Bacillus sp. SG-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6CJV0|A6CJV0_9BACI Uncharacterized protein OS=Bacillus sp. SG-1 OX=161544 GN=BSG1_16545 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.33GWDD5 pKa = 3.58TMNLEE10 pKa = 3.8WFDD13 pKa = 3.82RR14 pKa = 11.84VSGEE18 pKa = 4.03LQEE21 pKa = 4.46HH22 pKa = 6.64LEE24 pKa = 4.52SICEE28 pKa = 3.94KK29 pKa = 10.09YY30 pKa = 10.85DD31 pKa = 3.37RR32 pKa = 11.84VGQMNVDD39 pKa = 3.95RR40 pKa = 11.84GAKK43 pKa = 9.2HH44 pKa = 6.15PRR46 pKa = 11.84LEE48 pKa = 4.59FYY50 pKa = 11.19VEE52 pKa = 4.2TEE54 pKa = 3.75DD55 pKa = 4.03QEE57 pKa = 5.45RR58 pKa = 11.84DD59 pKa = 3.78FFCTLFYY66 pKa = 11.12DD67 pKa = 4.59PYY69 pKa = 10.96NDD71 pKa = 3.29EE72 pKa = 5.68FYY74 pKa = 11.22LEE76 pKa = 5.06SYY78 pKa = 10.52DD79 pKa = 3.47IDD81 pKa = 3.54YY82 pKa = 10.8GQTLKK87 pKa = 11.2VILPDD92 pKa = 3.36IEE94 pKa = 5.56DD95 pKa = 4.11IIDD98 pKa = 3.85AVHH101 pKa = 6.73EE102 pKa = 4.34SLHH105 pKa = 7.23DD106 pKa = 3.89YY107 pKa = 11.39LSMDD111 pKa = 4.21EE112 pKa = 4.1IDD114 pKa = 5.82FYY116 pKa = 11.9DD117 pKa = 4.18EE118 pKa = 4.13ADD120 pKa = 4.23EE121 pKa = 5.06YY122 pKa = 11.64DD123 pKa = 4.96DD124 pKa = 5.54DD125 pKa = 6.79HH126 pKa = 8.81IDD128 pKa = 4.09YY129 pKa = 10.79IVDD132 pKa = 4.7DD133 pKa = 4.6EE134 pKa = 6.19DD135 pKa = 4.04EE136 pKa = 5.26DD137 pKa = 5.47GPVHH141 pKa = 6.29YY142 pKa = 10.94VVDD145 pKa = 4.91DD146 pKa = 3.58YY147 pKa = 11.83DD148 pKa = 4.22YY149 pKa = 11.07DD150 pKa = 4.49YY151 pKa = 11.52EE152 pKa = 4.37DD153 pKa = 4.49QADD156 pKa = 3.81EE157 pKa = 5.57DD158 pKa = 3.77ILEE161 pKa = 4.61EE162 pKa = 5.38IEE164 pKa = 4.69VEE166 pKa = 4.06WEE168 pKa = 4.27TPEE171 pKa = 3.81VTAFIHH177 pKa = 6.0GDD179 pKa = 3.61EE180 pKa = 4.76VEE182 pKa = 4.08VTYY185 pKa = 10.81QFGIITEE192 pKa = 4.32TGDD195 pKa = 3.71GVLKK199 pKa = 10.34RR200 pKa = 11.84LNRR203 pKa = 11.84IITEE207 pKa = 4.1DD208 pKa = 3.4NEE210 pKa = 4.58LIKK213 pKa = 11.06DD214 pKa = 3.53EE215 pKa = 4.41SHH217 pKa = 7.38FIFSKK222 pKa = 10.96EE223 pKa = 3.74EE224 pKa = 3.86ANTIIAMIASHH235 pKa = 6.34SDD237 pKa = 2.99KK238 pKa = 10.83LSEE241 pKa = 4.4FSFHH245 pKa = 6.96

Molecular weight: 29.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6CPP1|A6CPP1_9BACI Nucleoid occlusion protein OS=Bacillus sp. SG-1 OX=161544 GN=noc PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.55RR3 pKa = 11.84TFQPNSRR10 pKa = 11.84KK11 pKa = 9.5RR12 pKa = 11.84SKK14 pKa = 10.67NHH16 pKa = 4.97GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 8.81VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4305

0

4305

1053293

20

1495

244.7

27.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.846 ± 0.042

0.677 ± 0.011

5.163 ± 0.032

8.065 ± 0.048

4.687 ± 0.035

7.057 ± 0.036

2.033 ± 0.019

7.528 ± 0.039

6.969 ± 0.038

9.809 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.021

4.32 ± 0.024

3.575 ± 0.025

3.52 ± 0.025

4.079 ± 0.028

6.264 ± 0.029

5.189 ± 0.028

6.807 ± 0.032

1.05 ± 0.015

3.483 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski