKa = 5.32LDD192 pKa = 3.57PVVYY196 pKa = 9.93TSIIDD201 pKa = 3.75TSAHH205 pKa = 6.09KK206 pKa = 9.84IGYY209 pKa = 7.7MFYY212 pKa = 10.87SRR214 pKa = 11.84FVDD217 pKa = 3.47GDD219 pKa = 3.28EE220 pKa = 5.38DD221 pKa = 4.08IFVTSLINTLTDD233 pKa = 3.74FNNQGVTEE241 pKa = 4.16LVVDD245 pKa = 4.13LRR247 pKa = 11.84YY248 pKa = 10.58NPGGQVSVATEE259 pKa = 3.9FANALVPSINAQNEE273 pKa = 4.52DD274 pKa = 2.78IFIQYY279 pKa = 9.41EE280 pKa = 4.18YY281 pKa = 11.37NEE283 pKa = 4.85EE284 pKa = 3.96YY285 pKa = 10.73TNALIDD291 pKa = 3.8QQGLDD296 pKa = 4.08SPNLSTRR303 pKa = 11.84FSEE306 pKa = 4.9GDD308 pKa = 3.32VNLNLDD314 pKa = 3.32KK315 pKa = 11.26VYY317 pKa = 10.84FLVTGASASASEE329 pKa = 4.78LIVTGLEE336 pKa = 4.25PYY338 pKa = 9.67MDD340 pKa = 4.26VYY342 pKa = 11.07TIGTPTVGKK351 pKa = 10.08FYY353 pKa = 10.96GAFVLADD360 pKa = 3.74DD361 pKa = 4.12VSNYY365 pKa = 10.67AIVPVSFKK373 pKa = 10.9YY374 pKa = 10.87LNADD378 pKa = 3.53GFTDD382 pKa = 4.81FASGLDD388 pKa = 3.56PDD390 pKa = 4.95FSTFEE395 pKa = 4.3SLFATVPIGDD405 pKa = 4.44LNDD408 pKa = 4.18PLFSTAIEE416 pKa = 4.56HH417 pKa = 6.15IVNGSVVTKK426 pKa = 8.78PLPFTRR432 pKa = 11.84EE433 pKa = 4.43FIPLRR438 pKa = 11.84DD439 pKa = 4.31PIEE442 pKa = 4.24LKK444 pKa = 10.77NGNVLLDD451 pKa = 3.92FPEE454 pKa = 4.26EE455 pKa = 4.02FF456 pKa = 4.27

Molecular weight: 50.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8FQ57|A0A3M8FQ57_9BACT Sensor histidine kinase OS=Balneola sp. OX=2024824 GN=ED557_14720 PE=4 SV=1
MM1 pKa = 7.57AHH3 pKa = 7.61PKK5 pKa = 10.33RR6 pKa = 11.84KK7 pKa = 7.54TSKK10 pKa = 9.88ARR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 3.43KK15 pKa = 10.9RR16 pKa = 11.84RR17 pKa = 11.84SHH19 pKa = 6.51HH20 pKa = 6.92AIADD24 pKa = 3.6VTLAKK29 pKa = 10.41CSNCGTLHH37 pKa = 7.19RR38 pKa = 11.84YY39 pKa = 7.94HH40 pKa = 6.85HH41 pKa = 7.03ACPEE45 pKa = 3.84CGYY48 pKa = 10.54YY49 pKa = 10.03RR50 pKa = 11.84GRR52 pKa = 11.84QVISVANN59 pKa = 3.74

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3130

0

3130

1114347

22

3034

356.0

39.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.472 ± 0.038

0.575 ± 0.011

5.889 ± 0.041

7.478 ± 0.049

5.234 ± 0.037

7.108 ± 0.041

1.78 ± 0.024

7.465 ± 0.04

5.524 ± 0.047

9.396 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.02

5.071 ± 0.036

3.772 ± 0.024

3.415 ± 0.024

4.205 ± 0.029

7.339 ± 0.043

5.69 ± 0.049

6.431 ± 0.032

1.223 ± 0.017

3.774 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski