Propionimicrobium lymphophilum ACS-093-V-SCH5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Propionimicrobium; Propionimicrobium lymphophilum

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S2VYT4|S2VYT4_9ACTN Uncharacterized protein OS=Propionimicrobium lymphophilum ACS-093-V-SCH5 OX=883161 GN=HMPREF9306_01377 PE=4 SV=1
MM1 pKa = 6.39ITNYY5 pKa = 10.07KK6 pKa = 7.62ITGMTCGHH14 pKa = 6.76CVNAVTSEE22 pKa = 3.81VSEE25 pKa = 4.1IDD27 pKa = 3.26GVTDD31 pKa = 3.4VDD33 pKa = 3.93VQLEE37 pKa = 4.27GGKK40 pKa = 8.58MAVTSEE46 pKa = 4.2SEE48 pKa = 3.72IDD50 pKa = 3.56FEE52 pKa = 5.09AIKK55 pKa = 10.42EE56 pKa = 4.27AVDD59 pKa = 3.27EE60 pKa = 4.83AGDD63 pKa = 3.89YY64 pKa = 9.83TVEE67 pKa = 4.07LAA69 pKa = 4.92

Molecular weight:
7.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S2W4D3|S2W4D3_9ACTN DNA gyrase subunit B OS=Propionimicrobium lymphophilum ACS-093-V-SCH5 OX=883161 GN=gyrB PE=3 SV=1
MM1 pKa = 7.05NALVAGLGAFLFLFGAFVVVVGARR25 pKa = 11.84SSSSRR30 pKa = 11.84IVSKK34 pKa = 10.33PLNLPKK40 pKa = 10.26ISVRR44 pKa = 11.84TGGVLALSALFALVSGWLPALIVGPGVGLVLPDD77 pKa = 4.06LLRR80 pKa = 11.84PKK82 pKa = 10.23PNRR85 pKa = 11.84EE86 pKa = 4.02VQLLAALDD94 pKa = 3.37RR95 pKa = 11.84WVRR98 pKa = 11.84LLTASVATGKK108 pKa = 10.46SIPDD112 pKa = 3.45AMRR115 pKa = 11.84TTYY118 pKa = 10.32AQCPKK123 pKa = 8.93MLKK126 pKa = 10.16SGVSTALARR135 pKa = 11.84LRR137 pKa = 11.84GRR139 pKa = 11.84WSVRR143 pKa = 11.84EE144 pKa = 3.97SLLALADD151 pKa = 3.68EE152 pKa = 4.79WDD154 pKa = 3.86STDD157 pKa = 3.59ADD159 pKa = 3.79AVLAACILCSEE170 pKa = 4.3RR171 pKa = 11.84GGLGAHH177 pKa = 6.14RR178 pKa = 11.84TLDD181 pKa = 3.56ALADD185 pKa = 3.99SVSDD189 pKa = 3.29RR190 pKa = 11.84LAALRR195 pKa = 11.84EE196 pKa = 3.87IDD198 pKa = 4.07AEE200 pKa = 4.12RR201 pKa = 11.84AKK203 pKa = 10.66PRR205 pKa = 11.84VVVRR209 pKa = 11.84QVTMITVVVLGAGVVINPSYY229 pKa = 10.27FAPFTTPIGQVLAIGFFVCYY249 pKa = 9.29MLSLRR254 pKa = 11.84MLQRR258 pKa = 11.84MSRR261 pKa = 11.84PQKK264 pKa = 10.14RR265 pKa = 11.84EE266 pKa = 3.48RR267 pKa = 11.84ILQVNQQ273 pKa = 3.01

Molecular weight:
29.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2076

0

2076

682374

31

2653

328.7

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.571 ± 0.067

0.942 ± 0.015

6.086 ± 0.041

6.39 ± 0.045

3.397 ± 0.037

8.034 ± 0.049

1.773 ± 0.022

5.437 ± 0.042

4.699 ± 0.044

9.552 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.026

3.382 ± 0.029

4.735 ± 0.03

3.466 ± 0.03

5.726 ± 0.053

6.368 ± 0.042

5.455 ± 0.042

7.886 ± 0.05

1.417 ± 0.019

2.369 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski