Wenzhou crab virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFJ5|A0A1L3KFJ5_9VIRU RdRP_1 domain-containing protein OS=Wenzhou crab virus 5 OX=1923562 PE=4 SV=1
MM1 pKa = 7.52LARR4 pKa = 11.84ARR6 pKa = 11.84MMTPQKK12 pKa = 10.6LAVCLPVGQSLYY24 pKa = 10.56GWYY27 pKa = 10.63SSMLAVSWGTHH38 pKa = 4.84LRR40 pKa = 11.84RR41 pKa = 11.84WPQNSGGPPFYY52 pKa = 10.6LRR54 pKa = 11.84TMTLEE59 pKa = 4.26PSSAIYY65 pKa = 9.88TMSRR69 pKa = 11.84YY70 pKa = 8.4FVCSSDD76 pKa = 3.75EE77 pKa = 4.21EE78 pKa = 4.39EE79 pKa = 5.37LNEE82 pKa = 4.63QYY84 pKa = 10.94EE85 pKa = 4.28LQLAGSSQSDD95 pKa = 3.45ANSCAEE101 pKa = 3.69GSTAGFCGLQSVSAQSVPDD120 pKa = 3.62VDD122 pKa = 4.18SSEE125 pKa = 4.13PACVCRR131 pKa = 11.84CFSTPHH137 pKa = 6.73GPTCYY142 pKa = 8.85VTRR145 pKa = 11.84GGVRR149 pKa = 11.84ALVEE153 pKa = 3.86ATAGIFSEE161 pKa = 4.23HH162 pKa = 6.32TGRR165 pKa = 11.84STRR168 pKa = 11.84TRR170 pKa = 11.84VILGALGATVLGAAAGAVTYY190 pKa = 10.65YY191 pKa = 11.26SLTTSGGTDD200 pKa = 3.05SLSEE204 pKa = 4.2SGRR207 pKa = 11.84EE208 pKa = 3.96ALGDD212 pKa = 3.52AVAQVSLNDD221 pKa = 3.75LPIVHH226 pKa = 7.35PEE228 pKa = 3.96STSPPSADD236 pKa = 3.08GSEE239 pKa = 3.96VSAWDD244 pKa = 3.62NQYY247 pKa = 11.51DD248 pKa = 3.41

Molecular weight: 26.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFJ5|A0A1L3KFJ5_9VIRU RdRP_1 domain-containing protein OS=Wenzhou crab virus 5 OX=1923562 PE=4 SV=1
MM1 pKa = 6.57YY2 pKa = 9.73TPGLSLGTTLARR14 pKa = 11.84LALSGPRR21 pKa = 11.84MQPIPNHH28 pKa = 5.61MSGDD32 pKa = 3.97EE33 pKa = 4.29DD34 pKa = 3.83SLSQLFEE41 pKa = 4.0RR42 pKa = 11.84LKK44 pKa = 11.26LIVTQPALIPEE55 pKa = 4.64LAGDD59 pKa = 3.81YY60 pKa = 10.82VPAGVQQLFEE70 pKa = 4.22QMRR73 pKa = 11.84GDD75 pKa = 3.34AALPRR80 pKa = 11.84WFYY83 pKa = 11.25QHH85 pKa = 6.85LSAPRR90 pKa = 11.84ALSLRR95 pKa = 11.84PGTQRR100 pKa = 11.84PTCQGMDD107 pKa = 4.1RR108 pKa = 11.84ITGLPEE114 pKa = 4.89AGGLPIPVFRR124 pKa = 11.84AVAEE128 pKa = 4.51EE129 pKa = 3.56IRR131 pKa = 11.84AEE133 pKa = 3.91RR134 pKa = 11.84RR135 pKa = 11.84QVGTDD140 pKa = 3.27PRR142 pKa = 11.84AVWFQEE148 pKa = 3.37KK149 pKa = 10.11HH150 pKa = 5.92RR151 pKa = 11.84LHH153 pKa = 6.99LPLTKK158 pKa = 10.45DD159 pKa = 2.89EE160 pKa = 5.08LGRR163 pKa = 11.84QRR165 pKa = 11.84YY166 pKa = 8.9IPTFRR171 pKa = 11.84WKK173 pKa = 10.14PDD175 pKa = 3.01VFGPRR180 pKa = 11.84FSLGIGMYY188 pKa = 10.45YY189 pKa = 10.31EE190 pKa = 4.38VLSHH194 pKa = 5.91EE195 pKa = 4.36QRR197 pKa = 11.84STLTSWLEE205 pKa = 3.66RR206 pKa = 11.84LEE208 pKa = 4.42PYY210 pKa = 9.96IALCQSTLTVVAIFKK225 pKa = 10.58AVEE228 pKa = 4.05GWAKK232 pKa = 10.61AAGDD236 pKa = 4.05LFKK239 pKa = 10.49HH240 pKa = 5.28WRR242 pKa = 11.84IPCGIALLGGYY253 pKa = 9.15QPAPPLVFFRR263 pKa = 11.84DD264 pKa = 4.03EE265 pKa = 3.91VSEE268 pKa = 4.28WVSGPLPDD276 pKa = 5.14IGQHH280 pKa = 5.26LPGWRR285 pKa = 11.84DD286 pKa = 3.27SFEE289 pKa = 4.14WASKK293 pKa = 10.59QLLTGCGPEE302 pKa = 3.97QPPDD306 pKa = 3.41TFEE309 pKa = 6.01DD310 pKa = 4.33YY311 pKa = 11.08VLRR314 pKa = 11.84GDD316 pKa = 3.7WVRR319 pKa = 11.84PGATDD324 pKa = 2.88QGTFPSVTSRR334 pKa = 11.84GEE336 pKa = 3.76IVSVRR341 pKa = 11.84KK342 pKa = 8.18TKK344 pKa = 10.77VAMFAATTPDD354 pKa = 3.82DD355 pKa = 4.68LLALAYY361 pKa = 10.65SPAQPPRR368 pKa = 11.84AMVKK372 pKa = 10.3RR373 pKa = 11.84EE374 pKa = 3.97LTKK377 pKa = 10.82ARR379 pKa = 11.84AVVITDD385 pKa = 3.38TPSYY389 pKa = 11.24LRR391 pKa = 11.84MSWISTALEE400 pKa = 3.72PLLRR404 pKa = 11.84RR405 pKa = 11.84SRR407 pKa = 11.84NASVFFSGKK416 pKa = 9.68RR417 pKa = 11.84LAKK420 pKa = 9.09MWADD424 pKa = 3.87LASDD428 pKa = 3.91SADD431 pKa = 3.2VTLWKK436 pKa = 10.77CPIDD440 pKa = 3.75QSKK443 pKa = 9.48FDD445 pKa = 4.09HH446 pKa = 6.32MVEE449 pKa = 4.22PWALSVILDD458 pKa = 4.4RR459 pKa = 11.84IEE461 pKa = 3.77EE462 pKa = 4.85LIMGDD467 pKa = 3.45QAKK470 pKa = 10.05HH471 pKa = 4.13VLRR474 pKa = 11.84LIRR477 pKa = 11.84RR478 pKa = 11.84DD479 pKa = 4.17LIPEE483 pKa = 4.32KK484 pKa = 10.55GTVTVGDD491 pKa = 3.67ADD493 pKa = 3.91VPVTKK498 pKa = 10.37GVLSGWRR505 pKa = 11.84WTALLDD511 pKa = 3.22TWVNFSQVVGSLHH524 pKa = 5.38YY525 pKa = 10.31LYY527 pKa = 10.46TRR529 pKa = 11.84GVVPSIDD536 pKa = 3.31YY537 pKa = 9.06TGWYY541 pKa = 8.94FQGDD545 pKa = 3.8DD546 pKa = 3.27ARR548 pKa = 11.84LVVRR552 pKa = 11.84NPGIAVAVAEE562 pKa = 4.39VMRR565 pKa = 11.84EE566 pKa = 4.01AGLKK570 pKa = 9.18IHH572 pKa = 7.17PSKK575 pKa = 9.81TWLSPFRR582 pKa = 11.84DD583 pKa = 3.29EE584 pKa = 5.52FLRR587 pKa = 11.84QVAEE591 pKa = 4.63DD592 pKa = 4.06NVTMGYY598 pKa = 10.01LARR601 pKa = 11.84SVPALLWRR609 pKa = 11.84NPINAAADD617 pKa = 3.5NWPAAARR624 pKa = 11.84AAVGRR629 pKa = 11.84WDD631 pKa = 3.58GLVLRR636 pKa = 11.84GADD639 pKa = 3.44PYY641 pKa = 11.08RR642 pKa = 11.84VLEE645 pKa = 4.24HH646 pKa = 6.72ALKK649 pKa = 10.42EE650 pKa = 4.12LTHH653 pKa = 6.92LIPYY657 pKa = 8.74QRR659 pKa = 11.84STIHH663 pKa = 6.3KK664 pKa = 8.35WLSLPPHH671 pKa = 6.74LGGAGVTWVTPDD683 pKa = 3.52LTSLALIRR691 pKa = 11.84YY692 pKa = 6.98PLEE695 pKa = 4.5RR696 pKa = 11.84SLLRR700 pKa = 11.84FDD702 pKa = 4.59GVGLFDD708 pKa = 4.37RR709 pKa = 11.84LVSQVDD715 pKa = 3.87LPRR718 pKa = 11.84DD719 pKa = 3.34EE720 pKa = 6.1LYY722 pKa = 10.96AKK724 pKa = 10.06CYY726 pKa = 10.13QYY728 pKa = 11.51LSDD731 pKa = 4.59VVDD734 pKa = 3.45THH736 pKa = 6.18EE737 pKa = 4.79PKK739 pKa = 10.4HH740 pKa = 6.18LYY742 pKa = 10.08RR743 pKa = 11.84DD744 pKa = 3.55ILGSMSKK751 pKa = 8.35PTLDD755 pKa = 2.62WGVPPKK761 pKa = 10.0PWPPLGLVPCGAPPAQPTWARR782 pKa = 11.84GLQFPNWFRR791 pKa = 11.84NIAIDD796 pKa = 3.47VAVRR800 pKa = 11.84TKK802 pKa = 9.01TEE804 pKa = 3.58KK805 pKa = 10.83SVDD808 pKa = 3.66VLFEE812 pKa = 4.71PYY814 pKa = 9.74SASHH818 pKa = 6.01SPIIRR823 pKa = 11.84ARR825 pKa = 11.84HH826 pKa = 5.29GRR828 pKa = 11.84NTWADD833 pKa = 3.3WAKK836 pKa = 10.73GALSLPVRR844 pKa = 11.84CWPGWDD850 pKa = 3.21KK851 pKa = 11.5GYY853 pKa = 10.63LATQYY858 pKa = 10.52RR859 pKa = 11.84ARR861 pKa = 11.84IDD863 pKa = 3.58TFVRR867 pKa = 11.84CYY869 pKa = 10.37LGSSRR874 pKa = 11.84RR875 pKa = 11.84WGSRR879 pKa = 11.84ARR881 pKa = 11.84LASEE885 pKa = 4.01VFFYY889 pKa = 10.79SIQRR893 pKa = 11.84CPDD896 pKa = 2.69SLYY899 pKa = 10.05PYY901 pKa = 10.08RR902 pKa = 11.84IAAA905 pKa = 3.95

Molecular weight: 101.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2974

248

938

594.8

64.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.323 ± 0.61

1.379 ± 0.41

5.077 ± 0.652

4.573 ± 0.583

2.488 ± 0.363

8.171 ± 0.524

2.085 ± 0.27

3.497 ± 0.475

2.757 ± 0.503

9.718 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.106

1.984 ± 0.405

8.272 ± 0.654

4.035 ± 0.343

6.691 ± 0.834

7.835 ± 1.151

6.153 ± 0.454

7.297 ± 0.377

2.656 ± 0.396

3.194 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski