Arthrobacter phage Kumotta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6ENC3|A0A4Y6ENC3_9CAUD Major capsid protein OS=Arthrobacter phage Kumotta OX=2588498 GN=7 PE=4 SV=1
MM1 pKa = 6.52TTSIPALLEE10 pKa = 4.71PIQEE14 pKa = 4.14RR15 pKa = 11.84LGAAEE20 pKa = 3.84GRR22 pKa = 11.84VRR24 pKa = 11.84NPMFGDD30 pKa = 3.41VLRR33 pKa = 11.84IVWTTYY39 pKa = 10.96YY40 pKa = 10.61FGADD44 pKa = 3.93LEE46 pKa = 4.51WHH48 pKa = 6.06NARR51 pKa = 11.84SGEE54 pKa = 4.08VEE56 pKa = 3.97NASTLVAEE64 pKa = 4.88MEE66 pKa = 4.13ASEE69 pKa = 5.87GDD71 pKa = 4.1WEE73 pKa = 4.17WLIPGSGADD82 pKa = 3.61TARR85 pKa = 11.84LLAAVKK91 pKa = 10.37AVVALHH97 pKa = 6.16EE98 pKa = 4.73CDD100 pKa = 3.66VDD102 pKa = 4.58FGKK105 pKa = 8.92CTDD108 pKa = 5.34CDD110 pKa = 4.27DD111 pKa = 3.97EE112 pKa = 4.8WPCLTVSAVEE122 pKa = 3.6AALRR126 pKa = 11.84DD127 pKa = 3.97DD128 pKa = 3.83AA129 pKa = 6.91

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6ELE7|A0A4Y6ELE7_9CAUD Uncharacterized protein OS=Arthrobacter phage Kumotta OX=2588498 GN=36 PE=4 SV=1
MM1 pKa = 7.61SYY3 pKa = 10.81ARR5 pKa = 11.84HH6 pKa = 5.99EE7 pKa = 4.22RR8 pKa = 11.84TIPAMNSPDD17 pKa = 3.52EE18 pKa = 4.35PAPLSAIAAQRR29 pKa = 11.84FRR31 pKa = 11.84IGLAALGLNDD41 pKa = 3.47SEE43 pKa = 4.45AARR46 pKa = 11.84RR47 pKa = 11.84LGWPQPNVQRR57 pKa = 11.84KK58 pKa = 5.16THH60 pKa = 6.18HH61 pKa = 5.89KK62 pKa = 8.48TVMSLAHH69 pKa = 6.72LEE71 pKa = 4.46HH72 pKa = 6.64IQRR75 pKa = 11.84TLGIPVRR82 pKa = 11.84YY83 pKa = 9.56LLGFDD88 pKa = 4.67DD89 pKa = 5.86DD90 pKa = 4.63FPGMLKK96 pKa = 10.21RR97 pKa = 11.84RR98 pKa = 3.89

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

12628

45

1142

168.4

18.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.435 ± 0.381

1.053 ± 0.207

6.113 ± 0.265

6.367 ± 0.349

3.08 ± 0.296

8.75 ± 0.463

2.154 ± 0.165

4.601 ± 0.223

4.553 ± 0.224

7.634 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.134

3.128 ± 0.198

5.504 ± 0.221

3.651 ± 0.172

6.367 ± 0.501

5.401 ± 0.261

6.731 ± 0.249

6.668 ± 0.286

2.138 ± 0.138

2.423 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski