Amino acid dipepetide frequency for Salvia splendens (Scarlet sage)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.913AlaAla: 7.913 ± 0.03	1.275AlaCys: 1.275 ± 0.009	3.528AlaAsp: 3.528 ± 0.011	4.694AlaGlu: 4.694 ± 0.016	2.808AlaPhe: 2.808 ± 0.012	4.467AlaGly: 4.467 ± 0.016	1.371AlaHis: 1.371 ± 0.009	3.812AlaIle: 3.812 ± 0.015	4.004AlaLys: 4.004 ± 0.016	6.909AlaLeu: 6.909 ± 0.023	1.935AlaMet: 1.935 ± 0.01	2.663AlaAsn: 2.663 ± 0.014	3.147AlaPro: 3.147 ± 0.016	2.188AlaGln: 2.188 ± 0.011	3.769AlaArg: 3.769 ± 0.015	6.347AlaSer: 6.347 ± 0.021	3.77AlaThr: 3.77 ± 0.013	5.349AlaVal: 5.349 ± 0.021	0.794AlaTrp: 0.794 ± 0.006	1.892AlaTyr: 1.892 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.073CysAla: 1.073 ± 0.007	0.52CysCys: 0.52 ± 0.006	0.935CysAsp: 0.935 ± 0.007	0.895CysGlu: 0.895 ± 0.006	0.869CysPhe: 0.869 ± 0.008	1.423CysGly: 1.423 ± 0.009	0.452CysHis: 0.452 ± 0.005	1.02CysIle: 1.02 ± 0.008	1.083CysLys: 1.083 ± 0.007	1.881CysLeu: 1.881 ± 0.01	0.44CysMet: 0.44 ± 0.006	0.801CysAsn: 0.801 ± 0.006	0.895CysPro: 0.895 ± 0.007	0.567CysGln: 0.567 ± 0.005	1.125CysArg: 1.125 ± 0.007	1.811CysSer: 1.811 ± 0.01	0.856CysThr: 0.856 ± 0.006	1.145CysVal: 1.145 ± 0.008	0.246CysTrp: 0.246 ± 0.003	0.553CysTyr: 0.553 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.869AspAla: 3.869 ± 0.015	0.968AspCys: 0.968 ± 0.006	3.82AspAsp: 3.82 ± 0.016	4.281AspGlu: 4.281 ± 0.018	2.388AspPhe: 2.388 ± 0.01	3.941AspGly: 3.941 ± 0.013	1.261AspHis: 1.261 ± 0.007	2.955AspIle: 2.955 ± 0.015	2.653AspLys: 2.653 ± 0.013	5.209AspLeu: 5.209 ± 0.019	1.366AspMet: 1.366 ± 0.008	1.997AspAsn: 1.997 ± 0.01	2.632AspPro: 2.632 ± 0.011	1.728AspGln: 1.728 ± 0.009	2.562AspArg: 2.562 ± 0.012	4.377AspSer: 4.377 ± 0.015	2.233AspThr: 2.233 ± 0.011	3.815AspVal: 3.815 ± 0.015	0.713AspTrp: 0.713 ± 0.005	1.597AspTyr: 1.597 ± 0.009	0.0AspXaa: 0.0 ± 0.0
Glu	5.18GluAla: 5.18 ± 0.019	0.959GluCys: 0.959 ± 0.009	3.932GluAsp: 3.932 ± 0.017	6.157GluGlu: 6.157 ± 0.031	2.417GluPhe: 2.417 ± 0.011	3.784GluGly: 3.784 ± 0.013	1.275GluHis: 1.275 ± 0.008	3.829GluIle: 3.829 ± 0.017	4.696GluLys: 4.696 ± 0.022	6.023GluLeu: 6.023 ± 0.021	1.929GluMet: 1.929 ± 0.01	2.981GluAsn: 2.981 ± 0.014	2.088GluPro: 2.088 ± 0.011	2.111GluGln: 2.111 ± 0.011	3.551GluArg: 3.551 ± 0.014	4.748GluSer: 4.748 ± 0.018	3.06GluThr: 3.06 ± 0.014	4.238GluVal: 4.238 ± 0.015	0.741GluTrp: 0.741 ± 0.006	1.644GluTyr: 1.644 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.569PheAla: 2.569 ± 0.012	0.885PheCys: 0.885 ± 0.006	2.407PheAsp: 2.407 ± 0.009	2.297PheGlu: 2.297 ± 0.01	1.843PhePhe: 1.843 ± 0.01	3.011PheGly: 3.011 ± 0.012	1.08PheHis: 1.08 ± 0.007	1.922PheIle: 1.922 ± 0.01	1.983PheLys: 1.983 ± 0.011	4.222PheLeu: 4.222 ± 0.017	0.969PheMet: 0.969 ± 0.007	1.695PheAsn: 1.695 ± 0.009	2.09PhePro: 2.09 ± 0.01	1.452PheGln: 1.452 ± 0.008	2.068PheArg: 2.068 ± 0.01	3.974PheSer: 3.974 ± 0.014	1.971PheThr: 1.971 ± 0.01	2.795PheVal: 2.795 ± 0.012	0.554PheTrp: 0.554 ± 0.005	1.225PheTyr: 1.225 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	4.336GlyAla: 4.336 ± 0.018	1.314GlyCys: 1.314 ± 0.009	3.646GlyAsp: 3.646 ± 0.014	3.898GlyGlu: 3.898 ± 0.015	3.036GlyPhe: 3.036 ± 0.011	5.749GlyGly: 5.749 ± 0.027	1.502GlyHis: 1.502 ± 0.01	3.491GlyIle: 3.491 ± 0.014	4.046GlyLys: 4.046 ± 0.014	5.865GlyLeu: 5.865 ± 0.018	1.619GlyMet: 1.619 ± 0.008	3.066GlyAsn: 3.066 ± 0.016	2.355GlyPro: 2.355 ± 0.012	1.995GlyGln: 1.995 ± 0.01	3.836GlyArg: 3.836 ± 0.015	5.829GlySer: 5.829 ± 0.02	3.05GlyThr: 3.05 ± 0.013	4.505GlyVal: 4.505 ± 0.017	0.91GlyTrp: 0.91 ± 0.007	2.078GlyTyr: 2.078 ± 0.011	0.0GlyXaa: 0.0 ± 0.0
His	1.487HisAla: 1.487 ± 0.009	0.516HisCys: 0.516 ± 0.005	1.194HisAsp: 1.194 ± 0.007	1.294HisGlu: 1.294 ± 0.007	1.061HisPhe: 1.061 ± 0.008	1.706HisGly: 1.706 ± 0.009	0.939HisHis: 0.939 ± 0.008	1.208HisIle: 1.208 ± 0.007	1.168HisLys: 1.168 ± 0.007	2.454HisLeu: 2.454 ± 0.011	0.561HisMet: 0.561 ± 0.005	0.979HisAsn: 0.979 ± 0.007	1.394HisPro: 1.394 ± 0.009	0.982HisGln: 0.982 ± 0.006	1.469HisArg: 1.469 ± 0.01	1.963HisSer: 1.963 ± 0.01	1.009HisThr: 1.009 ± 0.007	1.546HisVal: 1.546 ± 0.009	0.29HisTrp: 0.29 ± 0.004	0.691HisTyr: 0.691 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.654IleAla: 3.654 ± 0.016	1.114IleCys: 1.114 ± 0.008	2.893IleAsp: 2.893 ± 0.013	3.099IleGlu: 3.099 ± 0.013	2.209IlePhe: 2.209 ± 0.01	3.38IleGly: 3.38 ± 0.012	1.323IleHis: 1.323 ± 0.008	2.58IleIle: 2.58 ± 0.011	2.786IleLys: 2.786 ± 0.011	5.185IleLeu: 5.185 ± 0.016	1.117IleMet: 1.117 ± 0.007	2.08IleAsn: 2.08 ± 0.01	2.905IlePro: 2.905 ± 0.015	1.871IleGln: 1.871 ± 0.009	2.682IleArg: 2.682 ± 0.012	4.773IleSer: 4.773 ± 0.016	2.487IleThr: 2.487 ± 0.011	3.434IleVal: 3.434 ± 0.012	0.671IleTrp: 0.671 ± 0.006	1.483IleTyr: 1.483 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	4.066LysAla: 4.066 ± 0.015	0.97LysCys: 0.97 ± 0.007	3.098LysAsp: 3.098 ± 0.015	4.319LysGlu: 4.319 ± 0.023	2.112LysPhe: 2.112 ± 0.01	3.464LysGly: 3.464 ± 0.013	1.353LysHis: 1.353 ± 0.009	3.188LysIle: 3.188 ± 0.013	4.536LysLys: 4.536 ± 0.022	5.904LysLeu: 5.904 ± 0.02	1.576LysMet: 1.576 ± 0.009	2.482LysAsn: 2.482 ± 0.012	2.654LysPro: 2.654 ± 0.013	2.213LysGln: 2.213 ± 0.01	3.544LysArg: 3.544 ± 0.013	4.629LysSer: 4.629 ± 0.019	2.734LysThr: 2.734 ± 0.011	3.672LysVal: 3.672 ± 0.014	0.773LysTrp: 0.773 ± 0.006	1.568LysTyr: 1.568 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	6.719LeuAla: 6.719 ± 0.02	1.857LeuCys: 1.857 ± 0.011	5.234LeuAsp: 5.234 ± 0.017	6.262LeuGlu: 6.262 ± 0.024	3.772LeuPhe: 3.772 ± 0.015	5.75LeuGly: 5.75 ± 0.018	2.669LeuHis: 2.669 ± 0.011	4.626LeuIle: 4.626 ± 0.015	5.964LeuLys: 5.964 ± 0.022	9.997LeuLeu: 9.997 ± 0.03	2.238LeuMet: 2.238 ± 0.011	3.893LeuAsn: 3.893 ± 0.013	5.104LeuPro: 5.104 ± 0.02	4.187LeuGln: 4.187 ± 0.015	5.56LeuArg: 5.56 ± 0.018	8.617LeuSer: 8.617 ± 0.029	4.413LeuThr: 4.413 ± 0.016	6.395LeuVal: 6.395 ± 0.018	1.162LeuTrp: 1.162 ± 0.007	2.5LeuTyr: 2.5 ± 0.012	0.0LeuXaa: 0.0 ± 0.0
Met	2.22MetAla: 2.22 ± 0.011	0.355MetCys: 0.355 ± 0.004	1.457MetAsp: 1.457 ± 0.009	2.009MetGlu: 2.009 ± 0.011	0.841MetPhe: 0.841 ± 0.006	1.555MetGly: 1.555 ± 0.008	0.567MetHis: 0.567 ± 0.005	1.227MetIle: 1.227 ± 0.008	1.653MetLys: 1.653 ± 0.009	2.341MetLeu: 2.341 ± 0.01	0.732MetMet: 0.732 ± 0.005	1.026MetAsn: 1.026 ± 0.008	1.106MetPro: 1.106 ± 0.007	0.98MetGln: 0.98 ± 0.007	1.327MetArg: 1.327 ± 0.008	1.856MetSer: 1.856 ± 0.008	1.036MetThr: 1.036 ± 0.007	1.677MetVal: 1.677 ± 0.01	0.296MetTrp: 0.296 ± 0.003	0.635MetTyr: 0.635 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.819AsnAla: 2.819 ± 0.011	0.82AsnCys: 0.82 ± 0.006	2.025AsnAsp: 2.025 ± 0.01	2.494AsnGlu: 2.494 ± 0.012	1.829AsnPhe: 1.829 ± 0.01	3.224AsnGly: 3.224 ± 0.014	1.085AsnHis: 1.085 ± 0.007	2.295AsnIle: 2.295 ± 0.013	2.215AsnLys: 2.215 ± 0.01	4.409AsnLeu: 4.409 ± 0.019	1.112AsnMet: 1.112 ± 0.007	1.999AsnAsn: 1.999 ± 0.012	2.307AsnPro: 2.307 ± 0.011	1.615AsnGln: 1.615 ± 0.01	2.009AsnArg: 2.009 ± 0.009	3.773AsnSer: 3.773 ± 0.016	1.88AsnThr: 1.88 ± 0.01	2.769AsnVal: 2.769 ± 0.011	0.543AsnTrp: 0.543 ± 0.005	1.264AsnTyr: 1.264 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	3.328ProAla: 3.328 ± 0.014	0.757ProCys: 0.757 ± 0.005	2.572ProAsp: 2.572 ± 0.01	3.148ProGlu: 3.148 ± 0.013	1.947ProPhe: 1.947 ± 0.009	2.701ProGly: 2.701 ± 0.013	1.188ProHis: 1.188 ± 0.007	2.266ProIle: 2.266 ± 0.011	2.598ProLys: 2.598 ± 0.013	4.333ProLeu: 4.333 ± 0.014	0.96ProMet: 0.96 ± 0.007	2.266ProAsn: 2.266 ± 0.011	4.085ProPro: 4.085 ± 0.029	1.849ProGln: 1.849 ± 0.012	2.514ProArg: 2.514 ± 0.01	5.041ProSer: 5.041 ± 0.017	2.581ProThr: 2.581 ± 0.011	3.085ProVal: 3.085 ± 0.013	0.575ProTrp: 0.575 ± 0.006	1.299ProTyr: 1.299 ± 0.009	0.0ProXaa: 0.0 ± 0.0
Gln	2.331GlnAla: 2.331 ± 0.012	0.554GlnCys: 0.554 ± 0.006	1.601GlnAsp: 1.601 ± 0.008	2.305GlnGlu: 2.305 ± 0.011	1.36GlnPhe: 1.36 ± 0.008	2.045GlnGly: 2.045 ± 0.011	0.927GlnHis: 0.927 ± 0.007	1.972GlnIle: 1.972 ± 0.009	2.176GlnLys: 2.176 ± 0.012	3.587GlnLeu: 3.587 ± 0.015	0.954GlnMet: 0.954 ± 0.007	1.675GlnAsn: 1.675 ± 0.009	1.781GlnPro: 1.781 ± 0.011	1.921GlnGln: 1.921 ± 0.018	2.103GlnArg: 2.103 ± 0.01	2.813GlnSer: 2.813 ± 0.012	1.662GlnThr: 1.662 ± 0.009	2.254GlnVal: 2.254 ± 0.011	0.451GlnTrp: 0.451 ± 0.004	0.902GlnTyr: 0.902 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.631ArgAla: 3.631 ± 0.015	1.027ArgCys: 1.027 ± 0.007	2.848ArgAsp: 2.848 ± 0.013	3.514ArgGlu: 3.514 ± 0.013	2.232ArgPhe: 2.232 ± 0.01	3.513ArgGly: 3.513 ± 0.015	1.358ArgHis: 1.358 ± 0.008	2.93ArgIle: 2.93 ± 0.011	3.758ArgLys: 3.758 ± 0.015	5.289ArgLeu: 5.289 ± 0.016	1.391ArgMet: 1.391 ± 0.008	2.49ArgAsn: 2.49 ± 0.01	2.451ArgPro: 2.451 ± 0.013	1.841ArgGln: 1.841 ± 0.009	4.373ArgArg: 4.373 ± 0.019	4.545ArgSer: 4.545 ± 0.017	2.339ArgThr: 2.339 ± 0.01	3.458ArgVal: 3.458 ± 0.013	0.762ArgTrp: 0.762 ± 0.006	1.481ArgTyr: 1.481 ± 0.009	0.0ArgXaa: 0.0 ± 0.0
Ser	5.899SerAla: 5.899 ± 0.017	1.741SerCys: 1.741 ± 0.01	4.683SerAsp: 4.683 ± 0.014	4.803SerGlu: 4.803 ± 0.021	3.809SerPhe: 3.809 ± 0.016	6.041SerGly: 6.041 ± 0.021	2.037SerHis: 2.037 ± 0.011	4.414SerIle: 4.414 ± 0.016	4.72SerLys: 4.72 ± 0.016	8.616SerLeu: 8.616 ± 0.024	2.084SerMet: 2.084 ± 0.01	3.892SerAsn: 3.892 ± 0.014	4.637SerPro: 4.637 ± 0.023	2.893SerGln: 2.893 ± 0.015	4.684SerArg: 4.684 ± 0.017	10.473SerSer: 10.473 ± 0.037	4.519SerThr: 4.519 ± 0.017	5.339SerVal: 5.339 ± 0.018	1.173SerTrp: 1.173 ± 0.008	2.324SerTyr: 2.324 ± 0.011	0.0SerXaa: 0.0 ± 0.0
Thr	3.603ThrAla: 3.603 ± 0.014	0.89ThrCys: 0.89 ± 0.007	2.296ThrAsp: 2.296 ± 0.012	2.717ThrGlu: 2.717 ± 0.013	1.964ThrPhe: 1.964 ± 0.01	3.216ThrGly: 3.216 ± 0.014	0.994ThrHis: 0.994 ± 0.007	2.571ThrIle: 2.571 ± 0.011	2.521ThrLys: 2.521 ± 0.012	4.557ThrLeu: 4.557 ± 0.014	1.131ThrMet: 1.131 ± 0.007	1.988ThrAsn: 1.988 ± 0.01	2.628ThrPro: 2.628 ± 0.014	1.416ThrGln: 1.416 ± 0.008	2.404ThrArg: 2.404 ± 0.01	4.443ThrSer: 4.443 ± 0.016	2.8ThrThr: 2.8 ± 0.013	3.273ThrVal: 3.273 ± 0.013	0.618ThrTrp: 0.618 ± 0.005	1.393ThrTyr: 1.393 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	5.248ValAla: 5.248 ± 0.021	1.205ValCys: 1.205 ± 0.008	3.916ValAsp: 3.916 ± 0.015	4.766ValGlu: 4.766 ± 0.016	2.735ValPhe: 2.735 ± 0.013	4.323ValGly: 4.323 ± 0.013	1.519ValHis: 1.519 ± 0.009	3.295ValIle: 3.295 ± 0.015	3.893ValLys: 3.893 ± 0.014	6.188ValLeu: 6.188 ± 0.018	1.643ValMet: 1.643 ± 0.009	2.55ValAsn: 2.55 ± 0.011	3.106ValPro: 3.106 ± 0.014	2.226ValGln: 2.226 ± 0.01	3.326ValArg: 3.326 ± 0.013	5.508ValSer: 5.508 ± 0.016	3.08ValThr: 3.08 ± 0.012	5.403ValVal: 5.403 ± 0.02	0.805ValTrp: 0.805 ± 0.007	1.99ValTyr: 1.99 ± 0.01	0.0ValXaa: 0.0 ± 0.0
Trp	0.787TrpAla: 0.787 ± 0.007	0.246TrpCys: 0.246 ± 0.003	0.712TrpAsp: 0.712 ± 0.006	0.775TrpGlu: 0.775 ± 0.006	0.521TrpPhe: 0.521 ± 0.005	0.761TrpGly: 0.761 ± 0.006	0.298TrpHis: 0.298 ± 0.004	0.695TrpIle: 0.695 ± 0.006	0.933TrpLys: 0.933 ± 0.006	1.226TrpLeu: 1.226 ± 0.008	0.359TrpMet: 0.359 ± 0.004	0.666TrpAsn: 0.666 ± 0.006	0.528TrpPro: 0.528 ± 0.005	0.437TrpGln: 0.437 ± 0.005	0.872TrpArg: 0.872 ± 0.006	0.986TrpSer: 0.986 ± 0.006	0.606TrpThr: 0.606 ± 0.005	0.723TrpVal: 0.723 ± 0.006	0.243TrpTrp: 0.243 ± 0.004	0.328TrpTyr: 0.328 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.905TyrAla: 1.905 ± 0.01	0.617TyrCys: 0.617 ± 0.006	1.566TyrAsp: 1.566 ± 0.009	1.617TyrGlu: 1.617 ± 0.008	1.258TyrPhe: 1.258 ± 0.008	2.074TyrGly: 2.074 ± 0.01	0.712TyrHis: 0.712 ± 0.006	1.431TyrIle: 1.431 ± 0.008	1.47TyrLys: 1.47 ± 0.009	2.722TyrLeu: 2.722 ± 0.012	0.779TyrMet: 0.779 ± 0.006	1.294TyrAsn: 1.294 ± 0.008	1.202TyrPro: 1.202 ± 0.007	0.904TyrGln: 0.904 ± 0.006	1.451TyrArg: 1.451 ± 0.007	2.274TyrSer: 2.274 ± 0.011	1.337TyrThr: 1.337 ± 0.008	1.85TyrVal: 1.85 ± 0.009	0.384TyrTrp: 0.384 ± 0.005	0.96TyrTyr: 0.96 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 52672 proteins (22732906 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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