Proteome-pI 2.0 - Proteome Isoelectric Point Database

Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group; Bacillus cereus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 5240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q81BB6|Q81BB6_BACCR Transcriptional regulator  AraC family OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=BC_3253 PE=4 SV=1

MM1 pKa = 7.43EE2 pKa = 4.09VHH4 pKa = 6.29GMVSIWFGNMQTQDD18 pKa = 3.46QLDD21 pKa = 3.91TYY23 pKa = 11.21IDD25 pKa = 3.39VTYY28 pKa = 10.72DD29 pKa = 3.41EE30 pKa = 5.52EE31 pKa = 6.58GDD33 pKa = 4.08SIPARR38 pKa = 11.84FFVDD42 pKa = 4.44FNIDD46 pKa = 3.74MIDD49 pKa = 3.3VDD51 pKa = 3.9EE52 pKa = 5.21DD53 pKa = 4.34FIEE56 pKa = 5.23KK57 pKa = 10.18EE58 pKa = 4.23VLEE61 pKa = 4.46EE62 pKa = 4.17ASDD65 pKa = 4.72DD66 pKa = 3.52ISMLLTGCSYY76 pKa = 11.47DD77 pKa = 4.37DD78 pKa = 5.35KK79 pKa = 10.84ILSRR83 pKa = 11.84IKK85 pKa = 10.62EE86 pKa = 4.09VVKK89 pKa = 10.83LNKK92 pKa = 9.95SYY94 pKa = 11.54NSIVLIYY101 pKa = 10.4NFQYY105 pKa = 11.23DD106 pKa = 3.78NSVSNCEE113 pKa = 3.67GFNFVTATSYY123 pKa = 11.29LL124 pKa = 3.7

Molecular weight: 14.32 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|Q814I5|Q814I5_BACCR Acetamide transporter OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=BC_5447 PE=3 SV=1

MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
5240	0	5240	1453967	18	5017	277.5	31.25

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.638 ± 0.042	0.831 ± 0.012	4.682 ± 0.028	7.555 ± 0.053	4.649 ± 0.028	6.728 ± 0.048	2.092 ± 0.021	8.07 ± 0.039	7.446 ± 0.052	9.463 ± 0.04

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.82 ± 0.02	4.7 ± 0.038	3.43 ± 0.029	3.728 ± 0.029	3.697 ± 0.028	5.83 ± 0.03	5.639 ± 0.052	7.238 ± 0.037	1.019 ± 0.013	3.744 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski