Bacteriophage Phobos

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2U981|A0A5Q2U981_9CAUD Uncharacterized protein OS=Bacteriophage Phobos OX=2662138 PE=4 SV=1
MM1 pKa = 7.04TTTKK5 pKa = 9.1QTFTAIRR12 pKa = 11.84SAMARR17 pKa = 11.84AFFANAYY24 pKa = 9.98ADD26 pKa = 3.65QAEE29 pKa = 4.63EE30 pKa = 3.96ALQPLGGEE38 pKa = 4.62ILDD41 pKa = 4.03QLPDD45 pKa = 3.87VIDD48 pKa = 4.47PNAEE52 pKa = 3.64AAALEE57 pKa = 4.33LCEE60 pKa = 4.85KK61 pKa = 10.68LADD64 pKa = 3.4QFDD67 pKa = 4.3YY68 pKa = 11.58NEE70 pKa = 4.86RR71 pKa = 11.84GLSLAQKK78 pKa = 10.2VALLFLKK85 pKa = 10.47ACRR88 pKa = 11.84ADD90 pKa = 3.4FANRR94 pKa = 11.84DD95 pKa = 3.59RR96 pKa = 11.84EE97 pKa = 4.31LTAEE101 pKa = 4.1NFGHH105 pKa = 5.89YY106 pKa = 10.35LAMQAMGTGVGLEE119 pKa = 4.09AFGSDD124 pKa = 3.31VRR126 pKa = 11.84DD127 pKa = 4.86AIPVPYY133 pKa = 10.6LEE135 pKa = 5.23FGSAHH140 pKa = 7.12LSQEE144 pKa = 3.9YY145 pKa = 10.22FLADD149 pKa = 3.4VADD152 pKa = 4.37DD153 pKa = 3.67

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2U8B3|A0A5Q2U8B3_9CAUD Uncharacterized protein OS=Bacteriophage Phobos OX=2662138 PE=4 SV=1
MM1 pKa = 6.41QQDD4 pKa = 3.78YY5 pKa = 10.14VVAVAGLSSLDD16 pKa = 4.63DD17 pKa = 3.65INNLDD22 pKa = 3.63EE23 pKa = 6.07AIIANARR30 pKa = 11.84MAINRR35 pKa = 11.84TVDD38 pKa = 3.22RR39 pKa = 11.84ARR41 pKa = 11.84TASAKK46 pKa = 9.25EE47 pKa = 3.8MRR49 pKa = 11.84DD50 pKa = 3.3QIAFPARR57 pKa = 11.84YY58 pKa = 9.43LSGTNGRR65 pKa = 11.84LRR67 pKa = 11.84VSEE70 pKa = 4.23RR71 pKa = 11.84ATNTSLQATITGRR84 pKa = 11.84DD85 pKa = 3.38RR86 pKa = 11.84PTSLARR92 pKa = 11.84FATSRR97 pKa = 11.84DD98 pKa = 3.23PAATRR103 pKa = 11.84RR104 pKa = 11.84QGGVRR109 pKa = 11.84VTVDD113 pKa = 3.07PGKK116 pKa = 10.62SKK118 pKa = 10.39FMRR121 pKa = 11.84GAFLMRR127 pKa = 11.84LNNNNLGLAIRR138 pKa = 11.84LKK140 pKa = 10.19EE141 pKa = 3.98GEE143 pKa = 4.08RR144 pKa = 11.84VVNKK148 pKa = 9.47TRR150 pKa = 11.84MTRR153 pKa = 11.84IGAGLYY159 pKa = 10.23LLYY162 pKa = 10.56GPSVDD167 pKa = 3.52QVFRR171 pKa = 11.84SVADD175 pKa = 3.74DD176 pKa = 3.85QAPKK180 pKa = 10.57AADD183 pKa = 3.48FLEE186 pKa = 5.37AEE188 pKa = 4.85FLRR191 pKa = 11.84LMEE194 pKa = 4.43LL195 pKa = 3.6

Molecular weight: 21.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

17398

38

1727

276.2

30.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.817 ± 0.64

0.902 ± 0.133

6.673 ± 0.38

6.616 ± 0.248

3.518 ± 0.181

7.443 ± 0.284

1.903 ± 0.199

4.248 ± 0.159

4.88 ± 0.342

8.921 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.116

3.679 ± 0.155

4.978 ± 0.468

4.219 ± 0.449

6.42 ± 0.267

4.845 ± 0.249

6.041 ± 0.273

6.673 ± 0.216

1.782 ± 0.221

2.707 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski