Streptomyces sp. NRRL S-1521

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X3W472|A0A0X3W472_9ACTN Uncharacterized protein OS=Streptomyces sp. NRRL S-1521 OX=1609100 GN=ADL30_27085 PE=4 SV=1
MM1 pKa = 7.04TVRR4 pKa = 11.84HH5 pKa = 5.26EE6 pKa = 4.48AEE8 pKa = 4.31TGSEE12 pKa = 4.01GHH14 pKa = 7.14DD15 pKa = 3.94LLEE18 pKa = 3.98VWIDD22 pKa = 3.41QDD24 pKa = 3.99LCTGDD29 pKa = 5.01GICAQYY35 pKa = 10.9APEE38 pKa = 4.23VFEE41 pKa = 5.9LDD43 pKa = 3.05IDD45 pKa = 3.63GLAYY49 pKa = 10.47VKK51 pKa = 10.9SGDD54 pKa = 4.51DD55 pKa = 4.0EE56 pKa = 5.36LLQTPGATTPVPLPLLRR73 pKa = 11.84DD74 pKa = 3.6VADD77 pKa = 3.92SAKK80 pKa = 9.94EE81 pKa = 3.91CPGDD85 pKa = 4.18CIHH88 pKa = 6.42VRR90 pKa = 11.84RR91 pKa = 11.84VSDD94 pKa = 3.33GTEE97 pKa = 3.95VYY99 pKa = 10.88GPDD102 pKa = 3.74AEE104 pKa = 4.31

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X3W7W0|A0A0X3W7W0_9ACTN 3-hydroxyacyl-CoA dehydrogenase OS=Streptomyces sp. NRRL S-1521 OX=1609100 GN=ADL30_21665 PE=4 SV=1
MM1 pKa = 7.94PLTFRR6 pKa = 11.84KK7 pKa = 9.63SFRR10 pKa = 11.84ILPGVRR16 pKa = 11.84LNINKK21 pKa = 9.84RR22 pKa = 11.84SWSLTTGGRR31 pKa = 11.84HH32 pKa = 5.25GPRR35 pKa = 11.84RR36 pKa = 11.84THH38 pKa = 6.33SSTGRR43 pKa = 11.84RR44 pKa = 11.84TTSMDD49 pKa = 3.23LPGPFGWRR57 pKa = 11.84RR58 pKa = 11.84TTRR61 pKa = 11.84RR62 pKa = 11.84GGRR65 pKa = 11.84RR66 pKa = 3.2

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6869

0

6869

2333221

37

7851

339.7

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.908 ± 0.044

0.794 ± 0.009

6.115 ± 0.021

5.859 ± 0.028

2.717 ± 0.016

9.567 ± 0.026

2.367 ± 0.013

3.04 ± 0.023

2.277 ± 0.029

10.342 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.757 ± 0.014

1.637 ± 0.015

6.062 ± 0.031

2.614 ± 0.017

8.219 ± 0.035

4.9 ± 0.022

5.907 ± 0.019

8.41 ± 0.026

1.475 ± 0.011

2.034 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski