Circovirus-like genome DHCV-6

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHH1|A0A190WHH1_9CIRC Uncharacterized protein OS=Circovirus-like genome DHCV-6 OX=1788455 PE=4 SV=1
MM1 pKa = 7.27SRR3 pKa = 11.84HH4 pKa = 5.85RR5 pKa = 11.84NYY7 pKa = 10.67CFTYY11 pKa = 9.97NNYY14 pKa = 9.96PNTEE18 pKa = 3.73LVDD21 pKa = 3.85NVNCKK26 pKa = 10.3YY27 pKa = 10.0IAYY30 pKa = 8.56AHH32 pKa = 6.21EE33 pKa = 4.23VAPTTGTRR41 pKa = 11.84HH42 pKa = 4.78LQGYY46 pKa = 8.95IAFHH50 pKa = 4.88STKK53 pKa = 9.54TLAAARR59 pKa = 11.84RR60 pKa = 11.84LLPGCHH66 pKa = 6.67LSAMAGSISQNDD78 pKa = 4.27AYY80 pKa = 10.66CSKK83 pKa = 10.74SGTLIEE89 pKa = 4.94RR90 pKa = 11.84GEE92 pKa = 4.27KK93 pKa = 9.45PASNDD98 pKa = 3.21DD99 pKa = 3.2KK100 pKa = 11.69GRR102 pKa = 11.84AEE104 pKa = 3.88KK105 pKa = 10.78LRR107 pKa = 11.84WQRR110 pKa = 11.84ARR112 pKa = 11.84DD113 pKa = 3.45LAKK116 pKa = 10.64AGNLDD121 pKa = 4.47EE122 pKa = 5.82IDD124 pKa = 3.18ADD126 pKa = 3.63IYY128 pKa = 10.04IRR130 pKa = 11.84CYY132 pKa = 9.74STLKK136 pKa = 10.68SISKK140 pKa = 10.24DD141 pKa = 3.21HH142 pKa = 6.55MIKK145 pKa = 10.55PEE147 pKa = 4.14PVDD150 pKa = 4.42VKK152 pKa = 10.49CFWIHH157 pKa = 6.23GATGTGKK164 pKa = 10.07SYY166 pKa = 10.77CVEE169 pKa = 3.89TTYY172 pKa = 11.04PDD174 pKa = 3.94CYY176 pKa = 9.91KK177 pKa = 11.1KK178 pKa = 11.3SMDD181 pKa = 3.7DD182 pKa = 3.91LKK184 pKa = 11.14WFDD187 pKa = 4.55GYY189 pKa = 11.55DD190 pKa = 3.7NQDD193 pKa = 2.79VVYY196 pKa = 10.96LEE198 pKa = 5.4DD199 pKa = 4.05FDD201 pKa = 5.32VYY203 pKa = 9.93QIKK206 pKa = 9.79WGGLMKK212 pKa = 10.59RR213 pKa = 11.84LADD216 pKa = 4.28RR217 pKa = 11.84WPMQASIKK225 pKa = 10.8GSMKK229 pKa = 10.25YY230 pKa = 9.82IRR232 pKa = 11.84PKK234 pKa = 10.36IVIVTSNYY242 pKa = 8.38TPNEE246 pKa = 3.36IWMDD250 pKa = 3.74PQTVEE255 pKa = 3.56PLLRR259 pKa = 11.84RR260 pKa = 11.84FKK262 pKa = 10.95VIEE265 pKa = 4.26KK266 pKa = 9.97VSQEE270 pKa = 3.91QVVDD274 pKa = 4.49FTT276 pKa = 4.81

Molecular weight: 31.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHP0|A0A190WHP0_9CIRC Uncharacterized protein OS=Circovirus-like genome DHCV-6 OX=1788455 PE=4 SV=1
MM1 pKa = 7.67LIASISLMLMRR12 pKa = 11.84LLQQLAPDD20 pKa = 3.88ISRR23 pKa = 11.84VILLSIQQKK32 pKa = 10.2LLQLLGDD39 pKa = 4.43FYY41 pKa = 11.65RR42 pKa = 11.84DD43 pKa = 3.98AISQLWRR50 pKa = 11.84DD51 pKa = 3.96LYY53 pKa = 10.35PRR55 pKa = 11.84TMRR58 pKa = 11.84TARR61 pKa = 11.84KK62 pKa = 9.02VARR65 pKa = 11.84LLNGGRR71 pKa = 11.84NLLPMTIRR79 pKa = 11.84DD80 pKa = 3.63VPRR83 pKa = 11.84NSDD86 pKa = 3.14GSALEE91 pKa = 3.99ILLKK95 pKa = 10.8LVTWTKK101 pKa = 11.19LMLIFISVAILLLNQYY117 pKa = 10.73LKK119 pKa = 10.54III121 pKa = 4.2

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

619

121

276

206.3

23.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.947 ± 0.399

1.292 ± 0.83

5.493 ± 1.164

2.908 ± 0.95

3.231 ± 0.792

5.977 ± 1.061

1.454 ± 0.588

6.947 ± 1.107

7.108 ± 0.864

10.662 ± 3.689

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.231 ± 0.506

4.362 ± 0.35

3.393 ± 0.545

4.685 ± 0.736

5.654 ± 1.167

7.431 ± 0.899

5.816 ± 0.491

7.108 ± 1.494

1.292 ± 0.579

5.008 ± 0.787
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski