Puniceibacterium sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Puniceibacterium

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238XQN4|A0A238XQN4_9RHOB Nitrogen regulatory protein P-II OS=Puniceibacterium sediminis OX=1608407 GN=SAMN06265370_112117 PE=3 SV=1
MM1 pKa = 7.26ATSLDD6 pKa = 3.35WSAPCAGIQPEE17 pKa = 4.41VPKK20 pKa = 10.42TEE22 pKa = 3.87KK23 pKa = 10.17TNPVSNQKK31 pKa = 9.78VDD33 pKa = 3.8EE34 pKa = 4.19VLKK37 pKa = 10.29PVSNRR42 pKa = 11.84FLRR45 pKa = 11.84MLCSGTAAITVLSSPALAEE64 pKa = 4.26TCSVSPTFVNWITPGTLGGLNVDD87 pKa = 4.67QSATLQWGSAATDD100 pKa = 3.38ISISGPLFIFGGEE113 pKa = 4.01TSITRR118 pKa = 11.84ISEE121 pKa = 4.26YY122 pKa = 9.54PDD124 pKa = 3.11SRR126 pKa = 11.84GTYY129 pKa = 9.35SLNFGSPVVHH139 pKa = 7.35DD140 pKa = 4.31LNIFVGNVGRR150 pKa = 11.84NGFQPLEE157 pKa = 4.12SGIVGDD163 pKa = 5.39FNMTLSGGGTAEE175 pKa = 4.05NLFGAVTPLDD185 pKa = 3.6NQGYY189 pKa = 7.83FQIFGSDD196 pKa = 3.34TEE198 pKa = 5.67GIAQTALTGTGADD211 pKa = 4.22GLPLDD216 pKa = 4.32GKK218 pKa = 10.73FWVHH222 pKa = 7.08DD223 pKa = 4.28PNLVPGDD230 pKa = 3.87INNGGEE236 pKa = 3.92DD237 pKa = 3.24QAYY240 pKa = 10.59GMIDD244 pKa = 3.78LAYY247 pKa = 7.44TAPTVVSGGSDD258 pKa = 3.67TITQLTLRR266 pKa = 11.84QVGVGVGPTVTLGVQARR283 pKa = 11.84LRR285 pKa = 11.84SCINAADD292 pKa = 4.78DD293 pKa = 4.84DD294 pKa = 4.8FSSAPIASASGGSTGSVLGDD314 pKa = 3.63DD315 pKa = 4.69LLNGGGVDD323 pKa = 3.71TTPLLGNVDD332 pKa = 4.33LAVVSLPTPAVGAINLATDD351 pKa = 3.28TGEE354 pKa = 3.93ITVEE358 pKa = 3.87AGTTPGTYY366 pKa = 9.4FVEE369 pKa = 4.39YY370 pKa = 10.37SLTDD374 pKa = 3.27RR375 pKa = 11.84TTDD378 pKa = 3.43GTPTNTDD385 pKa = 2.82TATATVVVGGAAAGTIVAVNDD406 pKa = 4.86DD407 pKa = 4.04YY408 pKa = 11.96TSTPVNAAFGGSAGEE423 pKa = 4.14VLTNDD428 pKa = 3.68TANGIPALFPNVTLSVLTQAVPASPGDD455 pKa = 3.69PVPTLVTSGIDD466 pKa = 3.01AGRR469 pKa = 11.84VVVPSGVPAGVYY481 pKa = 9.72TIDD484 pKa = 4.22YY485 pKa = 8.69LLCDD489 pKa = 4.07AVNSADD495 pKa = 4.32CDD497 pKa = 3.82TATVTIAVFEE507 pKa = 4.48GNGLDD512 pKa = 5.18FGDD515 pKa = 4.76APLSYY520 pKa = 10.53LIPTHH525 pKa = 6.6AVASTPTIYY534 pKa = 10.65LGAVPPDD541 pKa = 3.82TEE543 pKa = 5.66LVAQSDD549 pKa = 4.15GTATGDD555 pKa = 5.39DD556 pKa = 3.4IADD559 pKa = 3.87TNDD562 pKa = 3.13EE563 pKa = 4.78DD564 pKa = 5.44GVTFPILTQGLSVTLDD580 pKa = 3.33VEE582 pKa = 4.72VTGNGYY588 pKa = 8.9LQAWLDD594 pKa = 3.68FDD596 pKa = 5.31GDD598 pKa = 4.27GLFATAPVEE607 pKa = 5.22RR608 pKa = 11.84IATDD612 pKa = 3.4LRR614 pKa = 11.84DD615 pKa = 5.06DD616 pKa = 3.97GTSDD620 pKa = 4.79DD621 pKa = 4.44NVAGDD626 pKa = 5.2GIIQINITVPDD637 pKa = 4.57DD638 pKa = 3.35ATTDD642 pKa = 3.28LTFARR647 pKa = 11.84FRR649 pKa = 11.84YY650 pKa = 9.81GSEE653 pKa = 3.72QGLGTTSFAVDD664 pKa = 4.17GEE666 pKa = 4.53VEE668 pKa = 4.55DD669 pKa = 4.24YY670 pKa = 11.55SLIIAAADD678 pKa = 3.48LVDD681 pKa = 5.34RR682 pKa = 11.84GDD684 pKa = 3.89APASYY689 pKa = 10.23GDD691 pKa = 3.71PRR693 pKa = 11.84HH694 pKa = 5.78VVVPQIYY701 pKa = 10.06LGSALPDD708 pKa = 3.68TEE710 pKa = 4.06TSTRR714 pKa = 11.84FSTDD718 pKa = 2.38ADD720 pKa = 3.9GDD722 pKa = 4.22DD723 pKa = 4.91LSGSDD728 pKa = 5.68DD729 pKa = 3.65EE730 pKa = 5.71DD731 pKa = 3.38ATTFPQLVAGTTVPLTVQTHH751 pKa = 4.89EE752 pKa = 4.25TLSVQFDD759 pKa = 3.71LGLPVLVPGITNLQLWIDD777 pKa = 4.09FDD779 pKa = 4.29QNGVFDD785 pKa = 3.82TSEE788 pKa = 3.92QVAVDD793 pKa = 3.75YY794 pKa = 11.12RR795 pKa = 11.84DD796 pKa = 3.54GGTGDD801 pKa = 3.35TDD803 pKa = 3.54GTFNNQISLNIPVPTDD819 pKa = 2.79IGNGTTYY826 pKa = 11.24ARR828 pKa = 11.84LRR830 pKa = 11.84WSTTSGIAADD840 pKa = 4.55PFDD843 pKa = 4.49GLNMDD848 pKa = 5.16GEE850 pKa = 4.61VEE852 pKa = 4.48DD853 pKa = 4.54YY854 pKa = 11.12LVTLSNPNGPLTCSSNFYY872 pKa = 10.02MVATEE877 pKa = 4.16PAQNLPALDD886 pKa = 3.98EE887 pKa = 4.33LSISEE892 pKa = 4.14SGGIYY897 pKa = 9.64TLSHH901 pKa = 6.55TDD903 pKa = 4.03LPPDD907 pKa = 3.26YY908 pKa = 9.75TGNYY912 pKa = 9.56LVTGWGYY919 pKa = 11.41NEE921 pKa = 4.56LDD923 pKa = 3.65GYY925 pKa = 10.61IYY927 pKa = 10.76GVGQSPRR934 pKa = 11.84NLYY937 pKa = 9.81QINASGAVRR946 pKa = 11.84AVADD950 pKa = 3.46ISGLSLEE957 pKa = 4.83SPDD960 pKa = 3.72TSSDD964 pKa = 3.06ILPNGIMIYY973 pKa = 9.97MSGTDD978 pKa = 3.45FSRR981 pKa = 11.84YY982 pKa = 7.88QLLDD986 pKa = 2.89ISDD989 pKa = 4.18PANPVALGVLTAEE1002 pKa = 4.53AGAAYY1007 pKa = 9.61GRR1009 pKa = 11.84DD1010 pKa = 3.19MAYY1013 pKa = 10.51NPRR1016 pKa = 11.84DD1017 pKa = 3.31GLIYY1021 pKa = 10.43FVDD1024 pKa = 4.09TSNNLHH1030 pKa = 6.52SFDD1033 pKa = 4.39PRR1035 pKa = 11.84NGVPGAITVDD1045 pKa = 4.0PVASLPLPAGKK1056 pKa = 10.2FSIDD1060 pKa = 3.26IDD1062 pKa = 4.26SVWFDD1067 pKa = 3.39GSGFMYY1073 pKa = 10.74AFDD1076 pKa = 3.7NQSRR1080 pKa = 11.84QVFALEE1086 pKa = 4.13VGSVGARR1093 pKa = 11.84PASYY1097 pKa = 10.71QFIEE1101 pKa = 4.25VLGTIDD1107 pKa = 3.44NLTYY1111 pKa = 10.4QGNDD1115 pKa = 3.41GASCRR1120 pKa = 11.84APGPFVSSIFAEE1132 pKa = 4.81GAISGTLYY1140 pKa = 10.71QDD1142 pKa = 3.81ADD1144 pKa = 3.73GSDD1147 pKa = 3.36TRR1149 pKa = 11.84DD1150 pKa = 3.16TGEE1153 pKa = 4.34PGLPAGITVTLHH1165 pKa = 7.35DD1166 pKa = 5.53DD1167 pKa = 3.54NGTPADD1173 pKa = 4.11PADD1176 pKa = 4.01DD1177 pKa = 3.82TLALTTEE1184 pKa = 4.29TTADD1188 pKa = 3.01GSYY1191 pKa = 11.35AFGTVDD1197 pKa = 2.77STLTYY1202 pKa = 10.11RR1203 pKa = 11.84IEE1205 pKa = 4.13VDD1207 pKa = 3.21TTDD1210 pKa = 3.59PEE1212 pKa = 4.39IPAGLTLSTTNPLTGVAVTTGAEE1235 pKa = 4.15TTDD1238 pKa = 3.14QDD1240 pKa = 4.16FGFVAAPTAADD1251 pKa = 3.76LSLSKK1256 pKa = 10.83SVVSAANGLPVTQATAGEE1274 pKa = 4.19ALDD1277 pKa = 5.45FILTVTNDD1285 pKa = 3.5GPGTPTGVQVRR1296 pKa = 11.84DD1297 pKa = 3.54LMPQGFAYY1305 pKa = 10.79VSDD1308 pKa = 4.05DD1309 pKa = 4.19AGAQGDD1315 pKa = 4.22TYY1317 pKa = 11.29DD1318 pKa = 3.71TGTGIWALGDD1328 pKa = 3.53VPAGSNQTLTIRR1340 pKa = 11.84VTMRR1344 pKa = 11.84DD1345 pKa = 3.4SGEE1348 pKa = 4.06HH1349 pKa = 5.74TNTAEE1354 pKa = 4.16IVASSLPDD1362 pKa = 4.22PDD1364 pKa = 4.09SDD1366 pKa = 4.04PAVGALVDD1374 pKa = 4.4DD1375 pKa = 5.2LSDD1378 pKa = 3.75GLADD1382 pKa = 4.55DD1383 pKa = 5.75DD1384 pKa = 4.41EE1385 pKa = 6.96ASVTLAFTGTGATLSGVVFLDD1406 pKa = 3.35NGAGATAYY1414 pKa = 10.61DD1415 pKa = 4.39GVQEE1419 pKa = 4.13GAEE1422 pKa = 4.2AGTDD1426 pKa = 3.15RR1427 pKa = 11.84AVVAVYY1433 pKa = 10.33DD1434 pKa = 3.78SAGALIGSPAVAADD1448 pKa = 4.28GSWSLTLPDD1457 pKa = 4.94GYY1459 pKa = 9.51TDD1461 pKa = 3.52AVTVSVLPDD1470 pKa = 3.3AGLRR1474 pKa = 11.84AVSEE1478 pKa = 4.23TAAALPGLVNTDD1490 pKa = 3.2PRR1492 pKa = 11.84DD1493 pKa = 3.4GSFTFTPAAGTSYY1506 pKa = 11.45SDD1508 pKa = 3.58LSFGLIVEE1516 pKa = 4.42ARR1518 pKa = 11.84LNLDD1522 pKa = 2.82QQAAIRR1528 pKa = 11.84PGQVVSLRR1536 pKa = 11.84HH1537 pKa = 6.0EE1538 pKa = 4.37YY1539 pKa = 10.63VADD1542 pKa = 3.53ATGSVSFIVEE1552 pKa = 4.32NQTSATPGAFSTGLFLDD1569 pKa = 4.26TDD1571 pKa = 4.37CDD1573 pKa = 3.83GTANTPITDD1582 pKa = 3.83PVAIQAATLLCIVTRR1597 pKa = 11.84VSASSAVSPGASYY1610 pKa = 11.38SFDD1613 pKa = 3.44LVADD1617 pKa = 4.17TSYY1620 pKa = 11.3GASGLNEE1627 pKa = 3.74QDD1629 pKa = 3.51RR1630 pKa = 11.84NTDD1633 pKa = 3.11RR1634 pKa = 11.84VTVEE1638 pKa = 3.95SSQGALKK1645 pKa = 9.11LTKK1648 pKa = 8.82TVRR1651 pKa = 11.84NVTQGSAEE1659 pKa = 4.11GVANAAAAGDD1669 pKa = 3.65VLEE1672 pKa = 4.0YY1673 pKa = 10.43RR1674 pKa = 11.84IYY1676 pKa = 10.03VQNAGTLPSSDD1687 pKa = 3.12IIIYY1691 pKa = 10.55DD1692 pKa = 3.45RR1693 pKa = 11.84TPPYY1697 pKa = 9.87TVLANAVPSPVDD1709 pKa = 3.46LGGGVTCISSLPGTDD1724 pKa = 3.01SSGYY1728 pKa = 10.94AGDD1731 pKa = 5.12LRR1733 pKa = 11.84WNCTGSYY1740 pKa = 10.15QPGADD1745 pKa = 3.12GSVTFQIRR1753 pKa = 11.84ISPP1756 pKa = 3.72

Molecular weight: 180.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238XCW9|A0A238XCW9_9RHOB Serine-glyoxylate aminotransferase apoenzyme OS=Puniceibacterium sediminis OX=1608407 GN=SAMN06265370_110101 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.12RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 8.83HH15 pKa = 4.64RR16 pKa = 11.84HH17 pKa = 3.91GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4262

0

4262

1366683

28

8578

320.7

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.027 ± 0.054

0.91 ± 0.014

6.054 ± 0.038

5.495 ± 0.036

3.706 ± 0.028

8.685 ± 0.046

2.049 ± 0.021

5.244 ± 0.028

3.107 ± 0.035

10.23 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.024

2.631 ± 0.026

5.082 ± 0.033

3.285 ± 0.021

6.584 ± 0.045

5.472 ± 0.034

5.744 ± 0.042

7.338 ± 0.037

1.356 ± 0.016

2.203 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski