Human immunodeficiency virus type 1 group N (isolate YBF30) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group N

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O91086|ENV_HV1YF Envelope glycoprotein gp160 OS=Human immunodeficiency virus type 1 group N (isolate YBF30) OX=388818 GN=env PE=3 SV=1
MM1 pKa = 7.59LSLGFIALGAAVSIAVIVWALLYY24 pKa = 10.39RR25 pKa = 11.84EE26 pKa = 4.05YY27 pKa = 11.29KK28 pKa = 10.14KK29 pKa = 10.7IKK31 pKa = 9.05LQEE34 pKa = 4.34KK35 pKa = 9.39IKK37 pKa = 10.42HH38 pKa = 4.36IRR40 pKa = 11.84QRR42 pKa = 11.84IRR44 pKa = 11.84EE45 pKa = 4.04RR46 pKa = 11.84EE47 pKa = 3.79EE48 pKa = 4.65DD49 pKa = 3.6SGNEE53 pKa = 3.89SDD55 pKa = 6.43GDD57 pKa = 4.22AEE59 pKa = 4.12WLDD62 pKa = 4.18GDD64 pKa = 4.47EE65 pKa = 4.29EE66 pKa = 4.55WLVTLLSSSKK76 pKa = 10.27LDD78 pKa = 3.28QGNWVV83 pKa = 3.35

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O91085|VPU_HV1YF Protein Vpu OS=Human immunodeficiency virus type 1 group N (isolate YBF30) OX=388818 GN=vpu PE=3 SV=1
MM1 pKa = 7.78AGRR4 pKa = 11.84SGVNDD9 pKa = 3.68EE10 pKa = 4.26EE11 pKa = 4.49LLRR14 pKa = 11.84AVRR17 pKa = 11.84VIKK20 pKa = 10.26ILYY23 pKa = 8.96QSNPYY28 pKa = 9.29PNSKK32 pKa = 8.62GTRR35 pKa = 11.84QARR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WRR46 pKa = 11.84ARR48 pKa = 11.84QRR50 pKa = 11.84QIRR53 pKa = 11.84AISEE57 pKa = 4.62RR58 pKa = 11.84ILSSCLGGPPEE69 pKa = 4.65PVDD72 pKa = 5.03LPLPPLDD79 pKa = 4.75RR80 pKa = 11.84LTLDD84 pKa = 3.49TEE86 pKa = 4.47EE87 pKa = 5.59DD88 pKa = 3.58SGTPGTEE95 pKa = 4.15SQQGTATTEE104 pKa = 3.7

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3662

72

1449

366.2

41.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.445 ± 0.587

2.021 ± 0.418

3.932 ± 0.428

7.373 ± 0.405

2.348 ± 0.275

7.428 ± 0.144

2.376 ± 0.589

6.117 ± 0.903

6.636 ± 0.946

8.629 ± 0.654

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.271

4.533 ± 0.529

5.762 ± 0.725

5.735 ± 0.36

6.117 ± 0.998

4.888 ± 0.564

5.953 ± 0.463

6.035 ± 0.606

2.703 ± 0.338

2.84 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski