Sicyonia brevirostris associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL94|A0A0K1RL94_9CIRC Putative replication initiation protein (Fragment) OS=Sicyonia brevirostris associated circular virus OX=1692263 PE=4 SV=1
II1 pKa = 6.56GQNGLKK7 pKa = 10.12DD8 pKa = 3.82NPIIPYY14 pKa = 8.94TMSYY18 pKa = 10.43PMPNPQQRR26 pKa = 11.84EE27 pKa = 4.1VLASTFRR34 pKa = 11.84TLVKK38 pKa = 10.61ANPDD42 pKa = 2.69QWTNSPSFEE51 pKa = 4.39KK52 pKa = 10.19LCRR55 pKa = 11.84MYY57 pKa = 10.61RR58 pKa = 11.84CYY60 pKa = 11.23DD61 pKa = 4.19LIKK64 pKa = 10.53DD65 pKa = 3.89RR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 4.78QFITVALPQTYY79 pKa = 10.01DD80 pKa = 3.19LKK82 pKa = 11.31KK83 pKa = 10.47LVSQIEE89 pKa = 3.92NEE91 pKa = 4.23LRR93 pKa = 11.84YY94 pKa = 10.51DD95 pKa = 4.19WIIGAYY101 pKa = 9.49VRR103 pKa = 11.84VEE105 pKa = 4.06NFSDD109 pKa = 3.22TGEE112 pKa = 4.06NLHH115 pKa = 6.71LHH117 pKa = 5.91ILKK120 pKa = 10.52DD121 pKa = 3.61GQYY124 pKa = 9.17TKK126 pKa = 10.65TKK128 pKa = 10.02IIRR131 pKa = 11.84DD132 pKa = 3.86LSRR135 pKa = 11.84KK136 pKa = 9.43FKK138 pKa = 10.85VEE140 pKa = 3.78KK141 pKa = 10.89NFINVKK147 pKa = 9.99SSNKK151 pKa = 9.51EE152 pKa = 3.04VDD154 pKa = 3.07YY155 pKa = 11.01EE156 pKa = 3.97NRR158 pKa = 11.84YY159 pKa = 10.6NYY161 pKa = 10.08ILGNKK166 pKa = 8.58KK167 pKa = 10.26DD168 pKa = 3.53AGKK171 pKa = 10.73LEE173 pKa = 4.0NVQKK177 pKa = 10.76DD178 pKa = 3.76KK179 pKa = 11.03EE180 pKa = 4.19WRR182 pKa = 11.84EE183 pKa = 3.8NNDD186 pKa = 2.62INEE189 pKa = 4.22IYY191 pKa = 10.85NLL193 pKa = 3.77

Molecular weight: 22.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL94|A0A0K1RL94_9CIRC Putative replication initiation protein (Fragment) OS=Sicyonia brevirostris associated circular virus OX=1692263 PE=4 SV=1
MM1 pKa = 7.79PRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.92KK6 pKa = 9.41LTGRR10 pKa = 11.84VRR12 pKa = 11.84RR13 pKa = 11.84TTHH16 pKa = 6.3LSSNAKK22 pKa = 10.0KK23 pKa = 9.73EE24 pKa = 3.77VRR26 pKa = 11.84AIARR30 pKa = 11.84KK31 pKa = 8.1VARR34 pKa = 11.84SMPEE38 pKa = 3.45VKK40 pKa = 9.45NTVFTEE46 pKa = 4.07EE47 pKa = 4.56NIQLYY52 pKa = 9.21HH53 pKa = 6.73NKK55 pKa = 9.43VLYY58 pKa = 9.79LGKK61 pKa = 9.47WLACQQGTADD71 pKa = 4.48PNDD74 pKa = 3.47ASSRR78 pKa = 11.84LMRR81 pKa = 11.84VGDD84 pKa = 3.9EE85 pKa = 3.94LLLRR89 pKa = 11.84NINVRR94 pKa = 11.84FWLSNKK100 pKa = 8.87SDD102 pKa = 4.11RR103 pKa = 11.84PNVMYY108 pKa = 10.46KK109 pKa = 10.46AYY111 pKa = 10.51LFWYY115 pKa = 7.39DD116 pKa = 3.29TGATLSDD123 pKa = 3.27TFNYY127 pKa = 7.39FTNTNKK133 pKa = 10.21MIDD136 pKa = 3.22RR137 pKa = 11.84VNNEE141 pKa = 4.33NISVIDD147 pKa = 3.37QKK149 pKa = 10.05TIFSQNNYY157 pKa = 10.16AVDD160 pKa = 3.81ANNHH164 pKa = 5.25EE165 pKa = 4.25RR166 pKa = 11.84SQLCTLKK173 pKa = 11.0ASWKK177 pKa = 8.42GKK179 pKa = 10.11KK180 pKa = 9.41IIYY183 pKa = 9.8DD184 pKa = 3.61EE185 pKa = 4.98GGQAPKK191 pKa = 10.61KK192 pKa = 9.75RR193 pKa = 11.84DD194 pKa = 3.65LGVMIVCYY202 pKa = 9.99DD203 pKa = 3.41AYY205 pKa = 10.39GTLQTDD211 pKa = 4.6NIASLAYY218 pKa = 9.62NGTVAYY224 pKa = 10.04QDD226 pKa = 3.52PP227 pKa = 3.98

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

420

193

227

210.0

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.238 ± 1.798

1.19 ± 0.105

6.429 ± 0.209

5.0 ± 1.179

2.857 ± 0.171

4.286 ± 0.447

1.429 ± 0.085

6.19 ± 1.074

8.81 ± 0.703

8.095 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.4

9.524 ± 0.218

3.333 ± 0.551

4.286 ± 0.256

6.667 ± 0.305

5.238 ± 0.39

5.952 ± 0.876

5.952 ± 0.524

1.667 ± 0.076

5.714 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski