Penicillium digitatum polymycoviruses 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus; Penicillium digitatum polymycovirus 1

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4SUG9|A0A2R4SUG9_9VIRU Uncharacterized protein OS=Penicillium digitatum polymycoviruses 1 OX=2164101 PE=4 SV=1
MM1 pKa = 7.68AGNLARR7 pKa = 11.84LRR9 pKa = 11.84SLVLSAHH16 pKa = 6.97ADD18 pKa = 3.67TLLVASTIFHH28 pKa = 6.89CFPDD32 pKa = 4.18PTAHH36 pKa = 6.98QDD38 pKa = 2.76ITRR41 pKa = 11.84PEE43 pKa = 3.7VEE45 pKa = 5.63AYY47 pKa = 9.07INWMASSPYY56 pKa = 8.21PAYY59 pKa = 10.33GRR61 pKa = 11.84VVGHH65 pKa = 6.01VIEE68 pKa = 4.34KK69 pKa = 10.3RR70 pKa = 11.84EE71 pKa = 3.86EE72 pKa = 3.9LVEE75 pKa = 3.84VFMRR79 pKa = 11.84LEE81 pKa = 3.88PEE83 pKa = 4.08RR84 pKa = 11.84YY85 pKa = 9.42EE86 pKa = 5.04DD87 pKa = 4.57VADD90 pKa = 4.3LVNAMSTADD99 pKa = 3.8AAWDD103 pKa = 3.5KK104 pKa = 10.89SARR107 pKa = 11.84ASATNDD113 pKa = 3.13LVRR116 pKa = 11.84NTASAAQLAGHH127 pKa = 5.98SAVEE131 pKa = 4.69FVALEE136 pKa = 4.2SLVSAGYY143 pKa = 10.93DD144 pKa = 3.45NISLATTSIDD154 pKa = 3.7LLRR157 pKa = 11.84KK158 pKa = 8.02TGFIDD163 pKa = 4.63RR164 pKa = 11.84MVDD167 pKa = 3.33DD168 pKa = 5.02GLQLRR173 pKa = 11.84RR174 pKa = 11.84SRR176 pKa = 11.84RR177 pKa = 11.84AVAASWVEE185 pKa = 3.78EE186 pKa = 3.93QTGVRR191 pKa = 11.84RR192 pKa = 11.84FFVSPWCLSKK202 pKa = 10.56QACLALLAHH211 pKa = 6.41YY212 pKa = 10.32VRR214 pKa = 11.84EE215 pKa = 4.15PNRR218 pKa = 11.84ARR220 pKa = 11.84LEE222 pKa = 4.06AVAVRR227 pKa = 11.84RR228 pKa = 11.84GQTALTYY235 pKa = 8.55EE236 pKa = 4.19ARR238 pKa = 11.84AGLQAVRR245 pKa = 11.84AILEE249 pKa = 3.79PAIAILHH256 pKa = 4.64SHH258 pKa = 7.03RR259 pKa = 11.84YY260 pKa = 9.8DD261 pKa = 3.71PLQLAFVDD269 pKa = 3.45RR270 pKa = 11.84HH271 pKa = 4.81GRR273 pKa = 11.84RR274 pKa = 11.84AYY276 pKa = 10.42DD277 pKa = 2.73IATRR281 pKa = 11.84TKK283 pKa = 9.18HH284 pKa = 5.17VLVAFSYY291 pKa = 6.61VHH293 pKa = 6.33CRR295 pKa = 11.84GSEE298 pKa = 3.93DD299 pKa = 3.5TLLEE303 pKa = 4.06LARR306 pKa = 11.84VRR308 pKa = 11.84SALAAPDD315 pKa = 3.12ITACRR320 pKa = 11.84EE321 pKa = 3.74YY322 pKa = 11.47AEE324 pKa = 4.58FRR326 pKa = 11.84DD327 pKa = 3.87AEE329 pKa = 4.4EE330 pKa = 3.98VDD332 pKa = 3.63EE333 pKa = 4.71TGLLVLFARR342 pKa = 11.84TLLATRR348 pKa = 11.84DD349 pKa = 3.54HH350 pKa = 7.19RR351 pKa = 11.84DD352 pKa = 3.6DD353 pKa = 3.56IGFGDD358 pKa = 5.48GYY360 pKa = 11.26NKK362 pKa = 9.84PPCLGLVMRR371 pKa = 11.84ARR373 pKa = 11.84GDD375 pKa = 3.48EE376 pKa = 3.97EE377 pKa = 5.68RR378 pKa = 11.84NTLACVARR386 pKa = 11.84CQEE389 pKa = 3.94TVAYY393 pKa = 9.79LRR395 pKa = 11.84HH396 pKa = 6.27RR397 pKa = 11.84EE398 pKa = 3.82VDD400 pKa = 3.2LGKK403 pKa = 10.25QLLAVHH409 pKa = 6.66WEE411 pKa = 4.06GDD413 pKa = 3.84LNPDD417 pKa = 3.38VVIAAMAVSKK427 pKa = 11.19VDD429 pKa = 3.15IAVDD433 pKa = 3.12IGTASVDD440 pKa = 3.26IPGAAPIGASDD451 pKa = 3.38SSYY454 pKa = 11.54LVLLASAEE462 pKa = 4.05ARR464 pKa = 11.84GLPRR468 pKa = 11.84IIRR471 pKa = 11.84LPYY474 pKa = 8.42PTGLSISDD482 pKa = 3.49RR483 pKa = 11.84VEE485 pKa = 3.77LLYY488 pKa = 10.83EE489 pKa = 4.84FYY491 pKa = 10.05TASGQGAVCYY501 pKa = 10.4VGGGSPFSGKK511 pKa = 9.69PSTQTCADD519 pKa = 3.71ANSVARR525 pKa = 11.84ALAPHH530 pKa = 6.23VLSGALTFGTADD542 pKa = 4.66FILPNLCAAHH552 pKa = 6.36ARR554 pKa = 11.84RR555 pKa = 11.84SEE557 pKa = 3.95VAHH560 pKa = 7.04AIEE563 pKa = 4.72CNGAFEE569 pKa = 4.59YY570 pKa = 10.12TCHH573 pKa = 6.19NCVGFAHH580 pKa = 7.53GLRR583 pKa = 11.84DD584 pKa = 3.65TVEE587 pKa = 4.08LVGMAGARR595 pKa = 11.84LVKK598 pKa = 9.65TRR600 pKa = 11.84SAYY603 pKa = 9.65AHH605 pKa = 6.37NGNFSVEE612 pKa = 4.03LFGAGDD618 pKa = 4.11PDD620 pKa = 5.09FEE622 pKa = 4.9DD623 pKa = 3.71TVQTIEE629 pKa = 3.92TTAFMTAVRR638 pKa = 11.84NADD641 pKa = 3.22WEE643 pKa = 4.71GEE645 pKa = 4.16LVIPEE650 pKa = 4.57RR651 pKa = 11.84GDD653 pKa = 3.33PYY655 pKa = 11.05HH656 pKa = 6.89RR657 pKa = 11.84AFMDD661 pKa = 3.39AMSVATRR668 pKa = 11.84RR669 pKa = 11.84AYY671 pKa = 10.34DD672 pKa = 3.61YY673 pKa = 11.41LSGGEE678 pKa = 4.02VLPVPEE684 pKa = 5.37ADD686 pKa = 3.47LASVARR692 pKa = 11.84SITT695 pKa = 3.32

Molecular weight: 75.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4SUG9|A0A2R4SUG9_9VIRU Uncharacterized protein OS=Penicillium digitatum polymycoviruses 1 OX=2164101 PE=4 SV=1
MM1 pKa = 7.7SSPTAPRR8 pKa = 11.84LVSVEE13 pKa = 3.99KK14 pKa = 10.88AKK16 pKa = 11.05LIADD20 pKa = 4.64LDD22 pKa = 3.85QSVVDD27 pKa = 4.29VILRR31 pKa = 11.84LSSLGVKK38 pKa = 9.77PDD40 pKa = 4.83GIIDD44 pKa = 3.58YY45 pKa = 9.78CNRR48 pKa = 11.84VALDD52 pKa = 3.8EE53 pKa = 4.38PAPAVVAGGAKK64 pKa = 9.96PLVIQAYY71 pKa = 10.23SFLSDD76 pKa = 4.39KK77 pKa = 10.79SICVDD82 pKa = 3.09KK83 pKa = 11.22FGMEE87 pKa = 4.3LSAAAAALEE96 pKa = 4.1TLKK99 pKa = 10.48RR100 pKa = 11.84DD101 pKa = 3.56PEE103 pKa = 3.88EE104 pKa = 3.95GRR106 pKa = 11.84RR107 pKa = 11.84IVHH110 pKa = 6.47KK111 pKa = 10.74AVADD115 pKa = 3.86HH116 pKa = 4.94QAKK119 pKa = 10.21RR120 pKa = 11.84GSPKK124 pKa = 10.29PIVVNLAGLPSSRR137 pKa = 11.84GGPKK141 pKa = 9.69TGGDD145 pKa = 3.27GGKK148 pKa = 10.26AEE150 pKa = 4.62LSEE153 pKa = 4.38LMRR156 pKa = 11.84RR157 pKa = 11.84NQALAGAYY165 pKa = 9.69AFVAEE170 pKa = 4.43EE171 pKa = 4.39HH172 pKa = 6.66GPPGPEE178 pKa = 3.46RR179 pKa = 11.84FRR181 pKa = 11.84VLLGGNLAVVGQNKK195 pKa = 9.51ACAVKK200 pKa = 10.18AAALVRR206 pKa = 11.84ILGRR210 pKa = 11.84RR211 pKa = 11.84HH212 pKa = 3.8QTLVKK217 pKa = 9.72YY218 pKa = 10.23VRR220 pKa = 11.84GYY222 pKa = 10.53IPGKK226 pKa = 10.5ALLPSDD232 pKa = 5.25KK233 pKa = 10.2IPPLIYY239 pKa = 10.46DD240 pKa = 4.51GIIAPDD246 pKa = 3.77EE247 pKa = 4.5KK248 pKa = 10.88PPSDD252 pKa = 3.6EE253 pKa = 4.89SKK255 pKa = 10.72GQSTVGG261 pKa = 3.15

Molecular weight: 27.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2328

261

760

582.0

63.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.868 ± 1.516

1.375 ± 0.247

6.271 ± 0.085

5.498 ± 0.361

3.393 ± 0.457

8.333 ± 0.849

2.706 ± 0.325

3.179 ± 0.494

3.351 ± 0.823

9.622 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.329

2.448 ± 0.277

5.412 ± 0.869

2.448 ± 0.241

8.247 ± 0.345

6.787 ± 0.286

4.94 ± 0.685

9.149 ± 0.328

0.816 ± 0.135

3.05 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski