Zoogloea ramigera

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y4CUU6|A0A4Y4CUU6_ZOORA CheW-like domain-containing protein OS=Zoogloea ramigera OX=350 GN=ZRA01_27540 PE=4 SV=1
MM1 pKa = 7.19VIWMDD6 pKa = 3.46MQSGEE11 pKa = 4.08RR12 pKa = 11.84FYY14 pKa = 11.21DD15 pKa = 3.76EE16 pKa = 4.18PQEE19 pKa = 4.34AVGSVYY25 pKa = 10.92DD26 pKa = 5.34DD27 pKa = 3.56EE28 pKa = 4.81VLNANLVPPEE38 pKa = 4.16LALGLQEE45 pKa = 4.8IPLPEE50 pKa = 4.64HH51 pKa = 6.6AVHH54 pKa = 6.89PVPTDD59 pKa = 3.26VEE61 pKa = 4.38AFLSAVYY68 pKa = 10.31RR69 pKa = 11.84FQEE72 pKa = 3.91

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y4CYZ5|A0A4Y4CYZ5_ZOORA Flagella basal body P-ring formation protein FlgA OS=Zoogloea ramigera OX=350 GN=flgA PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.37SKK4 pKa = 10.45QGRR7 pKa = 11.84FALALASLLLAGSAWAQASPEE28 pKa = 4.06AAPGAGRR35 pKa = 11.84GMGPGAGMGPGHH47 pKa = 7.11CMQADD52 pKa = 4.02PAQPNCGWRR61 pKa = 11.84MSRR64 pKa = 11.84RR65 pKa = 11.84NSPGYY70 pKa = 11.01GLMTPEE76 pKa = 4.37EE77 pKa = 4.2RR78 pKa = 11.84AAHH81 pKa = 4.78QNRR84 pKa = 11.84LRR86 pKa = 11.84SFQSRR91 pKa = 11.84EE92 pKa = 3.35EE93 pKa = 4.08CLAYY97 pKa = 9.81MQDD100 pKa = 2.88HH101 pKa = 6.79HH102 pKa = 8.53AQMGEE107 pKa = 3.84RR108 pKa = 11.84AKK110 pKa = 11.01AQGKK114 pKa = 5.98TLPAPRR120 pKa = 11.84MRR122 pKa = 11.84LCDD125 pKa = 3.9RR126 pKa = 11.84LPPASGRR133 pKa = 3.74

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3918

0

3918

1310892

40

6982

334.6

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.888 ± 0.055

0.959 ± 0.015

5.523 ± 0.029

5.778 ± 0.035

3.57 ± 0.021

8.405 ± 0.04

2.223 ± 0.021

4.487 ± 0.027

3.238 ± 0.035

11.089 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.021

2.52 ± 0.023

5.384 ± 0.03

3.542 ± 0.023

7.334 ± 0.038

5.212 ± 0.041

4.799 ± 0.047

7.346 ± 0.028

1.36 ± 0.017

2.146 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski