Hyphomicrobium facile

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7NBE3|A0A1I7NBE3_9RHIZ Ca-activated chloride channel family protein OS=Hyphomicrobium facile OX=51670 GN=SAMN04488557_1458 PE=4 SV=1
MM1 pKa = 7.43SNAIVLEE8 pKa = 4.01NEE10 pKa = 4.38KK11 pKa = 10.58PGAPQSEE18 pKa = 4.36WMLQPGEE25 pKa = 4.14ASSSIEE31 pKa = 3.85GFTTSMSTNLGSTVNFKK48 pKa = 10.87INTDD52 pKa = 3.63SNDD55 pKa = 3.23YY56 pKa = 10.85RR57 pKa = 11.84IDD59 pKa = 3.43IYY61 pKa = 11.26RR62 pKa = 11.84LGYY65 pKa = 10.15YY66 pKa = 10.35GGDD69 pKa = 3.35GATLVTSMEE78 pKa = 4.0HH79 pKa = 5.53QGAVNQPNPITDD91 pKa = 3.69PTTGNIDD98 pKa = 3.35AGNWSVTDD106 pKa = 3.17SWNIPTNATSGVYY119 pKa = 9.05IAKK122 pKa = 8.91LTRR125 pKa = 11.84LDD127 pKa = 3.6GTSGEE132 pKa = 4.23NIIPFVVTNNASTSDD147 pKa = 3.87IIFQTSDD154 pKa = 3.65STWQAYY160 pKa = 7.28NAWGGGSFYY169 pKa = 10.7TDD171 pKa = 2.96TAQNISYY178 pKa = 9.52NRR180 pKa = 11.84PIQTPAGPDD189 pKa = 3.23DD190 pKa = 4.05TTASGPWDD198 pKa = 3.48FVFGEE203 pKa = 4.62EE204 pKa = 4.69LPAIYY209 pKa = 9.99FLEE212 pKa = 4.33KK213 pKa = 10.29NGYY216 pKa = 8.72DD217 pKa = 3.22VSYY220 pKa = 11.14QSGIDD225 pKa = 3.3TATNGSLLLNHH236 pKa = 7.02KK237 pKa = 10.38AFLSVGHH244 pKa = 6.7DD245 pKa = 4.03EE246 pKa = 4.43YY247 pKa = 10.1WTAQQQNNVRR257 pKa = 11.84AARR260 pKa = 11.84DD261 pKa = 3.38AGVSLNFWSGNEE273 pKa = 4.32SYY275 pKa = 7.43WTTEE279 pKa = 4.15LLPSIDD285 pKa = 4.06GSGDD289 pKa = 3.42PNRR292 pKa = 11.84TLVTYY297 pKa = 7.8KK298 pKa = 10.33TSATGLQNPDD308 pKa = 3.83GVWTGATRR316 pKa = 11.84DD317 pKa = 3.77PAGGLIPEE325 pKa = 4.88NGLSGQMYY333 pKa = 9.91YY334 pKa = 10.94VDD336 pKa = 4.08WDD338 pKa = 3.92HH339 pKa = 6.41NTPLNPITVPYY350 pKa = 10.23KK351 pKa = 10.05YY352 pKa = 10.5AQLNIWKK359 pKa = 8.31NTEE362 pKa = 3.88VANLQPGQTYY372 pKa = 8.74TSQSDD377 pKa = 3.82YY378 pKa = 11.36LGYY381 pKa = 10.49EE382 pKa = 3.65WDD384 pKa = 3.85VNATSSEE391 pKa = 4.1VANNGFSPAGLVPLSSTTVTSDD413 pKa = 3.03AVSNAYY419 pKa = 9.58ILTGLITGTATHH431 pKa = 6.72NLTLYY436 pKa = 9.55RR437 pKa = 11.84APSGALVFAAGSIMWSWSLSSLHH460 pKa = 6.21TPGPDD465 pKa = 3.74GSPTDD470 pKa = 4.26APADD474 pKa = 3.59PAFQQAMVNLLAQEE488 pKa = 5.53GIQPQSLDD496 pKa = 3.31SSLVLASQSTDD507 pKa = 3.54FLPPTTVISTNTAAQFVAGQLVTITGTATDD537 pKa = 3.24QGGGVVAGVEE547 pKa = 4.22VSTDD551 pKa = 3.34GGNTWHH557 pKa = 6.84EE558 pKa = 4.4ANFAAAAANVSWSYY572 pKa = 9.5TWTPAATGSYY582 pKa = 9.18TILARR587 pKa = 11.84SVDD590 pKa = 3.86DD591 pKa = 4.07VANLAHH597 pKa = 7.26PLLSPNFSITDD608 pKa = 4.39FYY610 pKa = 11.7SNFAVSQGWASADD623 pKa = 3.63TLRR626 pKa = 11.84TLADD630 pKa = 3.78FNGDD634 pKa = 2.86GRR636 pKa = 11.84ADD638 pKa = 3.54FVGFGASTVYY648 pKa = 10.86ASASLGDD655 pKa = 3.46ASQYY659 pKa = 11.25NGGASFSTSLRR670 pKa = 11.84SLANDD675 pKa = 3.39FTIAQGYY682 pKa = 5.53TAANQRR688 pKa = 11.84GMDD691 pKa = 3.7FVGNLSSSPTDD702 pKa = 3.22HH703 pKa = 7.65FDD705 pKa = 3.55TLWAVGADD713 pKa = 3.56GFHH716 pKa = 6.25YY717 pKa = 10.71ASVASSGAATGSKK730 pKa = 10.16VYY732 pKa = 11.14NNFGLAQGWTQTYY745 pKa = 9.66GVDD748 pKa = 3.29VAFLSKK754 pKa = 10.49SDD756 pKa = 3.68AYY758 pKa = 10.87ASIVGFGVSGLWVGQQAFNPTATPAASYY786 pKa = 10.07IAAGSQTLGGASGWDD801 pKa = 3.34STLDD805 pKa = 3.08IQTFRR810 pKa = 11.84DD811 pKa = 3.59YY812 pKa = 10.7RR813 pKa = 11.84GNMIDD818 pKa = 4.73LNNDD822 pKa = 4.11GIADD826 pKa = 4.17FVGMGPTGLEE836 pKa = 3.72YY837 pKa = 10.33AYY839 pKa = 10.66GQFTAGATAGSQVYY853 pKa = 10.58SLGAIQKK860 pKa = 10.14GSTGTNIDD868 pKa = 3.54FGRR871 pKa = 11.84DD872 pKa = 3.57QGWDD876 pKa = 2.95TSTSDD881 pKa = 5.43RR882 pKa = 11.84ILADD886 pKa = 3.04INGDD890 pKa = 3.11GRR892 pKa = 11.84LDD894 pKa = 3.41VVAFGDD900 pKa = 3.44AGVWVSIGQQANADD914 pKa = 3.42GSGAFGAAYY923 pKa = 10.1LAMANFGTQQGWSTAVNTRR942 pKa = 11.84VLGDD946 pKa = 3.56LYY948 pKa = 11.45GNGKK952 pKa = 9.13IDD954 pKa = 3.28IVGFGADD961 pKa = 3.48YY962 pKa = 10.54TFIATPTVDD971 pKa = 2.96AATGHH976 pKa = 4.58VTFGISEE983 pKa = 4.26SLHH986 pKa = 6.91AYY988 pKa = 6.86GTNEE992 pKa = 4.52GYY994 pKa = 10.36SPSQNFRR1001 pKa = 11.84GVADD1005 pKa = 3.71VNGTGVDD1012 pKa = 4.05SIVVSSLSNTQIVSHH1027 pKa = 5.68VV1028 pKa = 3.29

Molecular weight: 107.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7NUV3|A0A1I7NUV3_9RHIZ Uncharacterized protein OS=Hyphomicrobium facile OX=51670 GN=SAMN04488557_3666 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.26RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.73GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MLTAGGQKK29 pKa = 9.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.51GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4102

0

4102

1277010

29

2151

311.3

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.01 ± 0.052

0.864 ± 0.014

5.575 ± 0.029

5.612 ± 0.037

3.813 ± 0.026

8.156 ± 0.037

1.985 ± 0.018

5.535 ± 0.022

4.096 ± 0.036

9.746 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.016

2.891 ± 0.022

5.236 ± 0.03

3.049 ± 0.022

6.733 ± 0.042

6.106 ± 0.028

5.363 ± 0.026

7.304 ± 0.031

1.324 ± 0.016

2.313 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski