Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma gallisepticum; Mycoplasma gallisepticum str. R

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q7NBG0|SYI_MYCGA Isoleucine--tRNA ligase OS=Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) OX=710127 GN=ileS PE=3 SV=2
MM1 pKa = 8.26DD2 pKa = 4.84NNQNNFNQPGQQGFDD17 pKa = 3.05QYY19 pKa = 10.82QQQSGALVSYY29 pKa = 10.5GYY31 pKa = 9.95DD32 pKa = 3.17ANGNPVSDD40 pKa = 4.58PSLAVYY46 pKa = 9.34DD47 pKa = 4.24ANGMLINQNQYY58 pKa = 10.8DD59 pKa = 3.67QNQQQEE65 pKa = 4.14YY66 pKa = 9.14DD67 pKa = 3.32QYY69 pKa = 10.47GNPVGMLSGNVYY81 pKa = 10.34SQEE84 pKa = 3.76NDD86 pKa = 3.23PYY88 pKa = 8.68YY89 pKa = 10.26QQYY92 pKa = 9.78NQQQNQGYY100 pKa = 7.05EE101 pKa = 3.97QQYY104 pKa = 11.21DD105 pKa = 3.83EE106 pKa = 4.56YY107 pKa = 10.56GNPIGLLPGSEE118 pKa = 3.9NQQNNQQQYY127 pKa = 9.11DD128 pKa = 3.58QYY130 pKa = 10.77GNPIGMLPGGTNDD143 pKa = 3.45QAYY146 pKa = 10.43DD147 pKa = 4.11PNQMQYY153 pKa = 10.87DD154 pKa = 3.78QYY156 pKa = 10.59GNPVGMLAGVNANDD170 pKa = 3.64QGYY173 pKa = 9.85DD174 pKa = 3.86YY175 pKa = 11.62NQMQYY180 pKa = 11.14DD181 pKa = 4.1EE182 pKa = 4.67YY183 pKa = 10.41GNPVGMLPGGNANDD197 pKa = 3.28QGYY200 pKa = 9.94DD201 pKa = 3.86YY202 pKa = 11.62NQMQYY207 pKa = 11.1DD208 pKa = 4.18EE209 pKa = 4.97YY210 pKa = 11.03GNPPALYY217 pKa = 10.54DD218 pKa = 4.02NNQQDD223 pKa = 4.6YY224 pKa = 11.05YY225 pKa = 11.45GYY227 pKa = 10.49DD228 pKa = 3.01QNQQYY233 pKa = 10.85DD234 pKa = 3.41QANNQLAVVDD244 pKa = 4.29EE245 pKa = 4.96NYY247 pKa = 10.36EE248 pKa = 3.79QEE250 pKa = 4.26QQVEE254 pKa = 4.68SNEE257 pKa = 4.18EE258 pKa = 3.73PAHH261 pKa = 5.19EE262 pKa = 3.88QDD264 pKa = 5.39LRR266 pKa = 11.84EE267 pKa = 3.99FLNNNSDD274 pKa = 4.01TEE276 pKa = 4.22LVSYY280 pKa = 10.65YY281 pKa = 10.34EE282 pKa = 4.11EE283 pKa = 4.74EE284 pKa = 4.09EE285 pKa = 4.48DD286 pKa = 4.49EE287 pKa = 4.32KK288 pKa = 11.4KK289 pKa = 10.43PRR291 pKa = 11.84NKK293 pKa = 9.98KK294 pKa = 9.06KK295 pKa = 10.25QRR297 pKa = 11.84QTAQARR303 pKa = 11.84GLLPEE308 pKa = 4.95LATVNQPDD316 pKa = 3.61QTPITPHH323 pKa = 7.34LEE325 pKa = 4.24APVHH329 pKa = 5.6YY330 pKa = 10.21DD331 pKa = 3.21EE332 pKa = 6.05NEE334 pKa = 3.94EE335 pKa = 4.94LSTDD339 pKa = 4.65DD340 pKa = 5.98LSDD343 pKa = 6.31DD344 pKa = 4.1INLDD348 pKa = 3.28QNQAVHH354 pKa = 6.97HH355 pKa = 6.64DD356 pKa = 3.99AEE358 pKa = 6.21DD359 pKa = 3.65DD360 pKa = 4.16VINIPIEE367 pKa = 4.76DD368 pKa = 4.13IEE370 pKa = 4.58SALLPKK376 pKa = 10.39FEE378 pKa = 4.97EE379 pKa = 4.23IQRR382 pKa = 11.84HH383 pKa = 4.53NLEE386 pKa = 4.76EE387 pKa = 3.95IQKK390 pKa = 9.72VKK392 pKa = 11.11LEE394 pKa = 3.85AAEE397 pKa = 4.03NFKK400 pKa = 10.83VLQRR404 pKa = 11.84ANEE407 pKa = 4.0EE408 pKa = 4.15LKK410 pKa = 11.1SSNNEE415 pKa = 3.71LKK417 pKa = 8.11TTNQQLKK424 pKa = 8.71TSNEE428 pKa = 3.76ALEE431 pKa = 4.82DD432 pKa = 3.62SNKK435 pKa = 10.39RR436 pKa = 11.84IEE438 pKa = 4.44SQLQALLDD446 pKa = 4.33SINDD450 pKa = 3.26IKK452 pKa = 11.31SKK454 pKa = 10.94NNQPSQEE461 pKa = 4.21EE462 pKa = 3.86VDD464 pKa = 3.75SRR466 pKa = 11.84SKK468 pKa = 11.08LEE470 pKa = 3.75QRR472 pKa = 11.84LEE474 pKa = 3.84EE475 pKa = 4.04LAYY478 pKa = 10.01KK479 pKa = 10.42LDD481 pKa = 3.65QTKK484 pKa = 10.6EE485 pKa = 4.15IIDD488 pKa = 3.78EE489 pKa = 4.2TSEE492 pKa = 3.95SSRR495 pKa = 11.84EE496 pKa = 3.93SFQQSKK502 pKa = 10.71DD503 pKa = 3.33QLIEE507 pKa = 3.92NFEE510 pKa = 4.28KK511 pKa = 10.56KK512 pKa = 9.89IEE514 pKa = 4.1LLTEE518 pKa = 4.22KK519 pKa = 10.58LNQTQEE525 pKa = 4.26SFNQSQEE532 pKa = 4.05AKK534 pKa = 9.45QQQDD538 pKa = 2.98KK539 pKa = 11.07EE540 pKa = 4.3FAQKK544 pKa = 9.76IEE546 pKa = 4.5RR547 pKa = 11.84IIEE550 pKa = 3.96QTKK553 pKa = 9.33EE554 pKa = 4.17ANDD557 pKa = 3.6SLQNRR562 pKa = 11.84VKK564 pKa = 10.61EE565 pKa = 4.21SSLDD569 pKa = 3.66MEE571 pKa = 5.11SKK573 pKa = 10.86LEE575 pKa = 3.94NKK577 pKa = 9.99FEE579 pKa = 4.2SFADD583 pKa = 3.4KK584 pKa = 9.89LTEE587 pKa = 3.86ITSKK591 pKa = 10.7KK592 pKa = 9.49ISEE595 pKa = 4.4KK596 pKa = 8.46MTEE599 pKa = 3.88QQASKK604 pKa = 10.76KK605 pKa = 10.35EE606 pKa = 4.26EE607 pKa = 3.96IDD609 pKa = 3.6SLQSSFQKK617 pKa = 10.82ALSEE621 pKa = 4.23VVSKK625 pKa = 11.11VEE627 pKa = 3.9NYY629 pKa = 10.9ANQSQLQSQHH639 pKa = 6.2LNQTLSYY646 pKa = 10.03HH647 pKa = 4.97QQQINNSLRR656 pKa = 11.84QSAQMAQMAQMQHH669 pKa = 5.81MMPQQMLMGMNNPYY683 pKa = 10.42GFNHH687 pKa = 6.37HH688 pKa = 5.78TMMPPVHH695 pKa = 6.64QYY697 pKa = 9.57QQLPPPPPVQAPAQQLLPTINNPLQLPNEE726 pKa = 4.14NPTLFEE732 pKa = 4.8KK733 pKa = 11.01LMLANMFKK741 pKa = 9.22QTINPPQPQPQALPQPHH758 pKa = 7.1PQPQQLPPQILALPPTVVQQPNYY781 pKa = 10.26LVPQPPRR788 pKa = 11.84QPDD791 pKa = 4.02YY792 pKa = 11.1YY793 pKa = 11.06SNRR796 pKa = 11.84LNEE799 pKa = 5.01RR800 pKa = 11.84MMLDD804 pKa = 3.4DD805 pKa = 5.83AYY807 pKa = 11.52NAGYY811 pKa = 10.66DD812 pKa = 3.48EE813 pKa = 5.26AVYY816 pKa = 10.29EE817 pKa = 4.93LEE819 pKa = 4.07NQYY822 pKa = 10.67YY823 pKa = 8.58PPAYY827 pKa = 9.21EE828 pKa = 3.85YY829 pKa = 10.43PEE831 pKa = 4.15YY832 pKa = 11.16EE833 pKa = 4.23EE834 pKa = 4.28IQPSFRR840 pKa = 11.84RR841 pKa = 11.84RR842 pKa = 11.84GGRR845 pKa = 11.84AKK847 pKa = 10.39FDD849 pKa = 3.83PYY851 pKa = 11.54NNRR854 pKa = 3.4

Molecular weight: 98.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8WJY3|F8WJY3_MYCGA High affinity transport system protein OS=Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) OX=710127 GN=hatA PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84PAPSPMGSPKK12 pKa = 10.69LLGPNQAGHH21 pKa = 6.37PQHH24 pKa = 6.8GPRR27 pKa = 11.84PMNAHH32 pKa = 7.34PGQPRR37 pKa = 11.84PQQAGPRR44 pKa = 11.84PMGAGGSNQPRR55 pKa = 11.84PMPNGLQNPQGPRR68 pKa = 11.84PMNPQGDD75 pKa = 4.49PRR77 pKa = 11.84PQPAGVRR84 pKa = 11.84PNSPQNSQPRR94 pKa = 11.84PMPNKK99 pKa = 8.94PQGPRR104 pKa = 11.84PMGAPNPQPGPQQAGPRR121 pKa = 11.84PMGVGGSNQPRR132 pKa = 11.84PMPNGLQNPQGPRR145 pKa = 11.84PMNPQGDD152 pKa = 4.49PRR154 pKa = 11.84PQPAGVRR161 pKa = 11.84PNSPQANQPGRR172 pKa = 11.84RR173 pKa = 11.84PTPNNPQGPRR183 pKa = 11.84PMGPRR188 pKa = 11.84PNGGPNRR195 pKa = 11.84AA196 pKa = 3.81

Molecular weight: 20.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

761

0

761

295319

37

1969

388.1

44.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.855 ± 0.087

0.519 ± 0.021

5.679 ± 0.068

5.605 ± 0.146

4.925 ± 0.081

4.675 ± 0.082

1.437 ± 0.039

8.244 ± 0.118

8.903 ± 0.108

9.789 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.036

8.033 ± 0.105

3.255 ± 0.062

4.213 ± 0.095

3.159 ± 0.049

7.119 ± 0.083

5.828 ± 0.109

5.912 ± 0.068

0.869 ± 0.024

4.293 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski