Staphylococcus phage phiMR25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2ZYW6|B2ZYW6_BPMR2 Uncharacterized protein orf28 OS=Staphylococcus phage phiMR25 OX=487152 GN=orf28 PE=4 SV=1
MM1 pKa = 7.45ATQKK5 pKa = 10.54QVDD8 pKa = 4.13YY9 pKa = 11.64VMSLQEE15 pKa = 3.87QLEE18 pKa = 4.35LEE20 pKa = 4.45DD21 pKa = 3.95CEE23 pKa = 5.56KK24 pKa = 10.67YY25 pKa = 9.42TDD27 pKa = 3.83EE28 pKa = 4.01QVKK31 pKa = 10.4AMSHH35 pKa = 6.03KK36 pKa = 10.06EE37 pKa = 3.72VSNVIEE43 pKa = 4.41NYY45 pKa = 9.42KK46 pKa = 10.77ASISNEE52 pKa = 3.8EE53 pKa = 4.63LYY55 pKa = 10.85DD56 pKa = 3.48EE57 pKa = 4.93CMSFGLPNCC66 pKa = 4.51

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2ZZ08|B2ZZ08_BPMR2 Uncharacterized protein orf70 OS=Staphylococcus phage phiMR25 OX=487152 GN=orf70 PE=4 SV=1
MM1 pKa = 7.86IVMVITIISVFVISAILLFYY21 pKa = 10.74LSAVTFTAVRR31 pKa = 11.84SYY33 pKa = 11.3DD34 pKa = 3.69YY35 pKa = 10.74NVKK38 pKa = 10.44FSVPTLFAIPLILVSTHH55 pKa = 4.51IKK57 pKa = 9.98IYY59 pKa = 9.38NTYY62 pKa = 10.35KK63 pKa = 10.77LSDD66 pKa = 3.39RR67 pKa = 11.84KK68 pKa = 10.44KK69 pKa = 9.55AFRR72 pKa = 11.84MLLFIFKK79 pKa = 9.78KK80 pKa = 9.98YY81 pKa = 9.61PVAIAMFLTIIATNIAEE98 pKa = 4.38QNVKK102 pKa = 10.33LSNGATYY109 pKa = 10.81KK110 pKa = 9.71KK111 pKa = 9.22TEE113 pKa = 3.82NLQQKK118 pKa = 9.06KK119 pKa = 8.76RR120 pKa = 11.84KK121 pKa = 9.83KK122 pKa = 9.5MINKK126 pKa = 8.34VLQSNTYY133 pKa = 10.76GEE135 pKa = 4.31MLLGII140 pKa = 4.83

Molecular weight: 16.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13602

48

1154

194.3

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.69 ± 0.409

0.559 ± 0.087

6.22 ± 0.36

7.639 ± 0.486

4.426 ± 0.232

5.521 ± 0.254

1.559 ± 0.146

7.271 ± 0.338

9.197 ± 0.321

7.543 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.138

6.698 ± 0.257

2.86 ± 0.221

3.808 ± 0.174

4.154 ± 0.209

5.757 ± 0.225

6.462 ± 0.254

6.271 ± 0.232

1.228 ± 0.165

4.61 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski