alpha proteobacterium BAL199

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8TNX4|A8TNX4_9PROT DUF126 domain-containing protein OS=alpha proteobacterium BAL199 OX=331869 GN=BAL199_12591 PE=4 SV=1
MM1 pKa = 7.27TVLGTDD7 pKa = 4.01DD8 pKa = 3.77RR9 pKa = 11.84VQVADD14 pKa = 4.01TTAFPYY20 pKa = 10.42SAIVQVRR27 pKa = 11.84VDD29 pKa = 3.94FDD31 pKa = 4.47GDD33 pKa = 3.3GSYY36 pKa = 11.15DD37 pKa = 3.26GWGTGAMISPNDD49 pKa = 3.66VLTAGHH55 pKa = 6.05VLWDD59 pKa = 3.65SQYY62 pKa = 11.47GYY64 pKa = 11.08AKK66 pKa = 10.2NIQVIPARR74 pKa = 11.84DD75 pKa = 3.58GGSTPFGTAEE85 pKa = 3.78GTAWYY90 pKa = 10.4VPDD93 pKa = 5.05AYY95 pKa = 9.78ISTGGTFAYY104 pKa = 10.41DD105 pKa = 2.69IGVINLGTSIGSSTGTLALQATSGPDD131 pKa = 3.33LVGTTVTTAGYY142 pKa = 9.53PGDD145 pKa = 3.87LSSNGSVMITTSGEE159 pKa = 3.66IDD161 pKa = 3.67GSIGDD166 pKa = 3.44NRR168 pKa = 11.84VYY170 pKa = 11.45YY171 pKa = 10.8NGTLDD176 pKa = 3.67SYY178 pKa = 11.26GGQSGSPLWVTVNGQTTIVGVHH200 pKa = 4.84TAGGSFYY207 pKa = 11.02NAGTALTSDD216 pKa = 5.07FYY218 pKa = 11.91SLIDD222 pKa = 3.34GWTGGDD228 pKa = 3.05INGSVVVVTSSTSGTASADD247 pKa = 3.68VITGSTGSDD256 pKa = 3.11TVSGYY261 pKa = 11.23AGDD264 pKa = 3.72DD265 pKa = 4.19TIWGEE270 pKa = 4.05SGNDD274 pKa = 3.24VIYY277 pKa = 10.69GNQGADD283 pKa = 4.01LISGQDD289 pKa = 3.5GNDD292 pKa = 3.75TIFGGQNQGPAGSDD306 pKa = 3.56GIARR310 pKa = 11.84NGTDD314 pKa = 3.96TIQGGDD320 pKa = 3.72GADD323 pKa = 3.14VLYY326 pKa = 11.29GNMGGDD332 pKa = 4.16LVDD335 pKa = 4.74GDD337 pKa = 4.39SGDD340 pKa = 4.65DD341 pKa = 3.33ILYY344 pKa = 10.59GGQDD348 pKa = 3.18SDD350 pKa = 3.95TLRR353 pKa = 11.84GGDD356 pKa = 3.3GADD359 pKa = 2.46RR360 pKa = 11.84VFGNLHH366 pKa = 6.79NDD368 pKa = 3.45TLSGGGGNDD377 pKa = 3.29TLLGGDD383 pKa = 4.29GNDD386 pKa = 3.92LLSGGSGDD394 pKa = 4.58DD395 pKa = 4.53LLYY398 pKa = 11.08GDD400 pKa = 4.83NLFSGVVTLTRR411 pKa = 11.84RR412 pKa = 11.84VSKK415 pKa = 9.71LACRR419 pKa = 11.84SLIKK423 pKa = 10.38RR424 pKa = 11.84HH425 pKa = 5.24VPRR428 pKa = 11.84ISKK431 pKa = 10.06LRR433 pKa = 11.84TLRR436 pKa = 11.84ADD438 pKa = 3.27GSEE441 pKa = 3.84WW442 pKa = 3.2

Molecular weight: 44.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8TUE9|A8TUE9_9PROT Probable membrane transporter protein OS=alpha proteobacterium BAL199 OX=331869 GN=BAL199_15107 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.1QPSVLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VIATRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5881

0

5881

1761557

13

8056

299.5

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.603 ± 0.047

0.877 ± 0.01

6.199 ± 0.028

5.403 ± 0.026

3.469 ± 0.02

8.741 ± 0.036

2.117 ± 0.014

4.862 ± 0.021

2.693 ± 0.027

10.091 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.016

2.299 ± 0.017

5.375 ± 0.025

2.815 ± 0.018

7.767 ± 0.035

5.159 ± 0.023

5.506 ± 0.019

8.019 ± 0.029

1.43 ± 0.014

2.149 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski