Microbacterium sp. cf046

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6MGU5|A0A1I6MGU5_9MICO Uncharacterized conserved protein circularly permuted ATPgrasp superfamily OS=Microbacterium sp. cf046 OX=1761803 GN=SAMN04487846_3000 PE=4 SV=1
MM1 pKa = 7.34IRR3 pKa = 11.84WKK5 pKa = 10.39KK6 pKa = 9.6AAAAAAIAVTTALAVTACAGGSTDD30 pKa = 4.64GGGEE34 pKa = 4.15GAGTLTLGAIGAPTTFDD51 pKa = 3.58PAGSQWGNLSPFYY64 pKa = 10.45QAVFDD69 pKa = 4.24TLLLATPEE77 pKa = 4.18GTIEE81 pKa = 3.82PWLATEE87 pKa = 4.74WSYY90 pKa = 12.27NDD92 pKa = 4.13DD93 pKa = 3.76NTVLTLTLRR102 pKa = 11.84DD103 pKa = 4.01DD104 pKa = 3.81VTFSDD109 pKa = 4.64GSKK112 pKa = 9.12LTADD116 pKa = 3.55VVAKK120 pKa = 10.34NLQRR124 pKa = 11.84FKK126 pKa = 11.28DD127 pKa = 3.84GTSPDD132 pKa = 3.15AGYY135 pKa = 9.51FAGVASFEE143 pKa = 4.52APDD146 pKa = 3.76DD147 pKa = 3.88TTVVITLSAPDD158 pKa = 4.03PAMLNYY164 pKa = 8.63LTRR167 pKa = 11.84DD168 pKa = 3.42PGLVGAEE175 pKa = 4.17ASLDD179 pKa = 3.8SADD182 pKa = 3.89AATNPVGSGPYY193 pKa = 9.88ILDD196 pKa = 3.24TAATVTGTTYY206 pKa = 10.95SYY208 pKa = 10.95TKK210 pKa = 10.86NPDD213 pKa = 2.68YY214 pKa = 10.68WNPDD218 pKa = 2.66VQYY221 pKa = 11.21YY222 pKa = 10.74DD223 pKa = 3.48NLVINVLSDD232 pKa = 3.12PTAALNAIKK241 pKa = 10.33AGEE244 pKa = 4.02ANGVKK249 pKa = 10.06LANNDD254 pKa = 3.17ALTEE258 pKa = 4.04VEE260 pKa = 4.05AAGWTVNANEE270 pKa = 5.21LDD272 pKa = 3.93FQGLLLLDD280 pKa = 4.02RR281 pKa = 11.84AGTMDD286 pKa = 4.61PALADD291 pKa = 3.49VKK293 pKa = 10.74VRR295 pKa = 11.84QAINYY300 pKa = 9.26AFDD303 pKa = 4.42RR304 pKa = 11.84DD305 pKa = 3.9GLLTALQTDD314 pKa = 3.95HH315 pKa = 6.51GTVTTQVFPATSDD328 pKa = 3.41AYY330 pKa = 10.78DD331 pKa = 4.22PEE333 pKa = 6.02LDD335 pKa = 3.64SYY337 pKa = 11.84YY338 pKa = 10.91DD339 pKa = 3.87YY340 pKa = 11.48DD341 pKa = 3.78PEE343 pKa = 4.34KK344 pKa = 10.88AKK346 pKa = 10.73EE347 pKa = 3.92LLAEE351 pKa = 4.43AGYY354 pKa = 9.92PDD356 pKa = 4.57GLSISMPTVSVLSATTYY373 pKa = 10.66TLVAQQLADD382 pKa = 3.38VGITVEE388 pKa = 4.17ATDD391 pKa = 3.72VPVGNFIADD400 pKa = 3.62MLAPKK405 pKa = 10.5YY406 pKa = 9.27PATFMALEE414 pKa = 4.26QNPDD418 pKa = 2.7WQLIQFMIAPNAVFNPFKK436 pKa = 10.96YY437 pKa = 9.93QDD439 pKa = 3.87PQVDD443 pKa = 4.07EE444 pKa = 4.64YY445 pKa = 10.91IKK447 pKa = 10.06EE448 pKa = 3.81IQYY451 pKa = 11.28GDD453 pKa = 4.06EE454 pKa = 3.93ATQASVAKK462 pKa = 9.68EE463 pKa = 3.68LNTYY467 pKa = 9.79IVEE470 pKa = 4.46QAWFAPFYY478 pKa = 10.24RR479 pKa = 11.84VQGSVATDD487 pKa = 3.11ANTTVEE493 pKa = 4.19MLPTNAYY500 pKa = 8.08PAIYY504 pKa = 10.15DD505 pKa = 3.79FQPKK509 pKa = 7.63QQ510 pKa = 3.15

Molecular weight: 54.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6KT77|A0A1I6KT77_9MICO DNA-binding response regulator NarL/FixJ family contains REC and HTH domains OS=Microbacterium sp. cf046 OX=1761803 GN=SAMN04487846_0907 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.44RR3 pKa = 11.84FWLFLIPGVILSAVGLVWTLQGLNVLGGSAMSGSPLWATIGPIVLVVGVVLIVIAIVIAVRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84TQPP70 pKa = 2.79

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3641

0

3641

1231243

37

2020

338.2

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.525 ± 0.059

0.486 ± 0.009

6.424 ± 0.037

5.643 ± 0.039

3.229 ± 0.026

8.925 ± 0.035

1.98 ± 0.02

4.82 ± 0.031

1.788 ± 0.027

10.004 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.016

1.872 ± 0.02

5.545 ± 0.03

2.696 ± 0.019

7.286 ± 0.043

5.614 ± 0.026

5.956 ± 0.032

8.762 ± 0.038

1.612 ± 0.019

2.034 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski