Rhodococcus phage Trina

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Trinavirus; Rhodococcus virus Trina

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0ZN26|A0A2D0ZN26_9CAUD Uncharacterized protein OS=Rhodococcus phage Trina OX=2027905 GN=SEA_TRINA_171 PE=4 SV=1
MM1 pKa = 7.72PEE3 pKa = 3.7EE4 pKa = 4.1TVTISKK10 pKa = 8.97QQYY13 pKa = 9.16DD14 pKa = 4.05SLVEE18 pKa = 4.12DD19 pKa = 3.9SEE21 pKa = 4.38FLQRR25 pKa = 11.84LQDD28 pKa = 3.68GGVDD32 pKa = 3.39NWPGYY37 pKa = 8.94SDD39 pKa = 5.81CYY41 pKa = 8.39TQEE44 pKa = 3.84KK45 pKa = 10.25FEE47 pKa = 4.27AAFGG51 pKa = 3.71

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0ZMB5|A0A2D0ZMB5_9CAUD Uncharacterized protein OS=Rhodococcus phage Trina OX=2027905 GN=SEA_TRINA_246 PE=4 SV=1
MM1 pKa = 7.49SNDD4 pKa = 3.12EE5 pKa = 4.5RR6 pKa = 11.84VGLLKK11 pKa = 10.11WRR13 pKa = 11.84EE14 pKa = 3.66RR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 10.53AEE19 pKa = 3.68SSARR23 pKa = 11.84KK24 pKa = 8.63PRR26 pKa = 11.84NKK28 pKa = 10.21AKK30 pKa = 10.57YY31 pKa = 9.44SRR33 pKa = 11.84EE34 pKa = 4.1DD35 pKa = 3.3YY36 pKa = 10.5RR37 pKa = 11.84KK38 pKa = 9.46EE39 pKa = 3.79SQRR42 pKa = 11.84EE43 pKa = 3.99LKK45 pKa = 10.58HH46 pKa = 5.88RR47 pKa = 4.0

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

250

0

250

41044

29

2380

164.2

18.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.184 ± 0.418

0.872 ± 0.084

6.544 ± 0.149

6.469 ± 0.201

3.913 ± 0.143

6.802 ± 0.231

1.832 ± 0.112

6.115 ± 0.125

6.162 ± 0.187

7.716 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.111

5.141 ± 0.143

3.735 ± 0.171

3.755 ± 0.206

5.017 ± 0.124

6.408 ± 0.152

6.279 ± 0.255

6.729 ± 0.175

1.754 ± 0.094

3.942 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski