Mesoplasma syrphidae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Mesoplasma

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9CA01|A0A2K9CA01_9MOLU Thioredoxin reductase OS=Mesoplasma syrphidae OX=225999 GN=trxB PE=3 SV=1
MM1 pKa = 7.64EE2 pKa = 4.66FMEE5 pKa = 5.85LIAAAQSCEE14 pKa = 3.71TSIIEE19 pKa = 4.94IINSDD24 pKa = 3.77EE25 pKa = 3.95QSEE28 pKa = 4.9TEE30 pKa = 4.1FKK32 pKa = 10.68EE33 pKa = 3.99FAIEE37 pKa = 4.06EE38 pKa = 4.33VQHH41 pKa = 6.51LATLFKK47 pKa = 10.43TFLVEE52 pKa = 3.88LDD54 pKa = 3.65KK55 pKa = 11.58KK56 pKa = 10.66EE57 pKa = 4.19EE58 pKa = 4.27SVDD61 pKa = 3.8TLWFSASKK69 pKa = 10.44LVYY72 pKa = 9.96EE73 pKa = 4.47YY74 pKa = 10.8IEE76 pKa = 4.83FEE78 pKa = 4.14TKK80 pKa = 10.58YY81 pKa = 10.26EE82 pKa = 3.99LEE84 pKa = 4.13EE85 pKa = 4.32DD86 pKa = 4.06VEE88 pKa = 4.78LEE90 pKa = 4.1TIEE93 pKa = 4.08DD94 pKa = 3.5VKK96 pKa = 10.9IFRR99 pKa = 11.84FQVLEE104 pKa = 4.21FFSSFFDD111 pKa = 3.45EE112 pKa = 6.54SEE114 pKa = 5.05DD115 pKa = 4.11LDD117 pKa = 4.14QNVV120 pKa = 3.06

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9BPH4|A0A2K9BPH4_9MOLU Membrane protein insertase YidC OS=Mesoplasma syrphidae OX=225999 GN=CXP39_03930 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.46IKK11 pKa = 9.18TARR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.12NGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 10.29ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

740

0

740

269842

37

1783

364.7

41.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.215 ± 0.08

0.577 ± 0.021

5.112 ± 0.08

6.846 ± 0.099

5.144 ± 0.077

5.527 ± 0.083

1.304 ± 0.032

9.636 ± 0.087

9.112 ± 0.086

9.236 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.037

6.73 ± 0.085

2.608 ± 0.04

3.578 ± 0.049

3.094 ± 0.06

6.319 ± 0.07

5.55 ± 0.054

6.158 ± 0.07

1.098 ± 0.035

3.839 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski