Mycobacterium phage LilSpotty

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EM27|A0A4Y6EM27_9CAUD Tail terminator OS=Mycobacterium phage LilSpotty OX=2588512 GN=11 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.28LDD5 pKa = 3.88TLTDD9 pKa = 3.76DD10 pKa = 3.74FTVYY14 pKa = 10.9DD15 pKa = 4.1PGKK18 pKa = 7.82WTIDD22 pKa = 3.45DD23 pKa = 4.19SVGGDD28 pKa = 3.05IDD30 pKa = 4.0IGDD33 pKa = 3.53GRR35 pKa = 11.84AAINTNAGIEE45 pKa = 4.51GVFSVGAYY53 pKa = 10.22DD54 pKa = 4.68LLEE57 pKa = 4.63SYY59 pKa = 11.22AFAQFISFGFLYY71 pKa = 9.7PGKK74 pKa = 10.79GFGFSLNINSGGPNEE89 pKa = 4.37DD90 pKa = 2.86SAMFLCTEE98 pKa = 4.28SEE100 pKa = 4.55TEE102 pKa = 3.85GEE104 pKa = 4.27YY105 pKa = 11.3NLICLDD111 pKa = 3.54GAGFGAEE118 pKa = 4.19GTPIEE123 pKa = 4.4PVATGVTYY131 pKa = 10.74DD132 pKa = 3.82PAIHH136 pKa = 5.58SWLRR140 pKa = 11.84IRR142 pKa = 11.84EE143 pKa = 4.28SGGTIYY149 pKa = 10.47WEE151 pKa = 4.34TSPDD155 pKa = 3.6TAPLEE160 pKa = 4.22WSVMASGAHH169 pKa = 5.76TFTSAQATVQLGGVFAPAAFTVSSFNIAPTPPIPANAGNFFAFFF213 pKa = 4.14

Molecular weight: 22.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EP19|A0A4Y6EP19_9CAUD Uncharacterized protein OS=Mycobacterium phage LilSpotty OX=2588512 GN=75 PE=4 SV=1
MM1 pKa = 7.65LISRR5 pKa = 11.84CRR7 pKa = 11.84TRR9 pKa = 11.84SLLTTRR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 9.6RR19 pKa = 11.84PEE21 pKa = 3.8PLAGAFVVPEE31 pKa = 4.08INKK34 pKa = 9.77APATASTAPGCGRR47 pKa = 11.84LQGVDD52 pKa = 3.45MADD55 pKa = 5.26DD56 pKa = 3.94IPTVAWKK63 pKa = 9.89SHH65 pKa = 6.32PEE67 pKa = 3.89YY68 pKa = 10.26PHH70 pKa = 6.01YY71 pKa = 10.25QASNDD76 pKa = 3.21GRR78 pKa = 11.84VRR80 pKa = 11.84TLDD83 pKa = 3.09RR84 pKa = 11.84MEE86 pKa = 4.21PQGARR91 pKa = 11.84GMCFLPGRR99 pKa = 11.84EE100 pKa = 4.08LSLFPIDD107 pKa = 4.7GYY109 pKa = 10.14LTFDD113 pKa = 2.81IKK115 pKa = 10.55KK116 pKa = 9.86RR117 pKa = 11.84KK118 pKa = 8.9VKK120 pKa = 8.42VHH122 pKa = 6.58RR123 pKa = 11.84FVCEE127 pKa = 4.07VFNGPPPSPTHH138 pKa = 5.68EE139 pKa = 3.87VRR141 pKa = 11.84HH142 pKa = 5.88LNGDD146 pKa = 3.42PRR148 pKa = 11.84DD149 pKa = 3.88NRR151 pKa = 11.84PEE153 pKa = 3.72NLAWGTAKK161 pKa = 10.44EE162 pKa = 4.09NATDD166 pKa = 3.74RR167 pKa = 11.84VRR169 pKa = 11.84HH170 pKa = 4.67GRR172 pKa = 11.84QYY174 pKa = 11.1RR175 pKa = 11.84GGKK178 pKa = 8.77AGRR181 pKa = 11.84RR182 pKa = 11.84VSSDD186 pKa = 2.84STHH189 pKa = 5.89CRR191 pKa = 11.84KK192 pKa = 9.78GHH194 pKa = 6.36EE195 pKa = 4.06YY196 pKa = 10.5NAANTAYY203 pKa = 10.29APDD206 pKa = 2.99GWRR209 pKa = 11.84RR210 pKa = 11.84CRR212 pKa = 11.84ACAADD217 pKa = 3.45EE218 pKa = 4.17TRR220 pKa = 11.84RR221 pKa = 11.84RR222 pKa = 11.84RR223 pKa = 11.84ARR225 pKa = 11.84MRR227 pKa = 11.84EE228 pKa = 3.64RR229 pKa = 11.84AKK231 pKa = 10.66AASVV235 pKa = 3.13

Molecular weight: 26.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

15588

31

1395

183.4

20.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.875 ± 0.396

1.084 ± 0.163

6.742 ± 0.205

5.94 ± 0.298

2.829 ± 0.149

8.41 ± 0.428

2.002 ± 0.177

4.484 ± 0.185

3.349 ± 0.21

8.025 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.121

3.06 ± 0.233

5.819 ± 0.187

3.727 ± 0.221

7.121 ± 0.436

5.504 ± 0.219

6.248 ± 0.31

7.134 ± 0.255

2.277 ± 0.151

2.226 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski